Effects of in vitro polyploidy induction on some morphological, physiological and biochemical traits of Salvia leriifolia Benth

Document Type : Research Paper

Authors

1 Assoc. Prof., Horticulture Department, Urmia University, Urmia, I.R. Iran

2 M.Sc., Horticulture Department, Urmia University, Urmia, I.R. Iran

Abstract

Salvia leriifolia Bent. is a perennial herbaceous plant belongs to Lamiaceae family. Different pharmacological activities of the species were evaluated during recent years. Medicinal plant In vitro ploidy induction was one of the interesting issues in biotechnology and plant tissue culture. Therefore, influence of different colchicineconcentrations (0, 0.05, 0.1, 0.2, and 0.5 %) in three time duration levels (24, 48 and 72 hours) on ploidy induction of in vitro regenerated seedlings of the species was studied. For detection of polyploid plants, morphological, physiochemical, biochemical and cytogeneticall characteristics were analyzed. ANOVA results revealed that, the effects of colchicine concentrations on explants survival rate were significant at p < 0.01. Among the colchicine treatments the highest seedling survival rate (84.44%) was observed by 0.05% treatment. Although the most dead plants, were recorded in high colchicine concentration (0.5%). Induction of tetraploidy in Salvia Leriifolia accompanied by significant changes in different morphological, cytological, physiological and physiochemical characteristics such as, increasing length and width of stomata on leaf surface, leaf width, leaf area, leaf chlorophyll content, total antioxidant levels, total phenol and antioxidant enzyme activity (catalase and ascorbate peroxidase), as well as reducing density of stomata per unit leaf area and leaf length. Chromosome number of diploid plants was 2n = 2x = 22, and in tetraploid plants was
2n = 4x = 44. Therefore, colchicine effectively induces polyploidy in the species.

Keywords

Main Subjects

References

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