Evaluation of viability changes and dormancy breaking in the seed of same species in Natural Resources Gene Bank

Document Type : Research Paper

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Abstract

To evaluate the germination and dormancy breaking of seeds in Natural Resources Gene Bank, regard to ecological condition of their habitat, many specific treatments were used. The treatments included prechilling (30, 45 and 60 days) on two beds (sand and filter paper), chemical scarification with sulfuric acid (10-60 min) and hot water (70-80 ˚C). In addition, the effects of container material, seed coats and seed origin on viability of Medicago Sativa maintained in uncontrolled storage was investigated. The results showed that the optimum condition similar to habitat condition must be provided, in order to overcome seed dormancy. In most species with intermediate origin, the prechilling was the effective factor for breaking seed dormancy. The sand bed was more suitable for germination of large seeds. To conquer dormancy in tropical seed and seed of legume family, the hot water and sulfuric acid were better treatments respectively. In Medicago sativa, the type of container material (hemp and nylon) affected seed viability, so that after 9 years, the seed lot in hemp container had a higher viability than which contained by nylon. The results showed that decreasing viability in the un-coated seed of M. truncatulla was greater than the coated seed. Therefore, the rate of deterioration of the coated seed and the un-coated seed were 1.49% and 3.57%, respectively.

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References

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