Evaluation of seed yield, forage yield and morphological traits in white clover (Trifolium repens L.) using multivariate analysis

Document Type : Research Paper

Authors

Research Institute of Forests and Rangelands

Abstract

In order to study genetic variation on forage and seed yields and their components among 12 varieties of white clover (Trifolium repens L.), a randomized complete blocks design with three replications was conducted over two consecutive years in Karaj, Iran. The data were collected for leaflet size, internode length, petiole length, canopy height, stolon density, internode thickness, persistency, head density, head per stolon, floret per head, seeds per floret, 1000 seed weight, seed yield and dry matter yield. Results showed significant differences between the genotypes for all of the traits except for head density and thousand seed weight. The varieties of Alice, Huia, Chiftan and Aran with average values of 14.2, 12.8, 12.2 and 11.8 tha-1 produced higher forage dry matter yield. For seed yield Alice and Mikanova with average values of 148.7 and 122.4 kg h-1 had the highest seed production per year, respectively. The results of correlation analysis showed positive and significant relationships between forage yield and canopy height, leaflet size, stolon density, petiole length and persistency. Seed yield had positive relationships with both 1000 seed weight and floret per head.
Using principal components analysis, the first three independent components accounted for 80% of the total variation. The first principal component indicated that forage dry matter yield, canopy height, head per stolon, petiole length and persistency were the important characters for classification with 52% of the total variation. Seed yield, floret per head and thousand grain weight were the enetial characters in the second component. Based on the results, it was suggested that components 1 and 2 could be known as forage yield and seed yield. Based on Ward Cluster Analysis, entries were divided into 3 groups. Accessions in cluster 1 (Huia, Chiftan and Aran) and cluster 2 (Alice, Mikanova and Avoca) averaged well above the overall mean for forage dry matter and seed yield, respectively.

Keywords


1- پیمانی‌فرد، ب.، ملک پور‏ ب. و فائزی پور‏‏‏، م.، 1373. معرفی گیاهان مهم مرتعی و راهنمای کشت آنها برای مناطق مختلف ایران. نشریه شماره 24 موسسه تحقیقات جنگلها و مراتع، تهران. ایران.
2- Annicchiarico, P., Piano, E., and Rhodes, I., 1999. Heritability of, and genetic correlations among, forage and seed yield traits in Ladino white clover. Plant Breeding. 118: 341-346.
3- Collins, R.P., Abberton, M.T., Michaelson-Yeates, T.P.T. and Rhodes, I., 1997. Response to divergent selection for stolon characters in white clover (Trifolium repens ). Journal of Agricultural Science. 129: 279-285.
4- Finne, M.A., Rognli, O.A. and Schjelderup, I., 2000. Genetic variation in a Norwegian germplasm collection of white clover (Trifolium repens L.). 3. Correlation and path coefficient analyses of agronomic characters. Euphytica. 112: 57-68.
5- Humphreys, M.O. 1991. A genetic approach to the multivariate differentiation of perennial ryegrass (Lolium perenne L.) populations. Heredity, 66: 437-443.
6- Jahufer, M.Z.Z., Cooper, M. and Brien, L.A., 1994. Genotypic variation for stolon and other morphological attributes of white clover (Trifolium repens L.). Populations and their influence on herbage yield in the summer rainfall region of New South Wales. Australian Journal of Agricultural Research. 45: 703-720.
7- Moussavi, M., 1979. List of plants of Evin Herbarium, Family: Leguminosae (Genus: Trifolium). Iranian Agricultural and Natural Resource Organization, Plant Pest and Disease Research Institute, Publication Tehran, Iran. No. 14, pages, 50.
8- Olimos, F., Wilman, D. and Hamilton, R.S., 2003. Variation between and within Trifolium repens L. populations collected from sown swards in Uruguay. Euphytica. 130: 131-141.
9- Pederson, C.A., 1995. With clover and other perennial clovers. In: “Forages” (Eds. Barnes, R. F., D.A. Miller and C. J. Nelson), Iowa State University Press, Iowa, USA, pages 227-236.
10- Peters J.P and Martinelli, J.A., 1989. Hierarchical cluster analysis as a tool manages variation in germplasm collections. Theoretical and Applied Genetics 78: 42-48.
11- Piano, E. and Annicchiarico, P., 1995. Persistence of Landio white clover ecotypes and its relationship with other agronomic traits. Grass and Forage Science: 50: 195-198.

12- Rhodes, I., 1987. Characterization of white clover. In: "Collection, Characterizationand utilization of genetics of temperate forage grasses and legumes"(Eds. Tyler, B.F.). International Board for Plant Genetics Resources (IBPGR), Rome, Italy.