Study of variation and seed deterioration of Bromus tbmentellrrs germplasm, in natural resources genebank

Authors

1 Ph.D. Postgraduate student of Science & Research Campus of Islamic Azad University

2 Scientific Board Member of Research lnistitute of Forests and Rangelands

Abstract

In order to examine and evaluate the variation and process of deterioration of accessions of Bromus tomentellus seeds, which have been collected in natural resources genebank, research programs were carried out using 22 accessions. All of the accessions were examined in a complete randomized design with three replications under two different conditions, germinator and glasshouse.
The recorded traits of the Germinator test included, germination percentage (G.P.), speed of germination and vigor. The recorded traits of the test in glasshous, included G.P., speed of germination, plant height, number of tillers and root length in 40 days after planting.
Significant differences were found between accessions for all of the studied traits. Variation between accessions was significant in one or both of the environments.
The results of correlation coefficient study showed that, 1000 seed weight could affect G.P. and preliminary growth of plants in the both environments. Speed of germination, could be very good indicator of seed vigor. This result was confirmed by regression model. Negative correlation was found between reduction of G.P., speed of germination and seed vigor in germinator condition and G.P., speed of germination and plant height in glasshouse condition. Therefore, seed deterioration not only reduces G.P. but also decreases the speed of germination and seed vigor.
In all of the Bromus tomentellus accessions, because stored samples of each year were different from other years, therefore reduction of G.P., was highly affected by origin of the accessions. It was not possible to differentiate between the effect of maintaining condition and origin of the seeds. It was suggested that, for better genebank management, accessions, have to be considered as regeneration unites of collected seeds.

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