Micropropagation of Populus caspica by internode culture

Authors

Research Institute of Forests and Ranselands. P.O.Box 13185-116. Tehran

Abstract

Shoot tips of elite adult trees of Populus caspica in northern forest of Iran (Gillan, Safrabaste) were collected. After sterilization the materials, these explants were cultured  in ½ MS medium with 0.5 mg / lit BA + 0.01 mg / lit IBA.
Enough proliferated shoots were obtained. Internodes with length of 0.5 to 1 cm , were cultured in MS and ½ MS with 3 hormone treatments ( BA : 0.1, 0.5 and 1 mg/lit) with three replications. The differences of proliferation and shoot length growth between treatments were investigated with a factorial experiment and Duncan’s test.  Results showed that  the best medium for internodes’ micropropagation of P. caspica, was MS with 0.5 mg / lit BA. Rooting of shoots were obtained in ½ MS with IBA in 0.5 mg/lit.

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