Investigation of genetic variation and taxonomic relationships of Lolium species using seed storage proteins

Document Type : Research Paper

Authors

1 Imam Khomeini Higher Education center

2 Reasearch Institute of Forests and Rangelands, Tehran, Iran

Abstract

The genus Lolium belongs to Poaceae (Festuceae). It has natural distribution in Iran and found in rangelands of North and West parts of the country. This study was performed in order to investigate genetic variation and taxonomic relationships of the Lolium species in Iran. Seeds of 18 populations, which collected from different parts of the country or received from genebanks of research institutes, were used in this study. First, 0.2 g of bare seeds were ground and their proteins were extracted. Extractions were run on polyacrylamid gel by SDS – PAGE methods. Twenty-one bands were detected. Each band was considered as a taxonomic character. The data (population × band number) were analyzed, using factor and cluster analyses. Results showed that species of the genus were distinct and isolated from each other. Inbreeding species were, also, isolated from outbreeding.

Keywords

Main Subjects

References

میرجلیلی س. ع.، 1381، بررسی تاکسونومیکی و سیتوتاکسونومیکی جنس Lolium در ایران، رساله دوره دکتری علوم گیاهی گرایش سیستماتیک گیاهی. دانشکده علوم، دانشگاه اصفهان.
میرزایی ندوشن، ح. و ندرخانی، ه.، 1379. مطالعه کاریوتیپی جمعیت‌های تتراپلویید لولیوم. تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران:4.
Balfourier, F., Charmet, G. and Ravel, C. 1998. Genetic differentiation within and between natural populations of perennial and annual ryegrass (Lolium perenne and L. rigidum). Heredity, 81: 100-110.
Bennett, J. S. 1997. A phenetic analysis and lateral key of the genus Lolium (Gramineae), Genetic Resources and Crop Evolution, 44 : 63-72.
Bennett, S. J. and Hayward D. 1999. Electrophoretic differentiation in isolated populations of Lolium rigidum Gaud. Molecular Ecology 8: 123 -131.
Bennett, J. S. 2000. Morphological differentiation in four species of the genus Lolium. Genetic Resources and Crop Evolution, 47: 247 - 255.
Bennett, S. J. 1994. An ecogeographical study of the genus Lolium in Europe. PhD Thesis. University of Birmingham.
Bor, N. L. 1970. Gramineae, In: Rechinger K. H. (eds.) Flora Iranica, 70: 150-184. Akademische Druck-und verlagsansalalt-Gras, Austria.
Bor, N. L. 1968. Gramineae, in: Tounsend C.C., Guest E. and Al-Rawi A.(eds) Flora of  Iraq, 9:208-224. Ministry of Agriculture. Republic of Iraq.
Bulinska – Radomska, Z. and Lester, R. N. 1985. Relationships between five species of Lolium (Poaceae). Plant Systematic and Evolution, 148: 169-175.
Charmet, G., Balfourier, F. 1994. Isozyme variation and species relationship in the genus Lolium L.(ryegrass,Gramineae). Theoretical and Applied Genetics. 87: 641-649.
Chen - Lixue, Fischer H., Jensen U. and Chen - Lx 1996. Accumulation of seed storage proteinsand the taxonomy of poaceae. Plant Systematics and Evolution 206: 1 - 4, 243 - 257.
Clapham, A. R., Tutin, T. G. and Moor, D. M. 1978. Flora of the British Isles, Cambridge University Press, Cambridge.
Emoto, T. 1989. Taxonomic studies of Festuca and Lolium based on isozyme variation. Bulletin of the Akita Prefectural - College of Agriculture. 15, 75-109.
Ferguson, J.M. and Grabe D.F. 1984. Separation of annual and perennial species of ryegrass by gel electrophoresis of seed proteins. Journal of Seed Technology. 9: 137-149.
Ferguson, J. M. and Grabe, D. F. 1986. Identification of cultivars of perennial ryegrass by SDS-PAGE of seed proteins. Crop Sciense. 26: 170-176.
Hubbard, C. E. 1954. Grasses, Penguin Books Ltd, Middlesex.
Humphries, C. J. 1980. ‎Lolium ‎ L.In: T.G. Tutin, V.H. Heyward, N.A. Burgess, D.M. Moore, D.H. Valentins, S.M. Walters and D.A. Webb (Eds), Flora Europea Vol. 5, Alismataceae to Orchidaceae (monocotyledons), pp. 163-154. Cambridge University Press. Cambridge.
Loos, B. P. 1993a. Morphological variation in Lolium (poaceae) as a measurment of species relationships. Plant Systematics and Evolution, 188: 87-99.
Loos, B. P. 1993b. Allozyme variation within and between populations in Lolium (Poaceae). Plant Systematic and Evolution. 188: 101 - 113.
Mill, R. R. 1985. Lolium L. In: P. H. Davis (Ed.), Flora of Turkey and the East Aegean Islands, University Press, Edinburgh.
Murphy, P.M., Culleton, N. and Flaherty, T. 1990. Identification of grass seed cultivars by SDS polyacrylamid gel electrophoresis. Irish Journal of Agriculture Research. 29: 2, 117-127.
Parsa, A. 1950. Flore de l'Iran. vol:V. Publication Du Ministere De l' Education. Museum D'Historie Naturele De Tehran.
Prat, H. 1960. The cytology of certain intergeneric hybrids between Festuca and Lolium. J. Genetics, 28: 113 - 156.
Statsoft, Inc. 1995. Statistica for windows (User Guide), Tulsa, OK, USA.
Terrell, E, E., 1968. A taxonomic revision of the genus Lolium. Technical Bulletin of theUnited States Department of Agriculture, 1392.
 
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 13, Issue 3 - Serial Number 21
October 2006
Pages 257-270

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