Propogation of Populus caspica tree through mature ovary culture

Document Type : Research Paper

Author

Research Institute of Forests and Rangelands, P.O.Box 13185-116, Tehran, Iran.

Abstract

In order to mass propagation of Populus caspica, in vitro matured ovary culture was used. For embryo germination, 14 days old capsules, derived from natural and artificial (twig and pot pollination system) pollination were isolated. Isolated capsules were then transferred to MS, Half-MS (Half-concentrated MS) and SS (solidified 3% sucrose solution) hormone free medium for embryo germination. Significant differences (p<0.01) were observed between media and two kind of pollination for mean number of plantlets indication per isolated capsule. High number of plantlets was observed per capsule in half- MS medium. The artificial pollination of catkin showed higher number of plantlets production per capsule than that of the occurring natural pollination (24.77 and 19.46 respectively). Plantlets with 5 or 8 cm height were then cultured in the same medium within jars, before being transferred to the potting soil. More than one thousand plantlets were produced from both kind of pollination. Successfully acclimatized plantlets in green-house were then transplanted to the field.

Keywords

References

  1. ثابتی، ح.، 1354. صنوبر در ایران. انتشارات سازمان تحقیقات کشاورزی و منابع طبیعی.
  2. ثابتی، ح.، 1355. درختان و درختچه‌های ایران. انتشارات سازمان تحقیقات کشاورزی و منابع طبیعی.
  3. جلیلوند، ح.، 1367. بررسی انتشار جغرافیایی و شرایط اکولوژیکی گونه سفید پلت در جنگلهای شمال، پایان نامه کارشناسی ارشد  دانشگاه تربیت مدرس دانشگاه مازندران.
    1. Gaget, M., Said, C., Dumas, C. and Knox, R.B., 1984. Pollen Pistil interaction in interspecific crosses of Populus (section Aigerios and Leuce), pollen adhesion hydration and cellose responses. J. Cell Sci. 72:184-192.
    2. Murashige, T. and Skoog, F., 1962. A revised medium for rapid growth and bioassays with Tobacco tissue cultures. Physiol. Plant. 15:473-479.
    3. Noh, E.R., Kao, Y. B. and Lee, S. K., 1986. Hybridization between incompatible poplar Species through ovary and embryo culture. Res. Rep. Inst. For. Genet. 22:9-14.
    4. Savka, M. A., Dawson, J. O., Jokela, J. J. and Skirvin, R. M., 1987. A liquid culture method for rescuing immature embryos of eastern cottonwood. Plant Cell Tissue Organ Cult. 10: 221-226 1987.
    5. Stettler, R.F., 1980. Interspecific crossability studies in poplars. Theor. Appl. Genet. 58:273-282.
    6. Wright, J.W., 1976. Introduction to forest genetic. Academic Press New York, pp: 463.
  1. Driver, J.A. and Kuniyuki, H., 1978. In vitro propagation of paradox walnut root stocks (Jugland hindsii x J.regia). Hortscience 19:507-509.
  2. Eaton, G.W. and Jamont, A.M., 1992. Embryo sac development in the apricot, Prunus armeniaca L. cv. Constan. Journal of Horticultural Science 86: 95-101.
  3. Furokawa, Y. and Bukovac, M.J.1989. Embryo sac development in sour cherry during the pollination period as related to fruit set..Hort Science 24: 1005-1013.
  1. Guries, R.P. and Stettler, R.F., 1976.  Pre-Fertilization Barriers to hybridization in the poplars. Silvae Genetic 25:37-44.
  2. Heslop Harrrison, J.: Incompatibility and the Pollen-Stigma interaction. A. Rev. PL. Physiol 1975
  3. Jafari, M.A., Modir-rahmati, A., Tavesoli, A., 1998. Application of ovary and ovule culture in P. albaL x Populus euphraticaOLIV hybridization. Silvae Genet. 47:5-6.
  4. Jafari, M. A., and Modir-rahmati, A., 2000. Production of Populus euphratica OLIV. x P. alba L. hybrid poplars through ovary and ovule cultures. Plant Genetic Newsletter. 122:13-15.
  5. Jalili, A. and Jamzad, Z., 1999. Red data book of Iran. Pp. 748.
  6. Knox, R. B., Willing, R. R. and Pryor, L. D., 1972.  Interspecific Hybridization of poplars using recognition pollen. Silva Genet. 21:125-128.
  7. Kouider, M., Skirvin, R. and   Saladin, K. P., 1984. A method to culture immature embryo  of Populus deltoides  in vitro. Can. J. For . Res 14:956-958.
  8. Li, W. and Li, J., 1985. In vitro culture of Hybrid ovules in populus. Sci. Silv. Sin. 21:339-346.
  9. Li, W., Wang, R. and Zhu, X., 1983. On the embryo development and ovule culture of interspecific hybrids between Populus simonii and P.pyrasrmidalis Borkh.  Acta  Bot. Sin. 25: 409-417.
  1. Raquin, C. and Troussard, L., 1993. In-ovary embryo culture as tool for poplar hybridization. Can. J. Bot.  . 71: 1271-1275.
  1. Schreiner, E., 1965. Maximum Genetic improvement of fores trees through synthetic multiclonal hybrid varieties, Proc. 13th Northeast Forest tree Impr. Conf., Albany N. Y., pp.7-13.

24. Zobel, B.J., 1984. Applied forest tree improvement. John Wiely and Sons New York pp: 505.

Files

Share

How to cite

Statistics