Regeneration of Acer cinerasens through Embryo culture

Document Type : Research Paper

Authors

1 Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran. E-mail: memam@rifr-ac.ir

2 Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran.

3 Research Center of Agricultural and Natural Resources of Kermanshah Province, Iran.

4 Research Center of Agricultural and Natural Resources of Fars Province, Iran.

Abstract

Acer cinerasens is the most important and useful species of Aceraceae. Regarding difficulties in conventional propagation of hardwood plants, specially rooting of cutting in adult Acer trees, this study was carried out. To investigate in vitro culture of Acer cinerasen, seeds were gathered in different seasons from selected mature trees at Sarchahan forest in Shiraz. For culture of mature embryos, seeds were washed with running tap water for 2 days and then surface sterilized with 0.1 % (W/V) mercuric chloride. The embryos were aseptically dissected and transferred in a modified MS medium. Seedling emergence after 4 weeks ranged from 35%. After acclimatization, seedlings were transferred to soil under green house condition. Regarding difficulties in conventional propagation of Acer, specially Acer cinerasens, this study led to production of plants through embryo culture that will be important.

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References

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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 14, Issue 3 - Serial Number 25
November 2006
Pages 170-174

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