Evaluation of genetic variation in new somaclons of Populus euphratica OLIV

Authors

1 Research Institute of Forests and Rangelands, P.o. Box: 13185-116, Tehran, Iran

2 Populus euphratica Oliv, gametoclonal, tetrad- uninucleate, aneuploid, polyPloid and haPloid

Abstract

In order to evaluate induced genetic variation of Populus euphratica Oliv. through cell culture, cytological and isozyme electrophrosis analysis were carried out. To induce genetic variation, immature inflorences of female tree containing microspore mother cells were isolated and cultured. Significant different was observed between growth regulator hormone treatment for callus induction at 0.01 level. MS medium supplemented with 2 mg/l 2,4-D and 0.1 mg/l kinetin produced higher callus from immature inflorences and showed significant differences with the other hormonal treatments at 0.01 level..The calli were subculture for8 month in MS medium to increase variation among regenerated plantlets. Higher plant regeneration (75.5 %) was observed in solidified MS medium containing 3 mg/l BA and 0.5mg/l NAA. High degree of morphological behavior (leaves and stems) were observed among regenerated plants. Cytological analysis on root tip of regenerated plantlets showed the presence of haploid, diploid, aneuploid and polyploid plantlets.

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