Study of tissue culture in dioecious sorrel

Document Type : Research Paper

Authors

1 Agricultural Biotechnology Research Institute, Iran.

2 Agricultural Biotechnology Research Institute

3 College of Agriculture, Shahed University.

Abstract

Sorrel(Rumex acetosa L.) is a perennial species, being used as a model plant in molecular genetic studies of sex determination, because of several characteristics such as dioecy, sex chromosomes and short life cycle it is In this study regeneration potentials of different tissues such as cotyledon, hypocotyl, leaf, petiole and flowering stem cultured on media containing different cytokinin and auxins combinations are presented. The results showed callus induction in cultured tissues on media containing auxins. Tissues cultured on media containing cytokinins alone or in combinations with auxins showed regeneration response. The highest regeneration rate 100% was obtained from stem segments cultured on medium containing 1 mg/l TDZ. Leaf and petiole after stem had regeneration rate of 35% and 40 % on medium containing 1 mg/l TDZ and 0.75 mg/l IAA and NAA. The highest number of regenerants per explant was 8.8 using stem segments cultured on medium containing TDZ. Histological observations showed that regenerated shoots originated from the cells in the area of the vascular tissues. Regenerated shoots rooted on medium without hormone and plants produced seeds after growth in glasshouse conditions.

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References

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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 15, Issue 4 - Serial Number 4
February 2008
Pages 273-283

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