Cytogenetic study of Plantago major populations in North West of Iran

Document Type : Research Paper

Authors

1 M.Sc., Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, I.R. Iran,

2 Prof., Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, I.R. Iran

3 Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, I.R. Iran

4 M.Sc., Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, I.R. Iran

Abstract

Plantago genus of Plantaginaceae family has 22 annual and perennial herbaceous species in Iran. Plantago major is one of common plants in wetlands. Chromosomal characteristics of seven populations of Plantago majorL. were studied. Seeds were collected from habitats of the species in NW of Iran. The seeds were grown in petri dishes. Root tip meristems were stained with Hematoxilin. Chromosome number of five populations was 2n= 12 and in two other populations was 2n=24. Base Chromosome number of the species was x=6. In studied populations, Karyotype formula had metacentric and submetacentric chromosomes. Chromosome size of the species varied between 1.25 to 2.87 µm. Ideogram of each population was made and karyotypic characteristics including chromosome number, long and short arm length, and arm ratio indices were also estimated. The species had symmetrical karyotype in all of the examined populations; According to Stebbins classification, six of the studied populations located in 1A class and one of the populations located in 2A class.

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Aryavand, A. 1980. In: Love, A. (ed): IOPB chromosome number reports IXIX.-Taxon 29: 704.
-Badr, A. and El-Kholy, M.A., 1987. Chromosomal studies in the Egyptian flora II. Karyotype studies in the genus Plantago L. Cytologia, 52: 725-731
-Chiang, L.C., Chiang, W., Chang  M.N.L. and Lin, C.C., 2002. Antiviral activity of Plantago major extracts and related compounds in vitro. Antiviral Research, 55:1: 53-62.
- Dhar, K., 2006. Characterization and physical mapping of ribosomal RNA gene families in Plantago. Annals Botany, 97: 541–548.
-Ebadi-Almas, D., Karimzadeh Gh. and Mirzaghaderi, Gh., 2012. Karyotypic_ variation and karyomorphology_in_Iranian_endemic_ecotypes_of_Plantago_ovata, Cytologia, 77: 215–223.
-Ghorbanli, M., Sateei, A. and Nasiri- Savadkohi, S., 2011. Effect of various concentrations of copper on antioxidant enzymes activity and phenolic compounds content in leave and roots of Plantago major L., Iranian Journal of Medical and Aromatic Plant, 26:4:50.
-Huziwara Y., 1962. Karyotype analysis in some genera of Compositae. VIII. Further studies on the chromosome of Aster. American Journal of Botany, 49: 116-119.
-Levan, A., Fredka, K. and Sandberg, A., 1964. Nomenclature for centromeric position on chromosome.
-Mohsenzadeh, S., Nazeri, V. and Mirtadzadini, S.M., 2008. Chromosome numbers of fifteen species of Plantago L. (Plantaginaceae) from Iran. Iran Journal of Botany, 14 (1)
-Mozaffarian,V., 2008. A Dictionary of Iranian Plant Names. Farhang Moaser,Tehran, Iran.
- Peruzzi, L., 2003. Mediterranean chromosome number reports 13 (1344-1347). Flora Mediterranea, 13: 370–373
-Romero-Zarco, C., 1986. A new method for estimating karyotype asymmetry, Taxon, 35:526-530.
-Shariat, A., 2013. Karyology of Iranian endemic Satureja (Lamiaceae) species. Cytologia, 78: 305–312.
- Sharifnia, F. and Albouyeh, R.M., 2002. Chromosome numbers of Plantago boissierii Hauskn. et Bornm. and Boiss. in Iran. Chromosome Science, 6: 47–48.
-Stebbins, G.L., 1971. Chromosome Evolution in Higher Plants. Edvard Amold Publisher, London.
- Wiltshire, R.J.E. and Jackson, W.D., 2003. Index of chromosome numbers of Tasmanian spermatophytes. Papers and Proceedings of the Royal Society Tasmania, 137: 39–53.