Effects of plant growth regulators and culture media on shoot proliferation and reduction of vitrification of Prunus mahaleb L. via in vitro culture

Document Type : Research Paper

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Abstract

Effects of different concentrations of plant growth regulators and culture media on shoot proliferation and vitrification of Prunus mahaleb L. via in vitro culture was studied using single node explants in twoseparate experiments. Shoots from single node explants were grown on 26 treatments (first experiment) of MS medium supplemented with different concentrations of cytokinins (BAP, TDZ, KIN, 2iP, and Zeatin) in combination with NAA and on 16 treatments (second experiment) of MS, 1/2 MS, WPM and DKW media supplemented with different combinations of CaCl2 and NH4NO3 and cytokinins of BAP, Zeatin, and PG. Experiments were based on completely randomized design with five replicates and each replication included 5 explants. Means comparison after 2 subcultures in the first experiment showed that the highest shoot length (3.14 cm) were obtained on 0.1 mg l-1 NAA in cytokinin -free medium, the highest number of shoot (14.67 shoot numbers per explants) on 0.2 mg l-1 Zeatin plus 0.1 mg l-1 NAA, and the lowest vitrification on 0.1 mg l-1 KIN, 0.05 and 0.1 mg l-1 Zeatin plus 0.1 mg l-1 NAA, respectively. In the second experiment, the highest shoot length (2.88 cm) were observed on DKW medium with 0.2 mg l-1 Zeatin plus 40 mg l-1 PG and the highest number of shoot (19 shoot number per explants) on MS medium with 880 mg l-1 CaCl2 and 825 mg l-1 NH4NO3. The present research by accessing to the highest proliferation (19 shoot numbers per explants) and decreasing vitrification of Prunus mahaleb L. provided a possibility of doing other research projects for protocol commercialization.

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References

-      Ahmadloo, F., Tabari Koochaksaraei, M., Azadi, P., Hamidi, A. and Beiramizadeh, E., 2014. Micropropagation of Crataegus pseudoheterophylla Pojark. via in vitro culture. Journal of Wood and Forest Science and Technology, 21: 1-23. (In persian)
-      Alizadeh, M., 2012. A User Manual Plant Tissue Culture and Micropropagation. Norouzi Publication, Gorgan, 322 pp. (In persian)
-      Ansar, A., Touqeer, A., Nadeem, A.A. and Eshfaq, A.H., 2009. Effect of different media and growth regulatorson in vitro shoot proliferation of olive cultuvar 'Moraiolo'. Pakistanian Journal of Bottany, 41: 783-795.
-      Banno, K., Yoshida, K., Hayashi, S. and Tanabe, K., 1989. In vitro propagation of japanese pear cultivars. Journal of the Japanese Society for Horticultural Science, 58: 37-42.
-      Bennett, I.J., McComb, J.A., Tonkin, C.M. and Mcdavid, D.A.J., 1994. Alternating cytokinins in multiplication media stimulates in vitro shoot growth and rooting of Globulus Labill. Annals of Botany, 74: 8-53.
-      Curir, P., Sumerect, V. and Termini, A., 1990. Flavonoid accumulation is correlated with adventitious root formation in Eucalyptus gunnii Hook micropropagated through axillary bud stimulation. Plant Physiology, 92: 1148-1153.
-      Driver J.A. and Kuniyuki A.H., 1984. In vitro propagation of Paradox walnut Juglans hindsii × Juglans regia rootstock. Horticultural Science, 19: 507- 509.
-      Fisichella, M., Silvi, E. and Morini, S., 2000. Regeneration of somatic embryos and roots from quince leaves cultured on media with different macroelement composition. Plant Cell, Tissue and Organ Culture, 63: 101-107.
-      Gangi Moghadam, E., Bolandi, A.R. and Anahid, S., 2008. Micropropagation of four selected dwarf mahaleb (Prunus mahaleb L.) genotypes. Pajouhesh & Sazandegi, 79: 54-61. (In persian)
-      Hazarika, B.N., and Bora, A., 2010. Hyperhydricity- A bottleneck to micropropagation of plants. Acta Horticulturae, 865: 95-101.
-      Ivanova, M. and Van Staden, J., 2008. Effect of ammonium ions and cytokinins on hyperhydricity and multiplication rate of in vitro regenerated shoots of Aloe polyphylla. Plant Cell, Tissue and Organ Culture 92: 227-231.
-      Jazirie, M.H. and Ebrahimi Rastaghi, M., 2003. Zagros Silviculture. Tehran University Publications, Tehran, 560 pp.
-      Kadota, M., and Nimi, Y., 2003. Effect of cytokinin types and their concentrationon shoot proliferation and hyperhydicity in in vitro pear cultivar shoots. Plant Cell, Tissue and Organ Culture, 72: 261-265.
-      Khatamsaz, M., 1992. Flora of Iran: Rosaceae. Research Institute of Forests and Rangelands. Tehran, pp: 241-267. (In persian)
-      Kollmann, J. and Pflugshaupt, K., 2005. Population structure of a freshly-fruited species at its range edge - the case of Prunus mahaleb L. in northern Switzerland. Botanica Helvetica, 115:49-61.
-      Lloyd, G. and McCown, B., 1981. Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by the use of shoot tip culture. Proceeding of Plant Propagation society, 30: 421-427.
-      Mahdaviyan, M., Bouzari, N. and Abdollahi, H., 2010. Effects of culture media and growth regulators on proliferation and rooting of a vegetative mahaleb rootstock (SL-64). Seed and Plant Improvement Journal, 1-26: 15-26. (In persian)
-      Mohseniazar, M., Nazeri, S., Malboobi, M.A., Ghadimzadeh, M., Barzgari, A. and Farokhzad, A., 2012. Optimizing factors affecting Agrobacterium tumefaciens mediated transformation of dwarf rootstock of apple (Malus domestica Borkh Cv Gami Almasi). Agricultural Biotechnology, 10: 7-15.
-      Murai,Y., Harada, H. and Yamashita, H., 1997. In vitro propagation of apricot (Prunus armenica L.) cv. 'BakuohJunkyou'. Journal of the Japanese Society for Horticultural Science, 66: 475-480.
-      Murashig, T. and Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiology Plant, 15: 473-497.
-      Nas, M.N., Gokbunar, L., Sevgin, N., Aydemir, M., Dagli, M. and Susluoglu, Z., 2012. Micropropagationof mature Crataegus aronia L., a medicinal and ornamental plant with rootstock potential for pome fruit. Journal of Plant Growth Regulation,67: 57-63.
-      Nezami, A.E., Garoosi, G.A., Haddad, R. and Babaei, M., 2010. Study the effects of pectin, medium type and plant growth regulators (PGRs) on micropropagation of GF677 under in vitro conditions. Quarterly Journal of Agricultural Biotechnology, 2: 113-126. (In persian)
-      Pati, P.K., Rath, S.P., Sharma, M., Sood, A. and Ahuja, P.S., 2006. In vitro propagation of rose-a review. Biotechnology Advances, 24: 94-114.
-      Ruzic, D.j.V. and Vujovic, T.I., 2008. The effects of cytokinin types and their concentration on in vitro multiplication of sweet cherry cv. Lapins (Prunus avium L.). HortScience,3: 12–21.
-      Sabeti, H., 2008. Forests, Trees, and Shrubs of Iran. Yazd University Publication, 886 pp. (In persian)
-      Sadeghi, F., Yadollahi, A., Jafarkhani Kermani, M. and Eftekhari, M., 2015. Optimizing culture media for in vitro proliferation and rooting of  Tetra (Prunus empyrean 3) rootstock. Journal of Genetic Engineering and Biotechnology, 13: 19–23.
-      Sedlák, J. and Paprštein, F., 2008. In vitro shoot proliferation of sweet cherry cultivars Karešova and rivan, Horticultural Science (Prague), 35 (3): 95–98.
-      Wu, H., Yu, X., Teixeira da Silva, J.A. and Lu, G., 2011. Direct shoot induction of Paeonia lactiflora ‘Zhong Sheng Fen’ and rejuvenation of hyperhydric shoots. New Zealand Journal of Crop and Horticultural Science, 39: 271-278.
-      Yildirim, H., Onay, A., Tilkat, E. and Akturk, Z., 2011. Micropropagation of the apricot (Prunus armeniaca L.) cv. Hacıhaliloğlu by means of single node culture. Turkish Journal of Agriculture and Forestry, 35: 55-64.
-      Zarei, M., Garoosi, G.h., Nezami, E., Hosseini, R. and Ahmadi, J., 2013. The effect of medium, carbon source, light spectrum and style treatment of auxin on shoot and root regeneration of gisela 6 rootstock. Journal of Cell & Tissue, 4: 169-185. (In persian)
Zou, Y.N., 2010. Micropropagation of Chinese Plum (Prunus salicina Lindl.) using mature stem segments. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38: 214-218
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Volume 24, Issue 1
May 2016
Pages 1-12

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