Micropropagation of Eucalyptus gongylocarpa Blakely

Document Type : Research Paper

Authors

1 Payame Noor, Biology of Plant Sciences

2 Research Institute of Forests and Rangelands, P.O. Box: 13185-116, Tehran, Iran

3 Gorgan Azad Islamic University, Gorgan, Iran

Abstract

Micropropagation of E. gongylocarpa Blakely through bud culture was investigated. Different treatments of sterilization, establishment, shooting and rooting were studied. The best sterilization treatment for seeds was 0.5% Benomyl solution for 15 min, washing with detergent and then under running water for two hours, 70% ethanol for 20 seconds and 2% NaOCl for 20 min. MS medium containing half strength of nitrate, 0.1 mgl-1 BAP, 0.2 mgl-1 Kin, 0.01 mgl-1 IBA and 0.25 mgl-1 GA3 with 100 mgl-1 PVP were selected for shoot establishment and multiplication. The best rooting, observed in MS medium including 1 mgl-1 IBA with 2.5 gl-1 activated charcoal. The micropropagated plantlets established in the greenhouse successfully.
 

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References

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