Seed dormancy breakage of Fagus orientalis Lipsky using embryo culture

Document Type : Research Paper

Authors

Research Institute of Forests and Rangelands P.O. Box 13185-116 Tehran-Iran.

Abstract

Oriental beech (Fagus orientalis Lipsky) is a timber producing species in Hyrcanian forests in north part of Iran. In order to increase seed germination capacity and seedling production of this species, in vitro embryo culture was carried out. cold (four-week cold stratification) and non-cold treated seeds were disinfected. Endocarps of seeds were removed and isolated embryos transferred onto MS and  1  MS media. The results indicated that there is no
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 significant difference between cold treated (4º C) and control seeds for embryo germination at p<0.05 level (72.33 and 77.5% respectively). Therefore, removal of endocarp layer from seed and in vitro embryo culture without cold treatment can cause breakage of seed dormancy. There
was  no  significant differenc e between  MS and  1  MS medium for embryo
                                                                                2
 germination at 0.05 level (78/37 and 72/72% respectively). Produced seedlings were successfully acclimatized and transferred to the greenhouse.

Keywords

References

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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 12, Issue 3 - Serial Number 17
September 2005
Pages 257-264

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