Study of chemical composition and nutrition value of prennial lucerne (Medicago sativa L.) and genetic diversity based on SDS-PAGE markers

Document Type : Research Paper

Authors

1 Keramnshah Research Centre of Agriculture and Natural Resources. Kermanshah, Iran

2 Payame Noor University (PNU) and Research Centre of Agriculture and Natural Resources, Kermanshah, Iran

3 Razi Univ. Kermanshah, Iran

Abstract


Genetic diversity is one of the most important criteria to select parents in breeding programs. The research was carried out in order to determine genetic variability among prennial lucerne based on chemical composition, nutrition values and SDS-PAGE traits. 18 lucernce genotypes were cultivated in a randomized complete block design with two replications. Chemical composision, nutrition values and reserved proteins in seeds and leaves were electrophoresed. Based on cluster analysis of SDS-PAGE, 18 genotypes were grouped into 3 clusters Genotypes 13, 14, 15, 16, 17 and 18 in all SDS-PAGE methods were placed in same group, while genotype 12 was located in separate cluster. Principal components analysis revealed that the crud protein, P and K had the most relative variance amount.

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References

-  فضائلی، ح.، 1371. تعیین ترکیبات شیمیایی و انرژی خام منابع خوراکی دام و طیور استان گیلان، پایان‌نامه کارشناسی ارشد، دانشگاه تربیت مدرس.
-  کریمی، ه.، 1369. یونجه. مرکز نشر دانشگاهی، تهران.
-  مصطفایی، ع.، 1382. راهنمای نظری و عملی الکتروفورز پروتئین در ژل، چاپ دوم، انتشارات یادآوران، 174 صفحه.
-  ولیزاده، م.، 1376. استفاده از الکتروفورز پروتئینها در ارزیابی فاصله ژنتیکی گونه‌های یونجه، مجله علوم کشاورزی ایران. جلد 28. شماره 2.
 
Ahmed, M.F., 1994. A study of the cyto- taxonomy for conservation of genetic resources of forage legumes (Medicago species) in Omayed Biosphere Reserve. Scientific Report, University of Alexandria, Egypt. Blackstock, W.P., and M.P. Weir, 1999. Proteomies: quantitative and physical mapping of cellular protein. Trends in Biotechnology, 17: 121-127.
-  Fazli, H. and Yazdisamadi, B., 1992. Comparison of 64 Iranian and foreign alfalfa land- races and cultivars for their agronomic and qualitative characteristics. Iranian Journal of Agricultural Sciences. 23 (1):15
-  Julier, B., Huyghe, C. and Ecalle, C., 2000. Within and Among- Cultivar Genetic Variation in Alfalfa: Forage Quality, Morphology, and Yield. Crop Sci., 40:365-369.
-  Kameswara Rao, N., 2004. Plant genetic resources: Advancing conservation and use through biotechnology.African Journal of Biotechnology, 3(2): 136-145.
-  Krishnan, H.B. and Sleper, D.A., 1997. Identification of tall fescue cultivars by sodium dodecyl sulfate polyacrilamide gel electrophoresis of seed proteins. Crop Sci., 37:215-219.
-  Robins, G.J., Luth, D., Campbell, T.A., Bauchan, G.R., Chunlin He, Viands, D.R., Hansen, J. L. and Brummer, E.C., 2007. Genetic mapping of Biomass production in tetraploid Alfalfa. Crop Science, 47: 1-10.
-  Vitale, M., Pupilli, F., Labombarda, P. and Arcioni, S., 1998. RAPD analysis reveals a low rate of outcrossing in burr medic (Medicago polymorpha L.). Genetic Resources and Crop Evolution, 45: 337-342.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 15, Issue 3 - Serial Number 29
November 2007
Pages 196-210

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