Production of pharmaceutical active ingredients via hairy root induction of Birch (Betula pendula)

Document Type : Research Paper

Authors

1 Ph.D student, Faculty of forest sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I.R.Iran

2 Assis. Prof., Faculty of Forest Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I.R.Iran

3 Assis. Prof., Department of Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, I.R.Iran

Abstract

Betula pendula Roth produces a range of biologically active terpenoids such as betulin and betulinic acid. The compounds with cytotoxic effects which have been used for their medicinal properties. Agrobacterium-infected hairy roots are characterized by high growth rate and genetic stability that can beprovided noticeable amounts of the biological material. In this study, production of hairy roots by B. pendula was investigated on different explants, media and A. rhizogenes strains. Growth rate of hairy roots were studied during 20 days and the terpenoids content (betulin & betulinic acid) were assayed by HPLC. Hairy root cultures were established 20 days after infection by infecting bark segments of B. pendula by Agrobactrium strains of C58C1 and LB9402 in WPM medium. Growth of hairy root by lines of C58C1 strain (D and E lines) and one line from LB9402 strain (LB1) were significantly better than others. The most betulin content was observed in the hairy roots, 8 days after culture, which was about 0.47 mg  g-1 dry waight (DW). Furthermore, the most amount of antioxidant activity (75%) was assessed on E line roots, 20 days after cultures. Although the highest content of betulinic acid (0.6 mg g-1 DW ) was obtained by LB1 lines but betulin content and antioxidant activity were considerably less than other hairy root lines. Results indicated that due to higher relative performance, E line of hairy roots could be used for analysis of terpenoids and pharmaceutically important metabolites in future studies.

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References

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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 24, Issue 2 - Serial Number 48
December 2016
Pages 165-176

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