Study of variation and seed deterioration of Onobrychis sativa germplasm, in natural resources genebank

Document Type : Research Paper

Authors

1 Scientific Board Member of Islamic Azad University, Arak unit

2 Scientific Board Member of Research Institute of Forests and Rangelands Karaj

Abstract

In order to examine and evaluate the variation and process of deterioration of accessions of Onobrychis sativa seeds, which have been collected and maintained in natural resources genebank, a research project were carried out using 9 accessions. All of the accessions were examined in a complete randomized design with three replications under using germinator and glasshouse conditions.
The recorded traits of the Germinator test were included, germination percentage (G.P.), speed of germination and vigor. The recorded traits in glasshouse, included G.P., speed of germination, plant height, number of tillers and root length in 40 days after planting.
The results showed significant difference between accessions for all of traits. Variation between accessions was significant in one or both of the environments.
The results of correlation coefficient study shown that Speed of germination, could be very good indicator of seed vigor. This result was confirmed by regression model. Negative correlation was found between reduction of G.P., speed of germination and seed vigor in germinator condition and G.P., speed of germination, plant height, number of tiller and root length in glasshouse condition. Therefore, seed deterioration not only reduces G.P, but also decreases the speed of germination and seed vigor.
In all of the Onobrychis sativa accessions, because stored samples of each year were different from other years therefore, reduction of G.P., was highly affected by origin of accessions. It was not possible to differentiate between the effect of maintaining condition and origin of seed. It was suggested that, for better genebank management, accessions, have to be considered as regeneration unites of collected seeds.

Keywords

References

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