sexual propagation of Argania spinosa L. Skeels using ovary culture

Authors

1 Research Institute of Forests and Rangelands,Tehran,P.O.Box13185 -l l6'

2 Research Institute of Forestsand Rangelands,Tehran,P.O.Box13185 -l l6'

Abstract

In order to propagate Argania spinosal. skeels, developed ovary culture were used. Seld wittr hard coat were sterilized with 70% of ethanol and rinsed with sterile water. Hard coat was aseptically removed and ovary was isolated. Two type of Isolated ovary (complete and cut ovary explants), were cultured intothe solidified MS, WPM and half-MS (half-concentrated MS rnedium) for embryo germination. Highest embryo germination was observed in cut ovary explant culture (63162%).In spite of no significant differences between media for embryo germination, MS medium produced highest embryo germination for both kind of explants (49152%)' Plantlets were successfully acclimatized and transferred to the greenhouse.

Keywords