Relationship between RuBisCO and photosynthetic efficiency of tissue cultured planflets

Author

Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran.

Abstract

In vitro plants show an abnormality in growth and photosynthetic
performance. In order to understand the role of the main photosynthetic
enzyme (RuBisCO) in photosynthetic efficiency (Pm), and the factors
affecting activity of this enzyme, an experiment was carried out. The
plantlets of wild clrerry were examined for some physiological parameters,
under three growth conditions (in vitro, and ex vitro after 2 ar.td 4 weeks).
The observations showed that under in vitro conditions Pm is too low
compared to ex vitro conditions. changes in Pm of different treatments
were consistent with changes in RuBisCO activity. No significant
difference was observed between leaf RuBisco content in vitro and ex
vitro. No changes in RuBisco activity were observed under different
growth irradiances (50 and 200 pmol photon --' .-t) in in vitro condition,
while under ex vitro conditions higher RuBisCO activity was observed
under higher growth irradiance. Therefore, Pm of in vitro plantlets was
affected by low activity of RuBisCO. As previously reported and the results
of this study also support it, the low activity of this enzyme could be
attributed to presence of sucrose in culture medium and to the low
concentration of magnesium ions in leaf of in vitro plantlets.

Keywords


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