Genetic evaluation for seed and forage yield in red clover (Trifolium pratense l.) populations through multivariate analysis

Document Type : Research Paper

Authors

1 Science Board of Research Institute of Forests and Rangelands,

2 Research Institute of Forestes and Ranglands, Tehran

3 Science Board of Research Institute of Forests and Rangelands

Abstract

In order to study the genetic variation for forage and seed yields and their components among 9 genotypes of red clover (Trifolium pratnse L.), a complete randomised block design with three replications was conducted under spaced plant condition over two consecutive years in Karaj, Iran. Data were collected for leaf lamina area, internodes length, petiole length, canopy height, persistency, number of inflorescences per stem, number of inflorescences per plant, number of stems per plant, number of floret per head, number of seeds per head, thousand seed weight, growth habit, seed yield and dry matter yield. The data were subjected to analysis of variance for each year and combined over the two years. The data were also analyzed using principal components and cluster analysis.
The results showed significant differences between genotypes for all the traits except internodes length and Inflorence number per plant at 0.01 and 0.05 level. Genotypes No. 324 with average values of 10 ton h-1 and 432 Kg h-1produced higher forage dry matter and seed yield respectively, than other genotypes.
Using principal components analysis, the most important variables for dry matter yield and seed yield were identified. First three independent components accounted for 81% of total variation. The first principal component indicated that forage dry matter yield, internodes length, canopy height, inflorence per plant and persistency were important characters for classification with 55% of total variation. Seed yield, inflorence per stem, seed per inflorence and thousand grain weight were important characters in the second component. In the third component, inflorescence per stem, floret per head and growth habit were determined. Based on the results, it was suggested that components 1,2 and 3 could be known as forage yield, seed yield, and growth habit components, respectively. Based on Ward cluster analysis, entries were divided into 3 groups. Genotypes in cluster number 1 (1753, 4 and 618) averaged well above the overall mean for seed yield and seed components. Genotypes in cluster number 2 (324 and 1568) that were exotic germplasm, had higher values for dry matter yield and its components. Genotypes in cluster 3 were poor for both seed and forage production.

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References

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