Effects of medium, genotype and plant growth regulators on micro  propagation of Jatropha curcas

Emam, Ldjvh; Mirjani, Leila; Naraghi, Tayebeh-sheila; Keneshlo, Hashem

doi:10.22092/ijrfpbgr.2015.102242

Effects of medium, genotype and plant growth regulators on micro propagation of Jatropha curcas

Document Type : Research Paper

Authors

10.22092/ijrfpbgr.2015.102242

Abstract

Jatropha curcas is a tropical fast growing tree from euphorbiaceae family. The valuable species has medicinal, industrial and ornamental applications. Regeneration of the species was performed by seeds and cuttings. Regarding Jatropha^'s seed nature (oily seed), for which low germination is resulted, regeneration of the species was studied by stem cuttings. For micro propagation, apical buds of 2 and 4 years old genotypes̉̉̉ (Gen 1 and Gen 2) from different seasons were applied. The best treatment of sterilization was found to be HgCl₂ 0.1% solution for 10 minutes on fall samples for genotype 2. The best medium for shoot proliferation was MS medium with BA (3 mg/l)and IBA (0.1 mg/l). Effect of genotype on shooting was nonsense but genotype 2 had higher effect on growth factors. The most rooting of shoots was observed in modified MS medium supplemented with 1 mg/l of IBA. Plantlets were acclimated in greenhouse after transferring to soil.

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