Investigation of genetic variability in fennel (Foeniculum vulgare) using karyotype analysis

Document Type : Research Paper

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Abstract

     Fennel (Foeniculum vulgare Mill.) is an important, well-known aromatic and medicinal herb. In order to evaluate karyotipic variation and relationship between karyotipic traits of 16 Iranian Foeniculum vulgare accessions, an experiment was conducted based on a completely randomized design with five replications at biotechnology laboratory of Payame Noor University of Kermanshah, Iran. Using squash method several kariotipic characters were measured. All of the 16 fennel accessions had 22 chromosomes. Analysis of variance showed significant differences among the accessions for the studied traits. There was significant variation among the accessions for total chromosome form. There were significant differences between the groups for the karyotypic traits. Two satellites were observed in the accessions 14726 (Markazi), 8615 (Ardabil), 29667 (Ghazvin). One satellite was also observed in the other studied accessions. Based on Stebbins table, all of the accessions, except 18650 (Ardabil) located in 1A class while Ardabil accession located in 2A class. Hormozgan (24482) accession had minimum asymmetrical index while Ardabil accession had the greatest amount of Asymmetrical index value. Cluster analysis classified the accessions into 4 groups. Principle components analysis revealed that 99 percent of variance was explained by the first two principal components.

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References

-      Abdemishani, S. and Shahnejate Bousheri, A., 2008. Complementary Plant Breeding. Vol. 1, Tehran University Press, Iran Pp. 271, (In Persian).
-      Bernath, J., 2000. Medicinal and Aromatic Plants Mezo. Publ. Budapest. pp 667.
-      Datta, A.K. and Rita, P., 2003. Chromosomal studies in four seed species of Umbelliferae. Indian Journal of Genetic and Plant Breeding, 63: 361-362.
-      Ehdaei, B., 1994. Plant Breeding. Mashhad University Press, Iran, P. 456, (In Persian).
-      Estilai, A. and Hashemi, A., 1990. Chromosome number and meiotic behavior of cultivated chia, salvia, hisponica. Hort Sciense, 25:1646-164
-      Izadi Darbandi, A. and Bahmani, K., 2011. Principle of Fennel Cultivation and Breeding. Tamadone Pars, Iran, Pp. 271, (In Persian).
-      Maghsoudi Kelardashti, H., Rahim Malek, M., Sabzalian, M. and Talebi, M., 2013. Study of genetic variation of morphological traits and heritability of Iranian Fennel (Foeniculum vulgare Mill.). Journal of Taxonomy and Biosystematic, 18: 77-86.
-      Maude, M. and Pamela. F., 1939. The merton catalogue. A list of the chromosome numerals of species of British flowering plants. New Phytologist, 38: 1-31.
-      Michaud, R., Tremblay, G.F., Belanger, G. and Michaud, M., 1990. Proteolysis in alfalfa silages made from different cultivars. Journal Agriculture and Agri-Food Canada, Soils and Crops Research and Development Center, 2560 Hochelaga blvd, Sainte-Foy, Quebec, Canada, G1V 213.
-      Mirzaie-Nodoushan, H., Mehrpur, Sh., Rezaie, M. and Rashvand, S., 2002. Primary karyotypic investigation on several populations of Aloe litoralis. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 9: 49-84, (In Persian).
-      Omidi, M. and Farzin, N., 2009. Biotechnological strategies in increasing efficiency of medicinal plants. Regional conference of Food and Biotechnology. Islamic Azad University of Kermanshah, 55-60, (In Persian).
-      Principe, P.P., 1988. Evaluating the diversity of Medicinal Plants. The Conservation of Medicinal Plants. Proceedings of an International Consultation, 21-27 Chiang Mai, Thailand. Cambridge University Press, 79-124.
-      Safae, L., Zainali, H. and Jaber-al-ansar, Z., 2008. Karyotype study of five Fennel (Foeniculum vulgare Mill.). Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 16: 117-125, (In Persian).
-      Safae, L., Zainalim, H. and Afiuni, D., 2011. Study of genetic variability based on morphological traits between fennels (Foeniculum vulgare Mill.) genotypes. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 1: 167-180, (In Persian).
-      Seetharaman, N., Dhanavel, D. and Pavadai, P., 2004. Unique features of growth in some members of Apiaceae. Plant Archives, 4: 443-446.
-      Sharma, A.K. and Ghosh, C., 1954. Cytogenetics of some of the Indian Umbellifers.Genetica Journal. 8:17-44.
-      Sheidaie, M., Kalhor-home, N. and Poorneydanei, A., 2007.Cytogenetic study of some populations of Foeniculum vulgare (Umbelliferae) in Iran. Caryologia, 60: 257-261.
-      Shojaeifar, S., 2012. Study of morphological and molecular variation of in Iranian native Fennel (Foeniculum vulgare Mill.) genotypes. M.Sc. Thesis. Technical University of Isfahan, Iran, Pp. 195, (In Persian).
-      Stebbins, G. L., 1971. Chromosomal Evolution in Higher Plants. Edward Arnold, London.
-      Yazdani, D., Shahbazi, S. and Saifi, H., 2004. Cultivation and Harvesting of Medicinal Plants. Vol. 1, Research Institute of Medicinal Plant of Jihade Daneshgahi, Iran, pp. 245, (In Persian).
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 24, Issue 1
May 2016
Pages 142-155

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