Evaluating the effects of Funneliformis mosseae on expression of dehydrin during drought stress on Iranian pistachio (Pistacia vera L.)

Document Type : Research Paper

Authors

1 Ph.D. student, College of Forest Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I.R.Iran.

2 Associate Professor Department of Silviculture and Forest Ecology Faculty of Forest Sciences Gorgan University of Agricultural Sciences and Natural Resources (GUASNR)

3 Assoc. Prof., College of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I. R. Iran

4 Assoc. Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran

Abstract

Arbuscular mycorrhizal fungi are one of the most important and the most abundant micro-organisms in soil. The fungi have symbiotic relation with 80 to 90 percent of plant species. Arbuscular mycorrhizal symbiosis is able to alter pattern of dehydrin expression under drought stress. During period of drought acclimation that causes dehydration of cells, dehydrin proteins accumulate inside cells and protect membranes and macromolecules structures. All seedlings were irrigated three times per week during two years then were exposed to severe drought stress for nine days until wilt symptoms appeared on their leaves, then irrigated normally. It was evaluated whether Funneliformis mosseae is able to alter expression functions of dehydrin in the mycorrhiza seedlings of Pistacia vera under drought stress. Results showed that mycorrhizal colonization percentage in roots of P. vera declined significantly due to water stress treatments. The result revealed that the expression of dehydrin was increased significantly in non-mycorrhizal seedlings at 9 days exposure to the drought stress compared to the mycorrhizal seedlings, but the levels of dehydrin accumulation in mycorrhizal treatment subjected to drought were considerably lower than that of corresponding  non-mycorrhiza seedlings, indicating that the accumulation of dehydrin  proteins is not a mechanism by which the mycorrhizal symbiosis protects their host seedlings and suggesting that mycorrhizal seedlings were less strained by drought due to primary drought-avoidance mechanisms

Keywords

Main Subjects

References

-      Abbaspour, H., Saeidi-Sar, S., Afshari, H. and Abdel-Wahhab, M.A., 2012. Tolerance of Mycorrhiza infected Pistachio (Pistacia vera L.) seedling to drought stress under glasshouse conditions. Journal of Plant Physiology, 169: 704-709.
-      Aroca, R., Ferrante, A., Vernieri, P. and Chrispeels, M.J., 2006. Drought, abscisic acid, and transpiration rate effects on the regulation of PIP aquaporin gene expression and abundance in Phaseolus vulgaris plants. Annals of Botany, 98: 1301-1310.
-      Azevedo-Neto, A.D., Prisco, J.T., Enás-Filho, J., Braga, D.E., Abreu, C.E. and Gomes-Filho, E., 2006. Effect of salt stress on antioxidative enzymes and lipid peroxidation maize genotypes. Environmental and Experimental Botany, 56: 87-94.
-      Bahramabadi, E.Z., Rezanejad, F. and Hosseinali, S., 2012. Effects of cold and short day treatments on dehydrin gene expression in seedlings and regenerated shoots of pistachio (Pistacia vera L.). Journal of Plant Biology, 4(14): 35-48.
-      Barea, J.M., Azcón, R. and Azcón-Aguilar, C., 2011. Mycorrhizosphere interactions to improve plant fitness and soil quality. Antonie Van Leeuwenhoek, 81: 343-351.
-      Battaglia, M., Olvera-Carrillo, Y., Garciarrubio, A., Campos, F. and Covarrubias, A., 2008. The enigmatic LEA proteins and other hydrophilins. Journal of Plant Physiology, 148: 6-24.
-      Biermann, B. and Linderman, R.G., 1981. Quantifying vesicular-arbuscular mycorrhizae: Proposed method towards standardization. New Phytologist, 87: 63-67.
-      Birhane, E., Kuyper, T.W., Sterck, F.J., Gebrehiwot, K. and Bongers, F., 2015. Arbuscular mycorrhiza and water and nutrient supply differently impact seedling performance of dry woodland species with different acquisition strategies. Plant Ecology and Diversity, 8(3): 387-399.
-      Bray, E.A., 1997. Plant responses to water deficit. Trends in Plant Sciences, 2: 48-54.
-      Cellier, F., Conejero, G., Breitler, J.C. and Casse, F., 1998. Molecular and physiological responses to water deficit in drought-tolerant and drought-sensitive lines of sunflower. Plant Physiology, 116: 319-328.
-      Chang-Cai, L., 2012. Genome-wide identification and charactereization of a dehydrin gene family in poplar (Populis trichocarpa). Plant Molecular Biology Reporter, 30(4): 848-859.
-      Chomczynski, P. and Sacchi, N., 1987. Single step method of RNA isolation by acid guanidinum thiocyanat phenol- chloroform extraction. Analytical Biochemistry, 162: 156-159.
-      Close, T.J., 1997. Dehydrins: A commonalty in the response of plants to dehydration and low temperature. Physiologia Plantarum, 100: 291-296.
-      Colmenero-Flores, J.M., Campos, F., Garciarrubio, A. and Covarrubias, A.A., 1997. Characterization of Phaseolus vulgaris cDNA clones responsive to water deficit: identification of a novel late embryogenesis abundant-like protein. Plant Molecular Biology, 35: 393-405.
-      Forrest, K.L. and Bhave, M., 2007. Major intrinsic proteins (MIPs) in plants: A complex gene family with major impacts on plant phenotype. Functional & Integrative Genomics, 7: 263-289.
-      Gong, M.G., Tang, M. and Chen, H., 2013. Effects of two Glomus species on the growth and physiological performance of Sophor davidii seedlings under water stress, New Forest, 44: 399-408.
-      Gupta, M.L., Prasad, A., Ram, M. and Kumar, S., 2002. Effect of the vesicular arbuscular mycorrhizal (VAM) fungus Glomus fasciculatumon the essential oil yield related characters and nutrient acquisition in the crops of different cultivars of menthol mint (Mentha arvensis) under field conditions. Bioresource Technology, 81: 77-79.
-      Gutjahr, C., Banba, M., Croset, V., Miyao, A., Hirochika, H., Imaizumi-Anraku, H. and Paszkowski, U., 2008. Arbuscularmycorrhiza-specific signaling in rice transcends the common symbiosis signaling pathway. Plant Cell, 20: 2989-3005.
-      Hardie, K., 1985. The effect of removal of extraradical hyphae on water uptake by vesicular–arbuscular mycorrhizal plants. New Phytologist, 101: 677-684.
-      Karimi doost, A., 2001. Quantitative and qualitative study of natural stands of pistachio in the region Maravetappe, The East of Golestan. Final report of research project, 75p (In Persian).
-      Lara-Pérez, L.A., Noa-Carrazana, J.C., Hernández-González, S., Alarcón-Gutiérrez, E., Sánchez-Velásquez, L.R., Zulueta-Rodríguez, R. and Andrade-Torres, A., 2014. Diversity and colonization of arbuscular mycorrhizal fungi in the tree fern Alsophila firma in rainy and dry season. Symbiosis, 62(3): 143-150.
-      Liu, T., Sheng, M., Wang, C.Y., Chen, H., Li, Z. and Tang, M., 2015. Impact of arbuscular mycorrhizal fungi on the growth, water status and photosynthesis of hybrid poplar under drought stress and recovery. Photosynthetica, 53(2): 250-258.
-      Marulanda, A., Porcel, R., Barea, J.M. and Azcón, R., 2007. Drought tolerance and antioxidant activities in lavender plants colonized by native drought-tolerant or drought-sensitive Glomus species. Microbial Ecology, 54(3): 543-552.
-      Maurel, C., Verdoucq, L., Luu, D.T. and Santoni, V., 2008. Plant aquaporins: Membrane channels with multiple integrated functions. Annual Review of Plant Biology, 59: 595-624.
-      Muhsin, T.M. and Zwiazek, J.J., 2002. Colonization with Hebeloma crustuliniforme increases water conductance and limits shoot sodium uptake in white spruce (Picea glauca) seedlings. Plant and Soil, 238: 217-225.
-      Navarro, G.A., Del, P., Banón, Á.S., Morte, A. and Sánchez-Blanco, M.J., 2011. Effects of nursery preconditioning through mycorrhizal inoculation and drought in Arbutus unedo L. plants. Mycorrhiza, 21: 53-64.
-      Pfaffl, M.W., 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research, 29(9): 2001-2007.
-      Porcel, R., Azcon, R. and Ruiz-Lozano, M., 2005. Evaluation of the role of genes encoding for dehydrin proteins (LEA D-11) during drought stress in arbuscular mycorrhizal Glycine max and Lactuca sativa plants. Journal of Experimental Botany, 56(417): 1933-1942.
-      Puhakainen, T., Li, C., Boije-Malm, M., Kangasjarvi, J., Heino, P. and Palva, T., 2004. Short-day potentiation of low temperature-induced gene expression of a C-repeat-binding factor-controlled gene during cold acclimation in silver birch. Journal of Plant Physiology, 136: 4299-4307.
-      Rorat, T., 2006. Plant dehydrins—tissue location, structure and function. Cell and Molecular Biology Letters, 11(4): 536-556.
-      Ruiz-Lozano, J.M., 2003. Arbuscular mycorrhizal symbiosis and alleviation of osmotic stress: New perspectives for molecular studies. Mycorrhiza, 13: 309-317.
-      Ruiz-Lozano, J.M. and Azcón, R., 1995. Hyphal contribution to water uptake in mycorrhizal plants as affected by the fungal species and water status. Physiologia Plantarum, 95: 472–478.
-      Saavedra, L., Svensson, J., Carballo, V., Izmendi, D., Welin, B. and Vidal, S., 2006. A dehydrin gene in Physcomitrella patens is required for salt and osmotic stress tolerance. The Plant Journal, 45(2): 237-249.
-      Silva, E.M., Maia, L.C., Menezes, K.M.S., Braga, M.B., Melo, N.F. and Yano-Melo, A.M., 2015. Water availability and formation of propagules of arbuscular mycorrhizal fungi associated with sorghum. Applied Soil Ecology, 94: 15-20.
-      Urgiles, N., Straub, A., Loján, P. and Schüßler, A., 2014. Cultured arbuscular mycorrhizal fungi and native soil inocula improve seedling development of two pioneer trees in the Andean region. New Forests, 45(6): 859-874.
-      Velasco-Conde, T., Yakovlev, I., Majada, J., Aranda, I. and Johnsen, O., 2012. Dehydrins in maritime pine (Pinus pinaster) and their expression related to drought stress response. Tree Genetics and Genomes, 8(5): 957-973.
-      Yakubov, B., Barazani, O., Shachack, A., Rowland, L.J., Shoseyov, O. and Golan-Goldhirsh, A., 2005. Cloning and expression of a dehydrin-like protein from Pistacia vera L. Trees, 19: 224-230.
-      Zhang, Y., Zhong, C.L., Chen, Y., Chen, Z., Jiang, Q.B. and Wu, C., 2010. Improving drought tolerance of Causarina equisetifilia seedlings by arbuscular mycorrhizal under glasshouse conditions. New Forests, 40: 261-271.
-      Zhu, J.K., Hasegawa, P.M. and Bressan, R., 1997. Molecular aspects of osmotic stress in plants. Critical Review of Plant Science, 16: 253-277.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 25, Issue 2 - Serial Number 50
September 2017
Pages 314-323

Files

Share

How to cite

Statistics