Breaking dormancy and germination of wild pear (Pyrus spp) seeds

Document Type : Research Paper

Authors

1 Azad University, Jahrom Branch

2 Research Center for Agriculture and Natural Resources of Fars Province

Abstract

Several species of wild pear are native to Fars province in Iran, but little information about different aspects of their growth habit is available.  Investigation on breaking seed dormancy and germination is needed for propagation of wild pear species as a rootstock for common pear and for afforestation. This research was carried out to study the methods of breaking dormancy, germination and seedling growth of different genotypes of three wild pear species including: Pyrus syriaca, Pyrus glabra and a probable natural hybrid of Pyrus syriaca ´ Pyrus glabra.  Seeds of Pyrus glabra (genotype 11) were used for test of viability, excised embryos and chemical scarification.  Seed viability was tested using tetrazolium (1%) and results showed that seeds were 100% viable. Based on the results of excised embryo test, germination percentage of stratified excised embryos was more than that of non stratified embryos.  Chemical scarification with concentrated sulfuric acid did not affect breaking dormancy of seeds. Results of seed stratification for 0, 15, 30, 45 and 60 days indicated that 60 days stratification was the best treatment for removing dormancy in wild pear species seeds. Seeds originated from different trees (each tree considered as a genotype) of wild pear species showed different responses with regard to stratification period.  Seedling growth of Pyrus glabra was affected by stratification period and 45 days stratification was more effective on hypocotyl length and seedling fresh weight than the other treatments.  It could be concluded that stratification for 60 days was the best treatment for breaking dormancy and seed germination of wild pear species studied in this research.

Keywords


حمزه‌پور، م. و بردبار، ک.، 1381. بررسی برخی از خصوصیات کمی و کیفی تیپ گلابی‌وحشی-شن در منطقه سپیدان. پژوهش و سازندگی، شماره 56 و 57: 47-41.
خاتم ساز، م.، 1371. فلور ایران، شماره 6 :تیره گل سرخ(Rosaceae) ، انتشارات مؤسسه تحقیقات جنگلها و مراتع. چاپ اول. 352 ص.
 
Ann., 1993. Rules for testing seeds. J. Seed Tech. 16(3):1-11.
Benson, D. A., 1970. Data Field. USDA Forest Service, Eastern Tree Seed Lab., Macon, Ga.
Del Monte, J. P., and Tarquis, A. M., 1997. The role of temperature in the seed germination of two species of the Solanum nigrum Complex. J. Exp. Bot. 48:2087-2093.
Dhillon, B. S., and Sharma, M. R., 1978. Note on the effect of growth regulators on germination of wild Pyrus seeds. Ind. J. Agri. Sci. 48: 370-372.
Djvanshir, K. and Pourbeik, H., 1976. Germination value. A new formula. Silvae Genet. 25:79-83.
Felmion, F. and Poole, H., 1948. Seed viability tests with 2, 3, 5-triphenyl-tetrazolium chloride. Contrib. Boyce. Thompson. Inst. 15: 243-25.
Hartman, H. T., 1967. Effect of various treatments on seed germination of several tree species. Proc. Plant Propag. Soc. 8: 126-35.
Hummer, K. E. and Postman, J. D., 1974. Pyrus L. in: Schopmeyer, C. S. (Tech.coordinator),.Seed of woody plant in the United States. Washington, Forest Service, USDA, 883p.
Kabar, K., 1998. Comparative effects of kinetin, benzyladenine and gibberellic acid on abscisic acid inhibited seed germination and seedling growth of red pine and arbor vitae. Turk. J. Bot. 22:1-6.
Khan, A. A., 1971. Cytokinins permissive roles in seed germination.  Science. 171: 833-839.
Khan, A., 1982. Gibberellin and seed development. 110-135.  In: Khan, A (ed.). The Phisiology and Biochemistry of seed Development, Dormancy and Germination. Elsevier Biomedical Press, New Yourk, U. S. A.
Martin, G. C., and Nishijima, C., 1979. Abscisic acid in pear seed, fruit and fruit exudate. J. Amer. Soc. Hort. Sci. 104:185-188.
Schmidt, L. 2000. Guide to handling of tropical and sub-tropical forest seed. Danida Forest seed center. 511p.
Seely, S. D., and Damavandy, H., 1985. Response of seed of seven deciduous fruit to stratification temperature and implications for modeling. J. Amer. Soc. Hort. Sci. 110:726-729.
Straus, S. D., 1969. A study of the physiology of dormancy in the genus Pyrus. Doctoral thesis, Oregon State University.
Taylor , J. S. and Wearing, P. E., 1979. The effect of stratification on the endogenous levels of gibberellins and cytokinins in seeds of douglas fir (Pseudotsuga menziesii Mirb Franco.) and sugar pine (Pinus ambertiana Doug). Plant Cell Environ. 2:165-172.
Westwood, N. N., and Bjornstad, H.O., 1968. Chilling requirements of dormant seeds on 14 pear species as related to their climatic adaptation. Proceedings of the American Society of Horticultural Science 92:141-149