Evaluation of ionic equilibrium and propagation coefficient of jojoba (Simmondsia chinensis (link) Sch.) in vitro

Document Type : Research Paper

Authors

1 - Department of biology, Faculty of science, Tehran University

2 Seed and Plant Improvement Institute (SPII)

3 Department of biology, Faculty of science, Tehran University

4 Research Institute of Forests and Rangelands

Abstract

Correlation between media and explants in micropropagation method in different stages of growth and absorption, transport and collection of materials are unknown. Different species of plants that grown in the same conditions differ in use of salts. The form of N uptake is also determined by plant preferences for certain N forms. The form of N uptake is also subject to plant preferences by which plants maintain their cation/anion balance during uptake. Ion equilibrium and propagation coefficient of jojoba were analyzed on the treatment media (different sources and concentrations of N compared to MS medium). The explants grown in MS medium containing 0.5 mgl-1 BA and 0.01 mg l-1 IAA for 30 days. The explants were transferred to treatment media (NH4NO3 ¸ KNO3 andKNO3 + NH4NO3). They were left under controlled environment with temperature of 25 °C and day length of 18 hours. We analyzed content of N, P, K, Na, Ca, propagation coefficient and dry weight of samples. The highest content of mineral elements was in samples from media including NH4NO3 or KNO3 + NH4NO3. Contents of K and Na in samples showed no significant difference. An increase in ion content, dry weight and propagation coefficient was observed after 45 days of transplanting to the treatment media including NH4NO3.

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References

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