Comparison of anatomic characteristics between in vitro cultured plantlets and naturally grown parental plants in Populus cuspica

Emam, M.

doi:10.22092/ijrfpbgr.2001.115873

Comparison of anatomic characteristics between in vitro cultured plantlets and naturally grown parental plants in Populus cuspica

Author

M. Emam

Research Institute of F'orests & Ranselands

10.22092/ijrfpbgr.2001.115873

Abstract

Anatomic charactristics of elite adult trees of P. caspica and in vitro derived plants were compared .
Results showed that plants from natural environment (Genotype 1,2)are different from Genotype 3 , because of less cuticole and tricomes on epidermis cells in the former(s).
All of the plants showed oxalate calcium crystals in both collenchymatic cells of leaves and parenchymatic cells of petioles. Adult plants showed vascular bundles with a scleranshima ring around the phloem , but tissue cultured explants showed less cuticoles and tricomes on epidermis, small and restricted vessels without scleranshima ring.Also stomata were anemositic and bulgy in all of the test samples.method of search is the microtomic sectioned from leaves and petioles , stained them , fixationed and microscopic studied between adult trees and tissue cultured species.

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