In vitro proliferation of date palm (Phoneix dactylifera L. cv. Mazafeti) plantlet derived from embryo culture

Document Type : Research Paper

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Abstract

In order to determine in vitro regeneration potential of date palm (Phoneix dactylifera L. cv. Mazafati), isolated embryos from immature fruits were transferred to hormone free half-concentration MS medium and MS containing growth regulator hormone. To stimulate meristemic cells for adventitious bud initiation, isolated explants in 1 to 3 cm height were vertically cultured on MS medium containing 0.4 mg/l thiamine Hcl, 100 mg/l inositol 30 g/l sucrose and different combination of growth regulators. MS medium containing 10 mg/1 of 2ip produces highest percentage of bud development and shoot growth.
Adding MS hormone free liquid medium on surface of establishment medium with initiated bud was caused in vitro shoot elongation. Elongated shoots was used for shoot proliferation by permanent cutting of shoot in collar and transferring of them to rooting or shoot elongation media. Highest root induction was observed in MS medium supplemented with 10 mg/l 2iP and 0.01 mg/l IAA ( more than 80%).

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References

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