Micropropagation of Acer cinerasens by shoot tip culture

Document Type : Research Paper

Author

-Research Institute of Forests and Rangelands. P.O.Box 13185-116, Tehran, Iran.

Abstract

Acer cinerasens is the most important and useful species of Aceraceae among plants in the Zagrose ecotypes. Regarding difficulties in conventional propagation of hardwood plants specially hard rooting of cuttings from adult trees of Acer, this study was carried out. To investigate shoot tip culture of Acer cinerasens, apical buds and micro cuttings were gathered in different seasons from selected mature trees at Ghallajeh forest in Kermanshah and Sarchahan forests in Shiraz (Gen 1, Gen 2). After different sterilizing treatments, they were cultured on MS (N/4) or DKW media. MS (N/4) medium containing 0.5 mgl-1 2ip, 0.1 mg/l Kinetin, 0.05 mg/l TDZ, 0.25 mg/l GA + 0.01 mgl-1 IBA was the best treatment for shoot proliferation and elongation. The shoots were treated as cuttings by different rooting treatments but it was not successful.

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References

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