Genetic diversity among fennel (Fueniculum vulgare Mill.) accessions using morphological and SCoT markers

Document Type : Research Paper

Authors

1 *- Associ. Prof., Department of Agriculture, Payame Noor University, I.R. Iran

2 M.Sc. in biotechnology, Razi University. Kermanshah, I.R.Iran

3 Prof., Department of Agronomi and Plant breeding. Razi University. Kermanshah, I.R.Iran

4 Lecturer, Department of Agriculture, Payame Noor University, I.R. Iran

Abstract

    Fennel (Foeniculum vulgare Mill.) is one of the well-known medicinal and aromatic plants. To determine variability between 16 Iranian fennel accessions based on a molecular marker and morphological traits an experiment was carried out based on a completely randomized design with three replications at Payame Noor University of Kermanshah, Iran, during 2014. Eleven morphological traits were recorded. Genotypes were evaluated based on 10 SCoT primer markers as well. Analysis of variance showed significant differences among the genotypes for the studied characters. Mean comparison according to DMRT was carried out and significant differences were revealed for all of the studied traits (p≤0.05). Cluster analyses classified the accessions into four groups. Principle components analysis revealed that 81.4 percent of variance explained by four PCAs. The strongest heritability (%71.37) belonged to main stem. SCot primers showed 55 visible bands. SC5 and SC29 primers had the most number of bands with 10 and 9 bands respectively. Means of polymorphic information content (PIC=0.36), marker index (MI= 1.82), effective multiplex ratio (EMR= 4.8) and resolving power (RP= 5.86) indices were calculated for all primers. Total genetic similarity based on the primers was 0.66 percent. Cluster analysis classified the accessions into 3 groups.The clustering results was confirmed by principal coordinate analysis.

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Main Subjects

References

-      Altıntas, S., Toklu, F., Kafkas, S., Kilian, B., Brandolini, A. and Zkan H O., 2008. Estimating genetic diversity in durum and bread wheat cultivars from Turkey Using AFLP and SAMPL markers. Plant Breeding, 127: 9-14.
-      Bahmani, K., 2001. Study of morphological and genetic diversity of fennel populations. M.Sc. Thesis, Tehran University. Iran. (In Persian)
-      Bahmani, K., Izadi darbandi, A. and Baghchghi, R., 2012. The study of genetic variation Iranian fennel using RAPD molecular marker. Fourteenth Iranian Genetics Congress (In Persian).
-      Chawla, H S., 2000. Introduction to Plant Biotechnology. Science Publishers Inc. USA
-      Hamza, S., Hamida, W.B., Rebai, A. and Hrrabi, M., 2004. SSR-based genetic diversity assessment among Tunisian winter barley and relationship with morphological traits. Euphytica, 135:107-118.
-      Hasani, M.H., Torabi S., Omidi, M., Etminan, A.R. and Dastmalchi T., 2011. The study of genetic variation Foeniculum vulgare Mill. Using AFLP molecular marker. Iranian Journal of Field Crop Science, 42(3):597-604. (In Persian).
-      Hou, Y., Yan, Z. and Wei, Y., 2005. Genetic diversity in barely from west China based on RAPD and ISSR analysis Barely. Genetics Newsletter, 35:9-22.
-      Izadi Darbandi, A. and Bahmani, K., 2011. Principle of Fennel Cultivation and Breeding. Tamadone Pars Publishing. (In Persian).
-      Kalendar, R., 2007. Fast PCR: a PCR primer design and repeat sequence searching software with additional tools for the manipulation and analysis of DNA and protein. Available at www.biocenter. helsinki.fi/programs/fastpcr.htm.
-      Kumar, M., Mishra, G. P., Singh, R., Kumar, J., Naik, P. K. and Singh, Sh.B., 2009. Correspondence of ISSR and RAPD markers for comparative analysis of genetic diversity among different apricot genotypes from cold arid deserts of Trans-Himalayas. Physiology and Molecular Biology of Plants, 15(3): 225-236.
-      Maghsoudi Kelardashti, H., Rahim Malek, M., Sabz alian, MH. and Talebi, M., 2014. Study of genetic variability using morphological traits of fennel populations. Taxonomy and Biosystematics, 6(18): 77-86. (In Persian)
-      Mantel, N., 1967. The detection of disease clustering and a generalized regression approach. Cancer Research, 27: 209-220.
-      Mohamadi, S.A., 2006. Analysis of molecular data in genetic variability research. 9th Congress of Agronomy and Plant Breeding. 96-119.
-      Mohammadi, S.A. and Prasanna, B.M., 2003 .Analysis of genetic diversity in crop plants –salient statistical tools and considerations. Crop Science, 43:1235-1248.
-      Naghavi, M., Gharayazi, B. and Hosseini, GH., 2007. Molecular markers. Tehran University Publishing. (In Persian)
-      Omidi, M. and Farzin, N., 2009. Strategical biotechnology increasing efficiency of medicinal plant. Conference of Food and Biotechnology. Islamic Azad University of Kermanshah. (In Persian).
-      Powell, W., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S. and Rafalski, A., 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding, 2: 225-238.
-      Rostaei, M.K., Hassani, M., Hossainpor, T. and Kalate, M., 2014. Stability of grain yield of wheat genotypes in tropical and subtropical region. Iranian Journal of Agricultural Sciences, 35: (2) (In Persian).
-      Safaei, L., Zainali, H. and Afioni, D., 2011. Study of genetic variability of fennel genotypes using agronomic traits. Iranian Journal of Rangelands and Forest plant Breeding and Genetic Research. 19(1): 167-180. (In Persian).
-      Safaei, L., Zainali, H. and Afioni, D., 2014. Grain yield comparison and yield components in fennel genotypes. Journal of Seed and Seedlings Breeding, 2(1-3): 289-303 (In Persian).
-      Schaut, J.W., Qi, X. and Stam, P., 1997. Association between relationship measures based on AFLP markers, pedigree data and morphological traits in barley. Theoretical and Applied Genetics, 95: 1161-1168.
-      Shabanian, N., Alikhani, L. and Rahmani, M.S., 2015. Phenotypic and genotypic diversity in rant oak (Quercus brantii) populations of declining North-Zagros forests using biochemical characteristics and molecular SCoT marker. Iranian Journal of Rangelands and Forests Plant Breeding and Genetics Research, 23(1): 23-29. (In Persian)
-      Sharma, M. and Meena, RS., 2013. Genetic Diversity in Fennel (Foeniculum vulgare). International Journal of Scientific Reaserch, 2(8): 2277-8179.
-      Torres, A.M., Weeden, N.F. and Martin, A., 1993. Linkage among isozyme, RFLP and RAPD markers in Vicia faba. Theoretical and Applied Genetics, 85: 935–945.
-      Zahid, N.Y., Abbasi, N.A., Hafiz, I.A. and Ahmad, Z., 2009. Genetic diversity of indigenous fennel, (Foeniculum vulgare. germplasm in Pakistan assessed by RAPD markers. Pakistan Journal of Botany, 41(4): 1759-1767.
-      Zhahid, N., Abbasi, A.N., Hafiz, A. and Ahmad, Z., 2008. Morphological characteristics and oil contents of fennel (Foeniculum vulgare) accessions from different regions of Pakistan. Journal Chem. Soc. Pak., 30:6.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 25, Issue 2 - Serial Number 50
September 2017
Pages 212-231

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