Effects of genotype and growth regulators on callus induction in Rosa damascena Mill

Document Type : Research Paper

Authors

1 Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran, E-mail:tabaei@rifr-ac.ir

2 Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran,

3 Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran

Abstract

Rosa damascena Mill. as a most important aromatic plants is the best commercial species of the fragrant roses in Iran and many countries. To study callus induction and proliferation in Rosa damascena Mill., axillary buds of five genotypes collected from different parts of Iran was used as explants. These were cultured on MS medium supplemented with growth regulator(s) and solifiled with 0.7% (w/v) agar, at 25±1 °C. Different concentrations of 2,4-D (2, 3 ,4 ,5 ,6 mg/l), IBA (0.1 mg/l), NAA (5 ,10 mg/l), PCPA (3, 5 ,7 ,9 ,10 mg/l) Kinetin (0.1, 1 mg/l) and BAP(1, 2mg/l) were used. Genotypes showed significant differences (P<0.01) for callus induction, Also, highest callus induction was observed in genotypes of Kashan cultured on MS cultured medium contained 1 mg/l Kinetin and 5 mg/l PCPA. The results emphasized on genotype, kind and concentration of growth regulators as key factors in callogenensis and proliferation of Rosa damascena.

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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 15, Issue 3 - Serial Number 29
November 2007
Pages 222-230

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