Genetic variation among perennial Trigonella by seed storage proteins electrophoresis

Document Type : Research Paper

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Abstract

Genetic variation is the base of plant breeding so that plant selection with desirable characters be possible. One of the methods for determination of genetic variation among the plant species is the use of electrophoresis method. In this research for evaluation of genetic variation between perennial Trigonella, seed samples of six accessions from Natural Resources Gene Bank, Research Institute of Forests and Rangeland and two accessions from Fars province were under investigation.
Electrophoresis of seed storage proteins was carried out by SDS-PAGE method. In this experiment, 16 different bands were observed and according to the results, banding patterns in some multitude were similar and others were completely different. Most bands belonged to genotypes 770 and 7271 and the lowest bands belonged to genotypes 120 and 460. The accessions with the same banding patterns, located in a same group in cluster analysis. The similarity between accessions was 29.29 percent.
 

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