Study of callus induction and regeneration in Populus euphratica Oliv. via ovary culture

Document Type : Research Paper

Authors

1 Research Institute of Forest and Rangelands, P.O. Box 13185-116, Tehran, Iran

2 Research Institute of Forest and Rangelands, P.O. Box 13185-116, Tehran, Iran.

3 - Research Institute of Forest and Rangelands, P.O. Box 13185-116, Tehran, Iran

Abstract

Immature and mature ovaries of Populus euphratica Oliv.were harvested from the female trees on the bank of Karun river. Five sterilization treatments were tested in a completely randomized design (CRD) with eight replications. 0.1% mercuric chloride and 70% ethanol both for 30 seconds, 70% ethanol for 30 seconds and 25% sodium hypochlorite for 10 minutes and also 70% ethanol for 1 minute and 25% sodium hypochlorite for 10 minutes were the best treatments. In order to Callus induction, four treatments were evaluated through a covariance analysis in a CRD design with six replications. MS basal medium supplemented with 3 mgl-1 IBA and 0.1 mgl-1 BAP and also 3 mgl-1 2,4-D and 0.1 mgl-1 Kin were proposed for callus induction. In addition, the regression equation between the explants age and the amount of callus production was obtained. Regression curve demonstrated the decrease of callus induction as a result of increase in explants age. Regeneration was studied using four hormonal treatments in a factorial experiment with four replications based on a completely randomized block design. MS basal medium supplemented with 2 mgl-1 BAP and 0.5 mgl-1 NAA was suggested as the best treatment. Also, combination of NAA and IBA both 0.1 mgl-1 in half MS medium was suggested as the best treatments for root production. After the different stages of acclimatization in soil, the plantlets were transferred to field.

Keywords

Main Subjects

References

-     جورابچی، ع.، 1379. تنوع سوماکلونال و گامتوکلونال در گیاهان باززایی شده از کشت سلول و کالوس گیاه صنوبر پده (Oliv. Populus euphratica)، پایان‌نامه کارشناسی ارشد رشتة زیست شناسی سلولی مولکولی، دانشکده زیست شناسی، دانشگاه خاتم.
-     فارسی، م. و ذوالعلی، ج. (چاولا، اچ. اس.)، 1382. اصول بیوتکنولوژی گیاهی، انتشارات دانشگاه فردوسی مشهد. صفحات 43-12.
-     شهرزاد، ش. و امام، م.، 1379. تکثیر غیرجنسی پده (P. euphartica Oliv.) به روش کشت بافت، سمینار ارائه شده در مجموعه مقالات مؤسسه تحقیقات جنگلها و مراتع کشور، شماره 230، صفحات 23-11.
-     محمودی‌کردی، ف.، 1379. کشت بساک و ایجاد گیاه‌ هاپلوئید در صنوبر پده و بررسی مقایسه‌ای تشریحی گیاهان ‌هاپلوئید ایجاد شده با دیپلوئید طبیعی، پایان‌نامه کارشناسی ارشد، دانشکده علوم، دانشگاه تربیت معلم.
-     Ahuja, M.R., 1984. A commercially feasible micropropagation method for aspen. Silvae Genetica, 33:174-176
-     Antonetti, P.L.E. and Pinon, J., 1993. Somaclonal variation within poplars. Plant cell, tissue and organ culture, 35:99-106
-     Hyndman, S.E., Hasegawa, P.M. and Bressan, R.A., 1982. The role of sucrose and nitrogen in adventitious root formation on cultured rose shoots. Plant cell, tissue and organ culture, 1:229-238
-     McNabb, H.S., 1997. Micropropagation, genetic engineering and molecular biology of Populus in: Klopfenstein, N.B.,Y.W. Chun, M.S. Kim and M.R. Ahuja, (eds.).
-     PP. 1-2, Rocky Mountain Forest and Range Experiment Station publisher, Colerado.
-     Pierik, R.L.M. 1997. In vitro culture of higher plants. kluwer. Academic Publishers, Netherlands. , 195-196 pp.
-     Parsons, T.J. and Sinkar, V.P., 1986. Transformation of poplar by Agrobacterium tumefaciens. Bio. Tech, 4:533-536.
-     Redenbaugh, M.K., Westfall, R.O. and Karosky, D.F., 1981. Dihaploid callus production from Ulmus Americana anthers. Bot. Gaz, 142:19-26
-     Shiji, W., Binghao, C. and Hgun, L., 1995. Euphrates poplar forest. China environmental science press, 38-43.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 15, Issue 3 - Serial Number 29
November 2007
Pages 231-242

Files

Share

How to cite

Statistics