Effects of gibberellic and citric acid on germination percentage, speed of germination and seed vigor of Eremurus spectabilis M.B.

Document Type : Research Paper

Authors

1 M.S. Student of Azade Islamic Un. Po Box.13185-116

2 Azade Islamic Un. Tajrish Sq. Darband street Partovy avenue

Abstract

In order to study seed germination characterisitics of Eremurus spectabilis under physical and chemical treatments, the mature seeds of Eremurus spectabilis were treated by collected from National Botanical Garden of Iran. Seeds were imposed under physical stimulator pretreatments, including soaking of seeds in water for 24-48h, cutting of seeds’ tip, making abrasion on seed cortex, exposing to light for 24h, 12h and incubating in darkness. It was also followed by chemically stimulation the seeds, using sodium hypochloride, citric acid and gibberellic acid in different constrations, in order to achieve suitable methods of germination and breaking dormancy. Soaking of seeds in the water for 24-48h, removing seed cortex and washing in 35% sodium hypocholoride and distilled water, cutting seed tip and treat in gibberrelic acid (0.01 M.) and citric acid (50 mg/lit) for 45 minutes and temperature 10-15°C and white light, 24h (4500-5000 lux) for 1-3 weeks led to the highest germination percentage (53.3%), germination speed (0.88) and seed vigor (10.2).

Keywords


سرمدنیا، غ.، 1375. تکنولوژی بذر. انتشارات جهاد دانشگاهی مشهد. مشهد. 288 صفحه.
علیزاده، م.، عیسوند، ح.، 1380. بررسی درصد جوانه‌زنی، سرعت تجمعی جوانه‌زنی و شاخص بنیه بذر 9 گونه دارویی تحت شرایط آزمایشگاهی مطلوب و پیش سرما. انتشارات مؤسسه تحقیقات جنگلها ومراتع. تهران. جلد شماره 7. شماره انتشار 279. صفحات 133 تا 146.
وندلبو، پر. 1355. لاله‌ها و زنبقهای ایران. انتشارات مؤسسه تحقیقات جنگلها و مراتع. تهران. 88 صفحه.
 
Chase, M.W., Stuart, P.A.  and Ilia, J., 2000. Phylogenetics of Asphodelaceae (Asparagales): An  Analysis of Plastid rbcl and trnl-F DNA sequences, Annals of Botany, UK. and Centro De Ecologia . No. 86, pp. 935-951.
Doussi M.A. and Thanos C.A., 2002. Ecophysiology of seed germination in Mediterranean geophytes Muscari spp. Seed Science Research, Athens, Greece. Vol.12, no.3, pp. 193-200.
Ellis, R.H., Hong. T.d., and Roberts, E.H., 1985. Handbook of seed Tecnology for Genebanks. Rome, International Board for Plant Gentic Resources, Vol. 1,2.
Macedo, C.E., Kinet, J.M and Lutts, S., 2001. Aluminum effects on Citric and Malic Acid excretion in roots and calli of Rice Cultivars, R.Bras. Fisiol. Veg.,131:13-23.
Rechinger, K.H., 1992. Akademisch Druk- U.Verlagsastalt, Graz. Printed in Austria . Vol.Liliaceae (151/1, 165/2, 170/3).
Tian, X., Knapp,A.D., Gibson, L.R. and Struther, R., 2003. Seed Physiology, Production and Technology Response of Eastern Gamagrass seed to Gibberellic Acid Buffered below Its PKa. Crop Science , Low Agriculture Journal No. J-18414. 43: 927- 933. Project No. 3244.
10. Vargas, M.E., 1986. Efecto del Acido Giberelico, 6- Bencil – amino  Purina  y esttriticacion sobre la germination de semillas de chirimoyo (Annona cherimola Mill.)cv. Bronceada. Tesis – Universitad Catolica de Valparaiso, Quillota, Chile.