Study of tissue culture of Achillea millefolium L.

Document Type : Research Paper

Authors

Islamic Azad University Iran, North Tehran branch, Biology department, Tehran

Abstract

In this research, in vitro culture of Achillea millefolium L. was investigated with different hormones such as IAA, BA, NAA, Kin and 2,4-D. Different parts of natural plant and seedlings grown on  hormone free medium were used as explants. The best medium for callogenesis was a medium supplemented with 1 mgl-1 NAA and 2 mgl-1 Kin. Apical meristem of seedling in medium supplemented with 1 mgl-1 IAA and 2 mgl-1 BA produced shoots after forming callus. Leaf explant in medium supplemented with 1 mgl-1 IAA and 2 mgl-1 BA and medium supplemented with 2 mgl-1 IAA and 1mgl-1 BA formed calli and then shoot. Apical meristem of seedling in medium supplemented with 1mgl-1 BA formed shoots which produced roots upon transferring to a medium supplemented with 2 mgl-1 IBA. Thus, this medium was the best intance for propagation and regeneration than the other media.

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References

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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 15, Issue 4 - Serial Number 4
February 2008
Pages 336-348

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