Comparison of anatomic characteristics between in vitro cultured plantlets and naturally grown parental plants in Populus cuspica

Author

Research Institute of F'orests & Ranselands

Abstract

Anatomic  charactristics of elite adult trees of  P. caspica and in vitro derived plants were  compared .
Results showed that plants from natural environment (Genotype 1,2)are  different from Genotype 3 , because of less cuticole and tricomes on epidermis cells in the former(s).
All of the plants showed oxalate calcium crystals in both collenchymatic cells of leaves and parenchymatic cells of petioles. Adult  plants showed vascular bundles with  a scleranshima ring around the phloem , but tissue cultured explants showed  less cuticoles and tricomes on epidermis, small and restricted vessels without scleranshima ring.Also stomata were anemositic and  bulgy  in all of the test samples.method of search is the microtomic sectioned from leaves and petioles , stained them , fixationed and microscopic studied  between adult trees and tissue cultured species.

Keywords