In vitro selection of Plantago ovata for NaCl tolerance

Document Type : Research Paper

Authors

1 Faculty member of Khorasan Razavi Agricultural and Natural Resources Research Center or Mashhad Branch, Education and Extension Organization (AREEO)

2 M.Sc., Mashhad Branch, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, I.R. Iran,

3 M.Sc., Mashhad Branch, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, I.R. Iran

Abstract

Plantago ovata is from "Plantaginaceae" family which has several species. It is an annual herbaceous plant. Its height reaches to 10-40 cm. Its primary origin is India and Pakistan and it has wide distribution in Iranian flora. In order to evaluate responses of Plantago ovata to salinity an experiment was conducted using in vitro selection and tissue culture technique as well as somaclonal variation for identification and production of tolerant genotypes. The experiment was performed based on a completely randomized design with 12 treatments and 15 replications in each treatment. Hypocotyl and root explants showed the highest and the lowest callus induction respectively in non-saline (control) condition. Regeneration percentage of hypocotyl calli at all NaCl concentrations was more than cotyledon calli. Increasing concentrations of NaCl led to reduce percentage of regeneration. Cotyledon and root explants showed the highest and the lowest dry and fresh weight at all concentrations of NaCl. Also increasing of NaCl concentrations led to increment of sodium and decrement of potassium ingredients of callus. Amount of calcium varied by increasing NaCl concentration. Rooting happened a week after transferring regenerated plants to rooting medium. Then suitable seedlings were transferred to soil.

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Main Subjects

References

-      Abebe, T., Guenzi, A.C., Martin, B. and Cushman, J.C. 2003. Tolerance of mannitol-accumulating transgenic wheat to water stress and salinity, Plant Physiology, 131 (4): 1748 – 55.
-      Akhundi, M., Safarnejad, A. and Lahouti, M., 2007. Effect of drought stress on proline accumulation and elements changes in alfalfa Yazdi, Nikshahri and Renjer (Medicago sativa L.). Journal of Science and Technology of Agriculture and Natural Resources, 10 (1): 165-175.
-      Almansouri, M., Kinet, J.M. and Lutts, S. 2001. Effect of salt and osmotic stresses on germination in durum wheat. Plant and Soil, 231: 243-254.
-      Ashraf, M., Mukhtar, N., Rehman S. and Rha, E.S., 2004. Salt induced changes in photosynthetic activity and growth in a potential medicinal plant Bishop`s weed (Ammi majus L.). Photosynthetica, 42(4): 543-550.
-      Bayuelo-Jimenez, J.S. and Debouch, D.G., 2003. Growth, gas exchange, water relation and ion composition of Phaseolus species grown under saline condition. Field Crops Researche, 80: 207- 222.
-      Bohnert, H. J. and Jensen, R.G., 1996. Metabolic engineering for increased salt tolerance the next step. Australian Plant Physiology, 59:661-667.
-      Ebrahimie, E., Habashi, A.A., Ghareyazie, B., Ghannadha, M. and Mohammadi, M., 2003. A rapid and efficient method for regeneration of plantlets from embryo explants of Cumin. Plant Cell, Tissue and Organ Culture, 75:19-25.
-      Gorham, J., Wyn Jones, R.G. and McDonnel, E., 1985. Some mechanisms of salt tolerance in crop plants. Plant and Soil, 89: 15 – 40.
-      Grieve, C.M., Leseh, S.M., Francois, L.E. and Mass, E.V., 1992. Analysis of main spike yield components in salt stressed wheat.Crop Science, 32: 697 – 703.
-      Jain, R.K., Jain, S., Nainawatee, H.S. and Chowdhury, J.B., 1990. Salt tolerance in Brassica Juncea L. In vitro Selection, Agronomic Evaluation and Genetic Stability, 48: 141-152.
-      Kerepesi, H. and Galiba, G., 2000. Osmotic and salt stress Induced alteration in soluble carbohydrate content in wheat seedling. Crop Science. 40: 482-487.
-      Khorrami, A. Safarnejad, A. and Shourvarz, M., 2011. Effect of salt stress on ion distribution and proline accumulation in Foeniculum vulgare using in vitro technique. International Journal of Science and Nature, 2(2): 168-175.
-      Khorrami, A. and Safarnejad, A., 2011. In vitro selection of Foeniculum vulgare for salt tolerance. Notulae Scientia Biologicae, 3(2): 90-97.
-      Konigshofer, H., 2005. Changes in ion composition and hexitol content of different Plantago species under the influence of salt stress. Plant and Soil, 72 (2-3): 289 – 296.
-      Mano, Y. and Takeda, K., 1997. Mapping quantitative trait loci for salt tolerance at germination and the seeding stage in barley (Hordeum vulgare L.). Euphytica, 94: 263 – 272.
-      Megdiche, W., Ben Amor, N., Debez, A., Hessini, K., Ksouri, R., Zuily-Fodil, Y. and Abdelly, C., 2007 . Salt tolerance of the annual halophyte Cakile maritima as affected by provenance and developmental stage. Acta Physiologiae Plantarum, 29 (4): 375 – 384.
-      Meloni, D.A., Oliva, M.A., Ruize, H.A. and Martinez, C.A., 2001. Contribution of proline and inorganic solutes to osmotic in cotton under salt stress. Plant Nutrition, 24(3) : 599-612. 
-      Miller, C. O. and Skoog, F., 1953. Chemical control of bud formation in Tabacco stem segments. American Journal of Botany, 40: 768 -773.
-      Mills, D., Zhang, G. and Benzioni, A., 2001 . Effect of different salts and of ABA on growth and mineral uptke in Jojoba shoots grown in vitro , Journal of Plant Physiology, 158 : 1031 – 1039.
-      Munns, R. and Schachtman, D.P., 1993. Plant responses to salinity significance in relation to time. International Crop Science, 1: 741-745. 
-      Najafi, F., Khavari-Nejad, R.A. and Siah Ali, M., 2010. The effects of salt stress on physiological parameters in summer savory (Satureja hortensis L.) Journal of Stress Physiology and Biochemistry. 6(1): 14-21.
-      Pramanik, S., Sen Raychaudhuri, S. and Chakraborty, S., 1995. Changes in esterase and superoxide dismutase isozymes during in vitro morphogenesis in Plantago ovata Forssk. Plant Cell, Tissue and Organ Culture, 44 (2) : 123–127.
-      Safarnejad, A., Collin, H., Bruce, K.D. and McNeilly, T., 1996. Characterization of alfalfa following in vitro selection for salt tolerance. Euphytica, 92: 55-61.
-      Safarnejad, A., Salami, M.R. and Hamidi, H., 2008. Study of morphologic characteristics medicinal plants (Plantago psyllium and  Plantago ovata) against salinity stress. Pajuhesh and Sazndegi, 75: 152-160.
-      Safarnejad, A., Shoorvarzi, M. & M. Dalir, 2016. In vitro selection of Plantago psyllium L. for salt tolerance and Changes of Sodium, Calcium and Potassium levels at callus stage, Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 24(2): 221-231.
-      Shanjun, T.U., Sangwan, R.S. and Sangwan –Norrell, B.S., 2005. Improved efficiency of somatic embryogenesis from zygotic embryos in Hyoscyamus niger by seed water – soaking, Scientia Horticulturae, 106 (3): 440 – 445.
-      Shannon, M.C., 1986. Breeding, selection and the genetics of salt tolerance .In Salinity Tolerance in Plants. (Eds: Staples, R.C. and Toenniessn, G.H.) John Wiley and Sons, 231 – 252.
-      Singh, L. and Pal, B., 2001. Effect for saline water and fertility levels on yield, Potassium, Zinc content and uptake by blonde psyllium (Plantago ovata Forsk.). Crop Research (Hisar), 22: 424 – 431.
-      Smekens, M.J. and Van Tienderen, P., 2001. Genetic variation and plasticity of Plantago coronopus under saline conditions .Acta Oecologica, 22: 187 – 200.
-      Tal, M. and Gavish, V., 1973. Salt tolerance in the wild relatives of the cultivated tomato. Australian Journal of Agricultural Research, 24: 353 – 367.
-      Vicente, O., Boscaiu, M., Naranjo, M.A., Estrelles, E., Jose, J.M., Belles, M. and Soriana, P., 2004. Responses to salt stress in the halophyte Plantago crassifolia (Plantaginaceae). Journal of Arid Environment, 58: 463 – 481.
-      Wakhlu, A.K. and Barna, K.S., 1989. Callus initiation, growth and plant regeneration in Plantago ovata Forssk. Cv.GI-2. Plant Cell, Tissue and Organ Culture, 17: 235 – 241.
-      Yokoi, S., Bressan, A. and Hasegawa, P.M., 2002. Salt stress tolerance of plants. JIRCAS Working Report, 25-33.
-      Zargari, A., 1997. Medicinal Plants. Sixth Edition. Print and Publications Institute of Tehran University. Volume 4. 852 p.
 
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 25, Issue 2 - Serial Number 50
September 2017
Pages 289-301

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