Study of drought tolerance improvement in alfalfa (Medicago sativa L.) using somaclonal variation

Document Type : Research Paper

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Abstract

Drought stress in plants in arid and semi arid zones is a normal phenomenon. Invitro selection using tissue culture can help to determine and evaluate the tolerant genotypes. It could be used as a complementary of a classical breeding method for producing, maintaining and applying of germplasm. In this experiment, Yazdi seeds which was selected previously using in vivo selection for drought tolerance, was used. Assessment and selection of calli in a completely randomized design (CRD) with control and different concentration of polyethylene glycol (PEG) 30, 60, 90, 120 and 150 gr/lit, (10 replication in each treatment) was carried out. In the next step, the rate of regeneration and invitro selection is evaluated for drought tolerance with 40 replication in each treatment. In hydroponic experiment, plant selection for drought tolerance was done in - 12bar potential. The results of invitro selection showed that low concentration of PEG, has not significant effect on callus production. There was no significant difference on seed germination and plantlets growth, in population derived from different seeds under PEG stress.
 

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References

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