Cytogenetic study on some Hedysarum species available in the Natural Resources Gene Bank of Iran

Document Type : Research Paper

Authors

Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran

Abstract

In order to study of cytogenetic characteristics in Hedysarum, the karyotypes of six populations from four species were analyzed using image analysis system. The basic chromosome number was x=8 in all of the populations, but their ploidy level varied. According to Stebbins categories, all of the populations were grouped in symmetric class of 1A, indicating a symmetric karyotype. Variance analysis showed a significant difference (P<%1) among the genotypes for all of traits. Using principal components analysis, the first two components justified %99.50 of total variance. For the first component, the length of long arm, total length of chromosome, centromer index and the length of short arm with the highest coefficients of eigen vectors, were the most important traits. For the second component, the arm ratio had the most important role for total variation. Cluster analysis classified the genotypes into two groups. The highest distance were observed between H. coronarium (Gorgan) and H. criniferum (Kermanshah) indicating the least affinity between them. The lowest metric distance values were obtained between two accessions of H. kopetdaghi (Solgerd and Sharlogh). According to the dispersion diagram, based on first two components, the genotypes were grouped in two distinct classes, corresponding with the results of cluster analysis. According to the results of cluster analysis based on A1 and A2 parameters, the populations were grouped in two classes. The highest distance was observed between H. criniferum (Zanjan) and H. kopetdaghi (Solgerd), which implied the lowest affinity between them. Lowest distance was observed between H. criniferum (Kermanshah) and H. kopetdaghi (Solgerd).

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References

 
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