Study of similarities between bacteria isolated from Medicago sativa and M. rigidula

Document Type : Research Paper

Authors

1 Research Institute of Forests and Rangelands, P.O. Box: 13185-116, Tehran, Iran

2 Azad university, Gom

3 Research Institute of Forests and Rangelands, P.O. Box: 13185-116, Tehran, Iran.

Abstract

Medicago rigidula L. plants were collected from 3 different sites with 1450-2000m above sea level. Symbiotic Sinorhizobium bacteria were isolated from the collected plants. In addition, the symbiotic bacteria were isolated from alfalfa (Medicago sativa) as a forage crop. The isolated bacteria from M. rigidula and M. sativa L. species were identified via various biochemical characteristics, utilization of different sugars and litmus milk, tests. Responses of the bacteria to some antibiotics and different NaCl concentrations were evaluated. The in vivo experiments, including inoculation of M. rigidula seedlings with bacteria isolated from M. sativa plants, inoculation of M. sativa with bacteria isolated from M. rigidula (cross infection) as well as inoculation of M. rigidula seedlings with bacteria isolated from this species, were carried out. The results showed similarity between bacteria isolated from the annul medic and alfalfa. Further more, bacteria extracted from one species, can easily establish in root of the other species, and produce nodule. Based on the results obtained from various tests and experiments, the above mentioned bacteria isolated from the both species are sinorhizobium melliloti. However, number and size of the nodule produced by bacterium of one species differed from the Sinorhizobium melliloti isolated from the other species.

Keywords

References

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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 15, Issue 4 - Serial Number 4
February 2008
Pages 295-284

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