Karyotypic investigations in several populations of Lorium multiflorum and L. rigidum

Authors

1 Research Institute of Forests ancl Rangelands, Tehran, Iran

2 Payam Noor University graduate student. Tchran, Iran

Abstract

Application of Lalium germplasm in breeding of available Lalium species requires sufficient information about different aspects, including variIous karyotypic characteristics. For this reason, nine populations of Lalium multiflarum and L. rigidum species, randomly taken from Natural Resources Gene Bank, were investigated for karyotypic characteristics. Five metaphase cells were studied from each population for several karyotypic characteristics, including long and short arm length, by which total length of the chromosomes, long arm to short arm and short arm to long arm ratios were calculated. The number and locations of the possible satellites were also identified. Using several available estimation formula, karyotypic asymmetry of the populations were also investigated. Using Levan ratios, karyotypic formula of the populations and species were also determined.
Amongst the studied populations, two populations were tetraploid, five populations were diploid and two populations were a mixture of tetraploid and diploid genotypes. Several populations had one to two satellites on their chromosomes. Most of the observed chromosomes of the populations were metacentric, in which the centromers were located in median region. Based on one of the karyotypic asymmetry measurment parameters, DRL, within the tetraploid populations, two populations of L. multiflorum showed the most symmetric and asymmetric karyotypes. Within the diploid populations, a population of L. rigidum species showed the most symmetric and a population of the L. multiflorum species showed the most asymmetric karyotype.

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