Evaluation of effects of different sources of nitrate on absorbance of N, P, K, chlorophyll content and shoot proliferation of Jojoba Simmondsia chinensis (Link) Sch. in vitro

Document Type : Research Paper

Authors

Research Assistant Professor - Horticulture Department, seed and Plant Improvement Institute. Karaj, Iran.

Abstract

Jojoba is dioecious, evergreen shrub. Seeds of jojoba have special liquid wax and there are many general uses for jojoba oil and is currently used in many different industries. The plant is drought resistant and to some extent also salt resistant. Jojoba may be propagated in vitro. High frequency mass micropropagation of jojoba is very important.
The importance of mineral elements on plant growth is different in plant species and depends on plant genotype and constitution of medium.
Effects of five different concentrations of MS medium and various sources of N on micropropagation of Jojoba were investigated.
The apical and axillary buds of Jojoba were aseptically grown on Murashige and skoog (MS) medium for 30 days. Then, they were transferred on the treatment media and left under controlled environment with day length of 18 hours and temperature of 25° C. NH4NO3 (N1), KNO3 (N2) and NH4NO3 plus KNO3 (N3) with different concentrations (1.4MS, 1.2MS MS, 0.8MS, 0.6MS ) used as nitrogen sources in the media.
An increase in shoot proliferation was observed after 30 days of transplanting to the treatments media N1 and N3 (concentration of 0.8 N compared to MS as control and 45 days after transplanting were observed in N1 medium (concentration of 1.4 , 1.2, 0.6 N1 compared to MS as 1) and N3 media (concentration of 0.6 compared to MS as 1).
 

Keywords

Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 12, Issue 2 - Serial Number 18
October 2005
Pages 433-446

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