Studying the effects of media and micro-explants on optimization of Alhagi camelorum F. culture

Authors

1 Corresponding author, M.Sc., Range Management, Gorgan University of Agricultural Sciences and Natural Resources, I.R.Iran

2 Prof., Dept. of Range Management, Gorgan University of Agricultural Sciences and Natural Resources, I.R. Iran

3 Assoc. Prof., Research Center of Agricultural and Natural Resources of Golestan Province, Agricultural Research, Education and Extension Organization (AREEO), I.R. Iran.

Abstract

Camel thorn (Alhagi camelorum F.), a plant from Fabaceae family, has a great adaptation in arid conditions. Due to high digestible proteins, possibility of extracting manna from its stems for medicinal purposes and its high tolerance to harsh arid environments, it is considered as an important forage and medicinal plant. It grows via seed and rhizome, but its seed germination rate is very low. Aim of the research was to investigation possible effects of different sucrose concentration levels in micro- propagation of the species via ovary and seed embryo culture to improve its propagation. Seed germination of the species was tested by two explant treatments of ovary and seed embryo in 5 sucrose levels of 0, 15, 30, 45 and 60 gr/l concentrations in MS medium in 5 replications. Germination rate was recorded during four weeks. Results of comparison of germination rates between the two explant treatments in different sucrose concentrations showed that the percentage of embryo germination and seedling height was significantly higher than that of ovary culture. The highest significant embryo germination rate (93/3%) was recorded on 15 gr/l sucrose concentration. By increment of sucrose concentration to 15 gr/l, embryo attributes such as radicle length, plumule length, seedling length, and seed germination index also increased. By increasing sucrose concentration over 15 gr/l, reverse effect was observed on all of the studied attributes.

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References

Ali, S.I., 1977. Flora of West Pakistan, pp 319–320.
-Arnold, S.V., 1987. Improved efficiency of somatic embryogenesis in mature embryos of Picea abies (L.) Karst. Journal of Plant Physiology, 128: 233-244.
-Bazoobandi, M., Barati, M. and Haghighi, S., 2006. Physiological response of Alhagipseudoalhagi to root exhausting management during fallow season. Iranian Journal of Weed Science, 2: 84-95.
-Burrell, A.M., Lineberger, R.D., Rathore, K.S. and Byrne, D.H., 2006. Genetic variation in somatic embryogenesis of Rose. Hort Science, 41: 1165-1168.
-Calagari, M., Jafari Mofidabadi. A., Tabari, M. and Hosseini, S.M., 2004. Intraspecific hybridizationin Populus euphratica Oliv. Using in vitro embryo rescue technique. Pajouhesh & Sazandegi, 61: 6-9.
-Emam, M., Ghamari Zare, A., Asadicorom, F. and Looki­ Anaraki. K., 2013. Micropropagation of Amygdalus scoparia L. by bud and embryo culture. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 21: 77-86.
-Gram, T., Mattsson, O. and Joersbo, M., 1996. Division frequency of pea protoplasts in relation to starch accumulation. Plant Cell, Tissue and Organ Culture, 45: 179-183.
-Guerra, M.P., Silveira, V., Dos Santos, A.L.W., Astarita, L.V. and Nodari, R.O., 2000. Somatic embryogenesis in Araucaria angustifolia (BERT) O .Ktze. In Jan, M.S., Gupta, P.K. and Newton, R.J. (ed.), Somatic Embryogenesis in Woody Plants. Kluwer Academic Publishers. Netherlands, 6: 457-478.
-Ivanica, J. and Mokra, A., 1982. Development and cultivation of early-ripening cherry embryos. Biologia Czechoslovakia, 37: 1, 3-12.
-Jafari Mofidabadi, A., 2015. Production of inter-specific hybrid between Populus caspica and P.nigra using mature embryo culture. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 23:49-55.
-Jafari Mofidabadi, A. and Modir-Rahmati, A.R., 2000. Production of Populus euphratica Oliv. × P. alba L. hybrid poplars through ovary and ovule cultures. Plant Genetic Newsletter, 122:13-15.
-Karami, O., Deljou, A. and Bahmani, R., 2008. Type and concentration role of sugar in somatic embryogenesis of carnation (Dianthus caryophyllus L.) cultivar of Nelson. Journal of Horticulture Science, 22: 13-21.
-Khan, F.M., 2009. Ethno-veterinary medicinal usage of flora of greater Cholistan desert (Pakistan). Pak Vet J, 29: 75–80.
-Kim, C.K., Oh, J.Y., Chung, J.D., Burrel, A.M. and Byrne, D.H., 2004. Somatic embryogenesis and plant regeneration from in-vitro-grown leaf explant of rose. Hort Science, 39: 1378-1380.
-Kumar, J. and Gupta, P.K., 2008. Molecular approaches for improvement of medicinal and aromatic plants. Plant Biotechnology Reports, 2: 93-112.
-Liyun, H., Jie, L., Huanqi, Z. and Haikuo, F., 2012. Study on the mix recipe screening in Areca catechu embryo culture. Chinese Agricultural Science Bulletin, 34, 014.
-Litz, R.E., 1986. Effect of osmotic stress on somatic embryogenesis in Caricasuspension cultures. J. Amer. Soc. Hort. Sci., 111: 969-970.
-Liu, G.S., Liu, J.S., Qi, D.M., Chu, C.C. and Li, H.J., 2004. Factors affecting plant regeneration from tissue cultures of Chinese leymus (Leymus chinensis). Plant Cell, Tissue and Organ Culture, 76: 175-178.‏
-Majd, A., Chamandosti, F., Mehrabia, S. and Sheidai, M., 2006. Somatic embryogenesis and plant regeneration in Brassica napus L. Biological Science, 9: 729-734.
-Marwat, S.K., Khan, M.A., Ahmad, M., Zafar, M. and Rehman, F., 2008. Ethnophytomedicines for treatment of various diseases in D. I. Khan district. Sarhad J. Agric., 24: 306–316.
-Merkle, S.A., Parrott, W.A. and Flinn, B.S., 1995. Morphogenic aspects of somatic embryogenesis. In: Thorpe, T.A. (ed.): In vitro embryogenesis in plants. Kluwer Academic Publishers, Netherlands: 1-16.
-Modares, M., Lahuty, M., Ganj Ali, A. and Asili, J., 2014. Optimization of in vitro culture of embryos Zygotic S. leriifolia (Salvia leriifolia Benth.). Journal of Horticultural Science, 28: 319-326.
-Mohan, N., Nikdad, S. and Singh, G., 2011. Studies on seed germination and embryo culture of Jatropha curcas L. under in vitro conditions. Research Article, Biotechnol, Bioinf, Bioeng, 1: 187-194.‏
-Murashige, T. and Skooge, F., 1962. A revised medium for rapid growth and bio- assays with tobacco tissue culture. Physiologia Plantarum, 15: 473-597.
-Naraghi, T.S., 2014. In vitro propagation of yew (Taxus baccata) by culture of sexual embryos. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 12:335-343.
-Piri Zirkouhi, M., Mashayekhi, K., Kamkar, B., Hemmati, K.h. and Vahdatpour, F., 2009. Embryogenesis of a commercial and a native tomato cultivar using different culture media. J. of Plant Production, 16:101-114.
-Rai, M.K., Akhtar, N. and Jaiswal, V.S., 2007. Somatic embryogenesis and plant regeneration in Psidium guajava L. cv. Banarasi local. Scientia Horticulture, 113: 129-133.
-Rodriguez, R., Berros, B., Centeno, M.L., Rovira, M., Rodrigues, A. and Radojevic, L., 2000 Applied and basic studies on somatic embryogenesis in hazelnut (Corylus avellana L.), In: Jan, M.S., Gupta, P. and Newton, R.J. (eds.), Somatic Embryogenesis in Woody Plants. Kluwer Academic Publishers. Dordrecht-The Netherland, 6: 291-359.
-Samsam-Shariat, S.H., 1992. Qualitative and quantitative evaluation of the active constituents and control methods for medical plants. Isfahan: Mani Publications, pp: 23-30.‏
-Sanchez-Zamora, M. Á., Cos-Terrer, J., Frutos-Tomás, D. and García-López, R., 2006. Embryo germination and proliferation in vitro of Juglans regia L. Scientia Horticulturae, 108: 317-321.‏
-Takamura, T. and Miyajima, I., 1997. Micropropagation of Cyclamen persicum Mill. In: Bajaj, Y.P.S. (ed) Biotechnology in Agriculture and Forestry, Vol. 40: High-Tech and Micropropagation. Springer Verlag, Berlin, Heidelberg: 96-112.
-Varshney, K., and Singh, A.K., 2008. Inventory of some ethnomedicinal plant species used by rural people of Etah district, UP, India. Plant Arch, 8: 757–759.

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