Optimizing callus culture in Zataria multiflora boiss for rosmarinic acid production

Document Type : Research Paper

Authors

1 Shahid Beheshti University, Dept of biology, Tehran

2 Shahid Beheshti University, Dept of biology, Tehran, Iran

Abstract

Optimization of callus culture of Zataria multiflora carried out in order to improve rosmarinic acid (RA) production as a secondary metabolite.  The research was conducted using factorial experiments based on randomized complete design. Callus induction from the shoot node culture was realized on MS medium supplemented with 2,4-D (0.5mg/l) and Kinetin (1mg/l). Optimized growth was obtained on Gamborg medium (salt and vitamins) with BA (0.75 mg/l) in dark. The tissues were cultivated in the same medium with sucrose (30, 60 g/l) or glucose (60, 75, 90 g/l) under 16h light/ 8h dark or continuous dark. Accumulation of RA was measured after 45 days of culture by HPLC. Accumulation of RA in callus culture was higher than in seedlings. Also, the higher biomass was obtained with callus culture. High quantities of RA (15.6%) were obtained with glucose treatment. There was not significant difference between 90 and 75g/l glucose for RA content, but higher callus growth was obtained with 75g/l than with 90g/l glucose. The results indicated that osmotic agents and illumination had a significant effect on RA accumulation in Zataria callus cultures. Furthermore, higher biomass and RA amounts can be obtained from callus cultures than from the cultured seedling.

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References

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