In vitro propagation of Ulmus carpinifolia through bud culture

Document Type : Research Paper

Authors

1 Research Institute of Forests and Rangelands, P.O. Box: 13185-116, Tehran, Iran

2 Research Institute of Forests and Rangelands, P.O. Box: 13185-116, Tehran, Iran.

Abstract

Abstract
Ulmus carpinifolia is an important forest tree species for timber and wood production. For investigation of asexual regeneration of Ulmus carpinifolia, apical buds from old adult trees (from kojor- Mazandaran province) were gathered in 3 seasons. After surface sterilization treatments, the best method for sterilizing of buds was identified as pretreatment, cleaning and brushing of buds with tween and 70% ethanol  solution, dipping in ethanol 70 % for 1 minutes and sterilizing buds with HgCl2  0.3% solution in different times (5 for spring, 7 for fall and 12 minutes for winter). The suitable medium for establishment was DKW with 1mg.l-1 BA. Established explants is subcultured in 2 media (MS, DKW) supplemented with 2ip, BA (0 to 1 mg.l-1) + IBA (0.01 mg.l-1). Following 3 subcultures of buds in these media, the means of shoot proliferation and shoot length growth were used as the data for analysis of variances. The best medium for shoot regeneration was DKW with 1 mg.l-1 BA, 0.01 mg.l-1 IBA (100%). Rooting of shoots occured in DKW medium supplemented with 0.5 mg.l-1 of IBA (%90). These plantlets were transferred successfully in field.
 

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References

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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 15, Issue 4 - Serial Number 4
February 2008
Pages 296-304

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