شناسنامه علمی، دوره 26 ، شماره 2، پاییز و زمستان 1397
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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
v.
2
no.
1397
https://ijrfpbgr.areeo.ac.ir/article_117952_4deb1130ab62d5ce4dd8e31601adb1a1.pdf
dx.doi.org/10.22092/ijrfpbgr.1397.117952
Identification of miRNAs and their target genes in red clover (Trifolium pretense)
M. R.
Naghavi
Corresponding author, Prof., College of Agriculture and Natural Resources, University of Tehran, Karaj, I.R.Iran
Email: mnaghavi@ut.ac.ir
author
A. A.
Karimi
M.Sc. student, College of Agriculture and Natural Resources, University of Tehran, Karaj, I.R.Iran
author
text
article
2018
per
DOR: 98.1000/1735-0891.1397.2.155.52.26.1588.41
MicroRNAs (miRNAs) are a group of 18_22 nucleotides long noncoding small endogenousthat derived from its precursor sequence and evolutionary conserved post-transcriptionalregulatory RNAs, which show an enormous role in various biological and metabolic processesin both animals and plants. MiRNA detection methods including microarray, qRT-PCR ,northern blot and bioinformatics methods in which the easiest and cheapest way to identifymiRNA is bioinformatics method. A bioinformatics approach was used to identify potentialmiRNA in red clover. EST-based homology search was applied to find potential miRNA of redclover. We blasted publicly available EST sequences obtained from NCBI GenBank againstpreviously known plant miRNAs. A total of six miRNA target genes based on theircomplementary sequences were also detected. Target genes encoding mono dehydro ascorbatereductase 1 play an important role in maintaining balance in the cycle of reactive oxygenspecies ascorbate-glutathione, FES1 proteins that contain zinc finger domain binding DNA,RHF2A gene encoding E3 ubiquitin-protein ligase involved in the positive regulation of thegametogenesis progression, RNA helicase ATP-dependent is involved in the RNA repair andmetabolism, Major facilitator superfamily (MFS) is a superfamily of membrane transportproteins that facilitate movement of small solutes across cell membranes in response tochemiosmotic gradients, AT-hook DNA binding motif protein.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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2018
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https://ijrfpbgr.areeo.ac.ir/article_117954_41604d4dcb5b96f364a70df8e6f6185c.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.117954
Isolation, characterization and expression analysis of 3-hydroxy-N-methylcoclaurine 4′–methyltransferase (4'OMT2) gene in Papaver somniferum L.
S. M.
Sohrabi
Ph.D. Student, Faculty of Agriculture, Lorestan University, Khorramabad, I.R.Iran.
author
A.
Ismaili
Corresponding author, Assos. Prof., Department of Agronomy and Plant Breeding, College of Agriculture, Lorestan University,
Khorramabad, I.R.Iran. E-mail: ismaili.a@lu.ac.ir
author
F.
Nazarian Firouz-Abadi
Prof., Faculty of Agriculture, Lorestan University, Khorramabad, I.R.Iran.
author
K.
Samiei
Ph.D. Student, Faculty of Agriculture, Lorestan University, Khorramabad, I.R.Iran.
author
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article
2018
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DOR: 98.1000/1735-0891.1397.2.165.52.26.1580.32
Poppy (Papaver somniferum L.) is one of the oldest medicinal plant in the world. It producesseveral important narcotic benzylisoquinoline alkaloids. In this study, 4’OMT2 (3′-hydroxy-Nmethylcoclaurine4′-O-methyltransferase) gene was isolated, sequenced and characterized. Afteramplification of genomic and coding sequence of 4’OMT2 gene using PCR, the fragments werecloned into pTZ57R/T plasmid and sequenced. Sequencing results showed two fragments of1074 and 1189bp for coding and genomic sequences, respectively. For the first time, resultsshowed one intron of 115bp in the genomic sequence of 4’OMT2 in the species. Isolated genefrom Iranian genotypes was similar to Papaver genus with high identity, and had 67% identityto Ranunculales plants. Further bioinformatics analyses revealed, 4’OMT2 gene produces astable enzyme without signal peptide that localize in the cytoplasm. For gene expressionanalysis, five poppy RNA-seq (mRNA type) libraries (belonging to flower bud, leaf, developingfruit, stem and root tissues) were retrieved from SRA database and analysis of 4’OMT2 geneexpression were done using IDEG6 software and statistical tests. Results showed that 4’OMT2has differential expression among different tissues and has maximum expression in stem tissues.Identification and characterization of biosynthetic genes is the first step in genetic manipulationand metabolic engineering. In present study, for the first time, full length coding sequence of4’OMT2 gene was isolated from poppy plant and presence of one intron in its sequence wasdetermined.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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2
no.
2018
165
176
https://ijrfpbgr.areeo.ac.ir/article_117956_c7b76428124b6e8d0f6988b50a4e6f31.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.117956
Analysis of genetic diversity of Pistacia atlantica Desf. populations from Zagros forests using ISSR, IRAP and SCoT molecular markers
H.
Shah-Ghobadi
M.Sc. Graduate, Forest Ecology and Silviculture, Faculty of Natural Resources, University of Kurdistan, I.R.Iran
author
N.
Shabanian
Corresponding author: Assoc. Prof., Faculty of Natural Resources, University of Kurdistan, I.R.Iran
E-mail: n.shabanian@uok.ac.com
author
A.
Khadivi
Assoc. Prof., Horticultural Sciences Department, Faculty of Agriculture and Natural Resources, Arak University , I.R.Iran
author
M. S.
Rahmani
Ph.D Student in Plant Biotechnology, Faculty of Agriculture and Natural Resources, Department of Biotechnology, University of
Tehran, I.R.Iran
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DOR: 98.1000/1735-0891.1397.2.177.52.26.1605.84 This study was conducted to investigate intra- and inter-population genetic diversity of 15populations of Pistacia atlantica from Zagros forests, represented by 181 genotypes, using 15,12 and 13 primers based on three molecular marker including inter simple sequence repeats(ISSR), inter retrotransposal amplified polymorphism (IRAP) and start codon targeted (SCoT)markers, respectively. ISSR, IRAP and SCoT primers produced a total of 186, 143 and 136bands, of which 162 (87.0%), 130 (90.9%) and 120 (88.0%) showed polymorphisom,respectively. Results showed that all marker systems revealed relatively high genetic variationat population level (hISSR = 0.22, PPLISSR = 61.92%; hIRAP = 0.25, PPLIRAP = 74.19%; hSCoT =0.20, PPLSCoT = 64.09%). According to ISSR, IRAP and SCoT based genetic differentiationcoefficient (ΦST; 0.28, 0.23, and 0.22, respectively), a higher level of genetic variation wasrecorded within populations than that of among populations, that could be due to wind-basedextensive gene flow among the populations. UPGMA cluster analysis based on three usedmolecular systems, devided the studied populations into distinct clusters. According to Manteltest, no significant correlation was recorded between genetic and geographic distances of thepopulations.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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177
195
https://ijrfpbgr.areeo.ac.ir/article_117957_d68dbcb72dc9e66ff76fe47035996f48.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.117957
Genetic diversity of several Iranian sumac (Rhus coriaria) populations using genomic inter-microsatellites markers
R.
Mohammadi Alaghoz
M.Sc., Plant Breeding, Department of Plant Breeding and Biotechnology, Urmia University, Urmia, I. R. Iran.
author
R.
Darvish zadeh
Corresponding author, Prof., Department of Plant Breeding and Biotechnology, Urmia University, Urmia, I.R. Iran.
Email: r.darvishzadeh@urmia.ac.ir
author
A.
Alijanpour
Assoc. Prof., Department of Forestry, Faculty of Natural Resources, Urmia University, Urmia, I. R. Iran.
author
H.
Hatami Maleki
Assist. Prof., Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, Maragheh, I. R. Iran.
author
R.
Heidari
M.Sc., Agricultural Biotechnology, Institute of Biotechnology, Urmia University, Urmia, I. R. Iran.
author
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2018
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DOR: 98.1000/1735-0891.1397.2.196.52.26.1576.82
Sumac (Rhus coriaria L.) as a forest shrub has been dispersed in Mediterranean and EasternAsia specially Iran and has several medicinal and industrial applications. In this research, intermicrosatellitesmarkers were used to study genetic diversity of sumac genotypes collected fromfive natural habitats located in North West of Iran (West and East Azerbaijan provinces).Fifteen samples were select randomly from each population and genomic DNA wasfingerprinted by 18 ISSR markers. Results revealed existence of suitable genetic variationamong the studied sumac genotypes and portion of inter population (79.8%) variation werehigher than intra population (20.2%) variation. Minimum value of genetic distance (14.18) wasobserved between Aghberaz-Horand (East Azerbaijan province) and Nir-Arasbaran (EastAzerbaijan province) populations and the maximum one (31.08) was observed betweenKachelleh-Urmia (West Azerbaijan) and Nir-Arasbaran populations. According to classificationof genotypes based on molecular data, Aghberaz-Horand and Nir-Arasbaran populations locatedin the same group and populations from other habits located in distinguished groups.Classification of genotypes via ISSR markers was in agreement with their geographicaldistributions. ISSR markers could be effectively applicable in genetic evaluation of sumacgermplasm and also in identification of genetic relatedness among known and unknown sumacsamples.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
v.
2
no.
2018
196
206
https://ijrfpbgr.areeo.ac.ir/article_117959_7a1b7fee4022449ac9015b9410eceeac.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.117959
Application of SCoT marker to discriminate Lolium perenne and Lolium multiflorum species
M.
Farshadfar
Corresponding author, Assoc. Prof., Department of Agriculture, Payame Noor University, I.R. Iran
Email: farshadfarmohsen@yahoo.com
author
H.
Shirvani
Instructor, Department of Agriculture, Payame Noor University, I.R. Iran
author
M.
Amjadian
Department of Agriculture, Payame Noor University, I.R. Iran
author
A.
Yaghotipoor
Ph.D in Plant breeding, Department of Agronomy and Plant breeding, Faculty of Science and Agriculture Engineering, Razi
University, Kermanshah, I.R. Iran
author
text
article
2018
per
DOR: 98.1000/1735-0891.1397.2.207.52.26.1603.109
Lolium is an important cool season grass species growing in different climates. To identifyvariation among nine Lolium multiflorum and nine Lolium perenne genotypes, this experimentwas carried out based on 15 SCoT molecular markers. All of the SCoT primers showed 86visible bands. Seventy four out of 86 bands were polymorphs. Primers SC35, SC36 and SC26(with 9 and 8 bands respectively) showed the highest number of bands and SC10 and SC44(with 2 and 3 bands) showed the lowest number of bands. The greatest number of band forpolymorphic information content (PIC) belonged to SC5، SC40، SC35 ،SC63 ،SC13, C44،SC26 ،SC21and SC10. The greatest Resolving Power (RP), Marker Index (MI), EffectiveMultiplex Ratio (EMR) belonged to SC35 andSC26 primers. Genetic similarity based onJaccard coefficient ranged from 0.35 to 0.91. Mean similarity between the genotypes was 0.64.Cluster analysis grouped genotypes into two groups. The results of clustering was confirmed byprincipal coordinate (PCo) analysis. Analysis of molecular variance of annual and perennialspecies revealed that variation within species was 51% and variation between species was 49%.Based on heterogeneity (He) and Shannon indices (I), the highest intraspecific variability andgene variation belonged to L. Multiflorum.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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2
no.
2018
207
220
https://ijrfpbgr.areeo.ac.ir/article_117960_4b0c88d6fbb8b2011ec8dec96abe1b86.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.117960
Investigating morphological characteristics and genetic relationships between two populations of Ferula pseudalliacea in Yazd Province
S.
Hossein Jafari
Corresponding author, PhD Student, Rangeland Science, Department of Natural Resources, Agricultural Science and Natural
Resources University of Gorgan, Gorgan, I.R. Iran. Email: Samirahosseinjafari@yahoo.com
author
A.
Sepehry
Prof., Department of Range Management, Faculty of Natural Resources, Agricultural Science and Natural Resources University of
Gorgan, Gorgan, I.R. Iran.
author
H.
Soltanloo
Associ. Prof., Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Agricultural Science and Natural
Resources University of Gorgan, Gorgan, I.R. Iran.
author
A. A.
Karimian
Associ. Prof., Department of Range and Water Management, Faculty of Natural Resources and Desert Study, Yazd University,
Yazd, I. R. Iran.
author
text
article
2018
per
DOR: 98.1000/1735-0891.1397.2.221.52.26.1576.32
Ferula pseudalliacea (bitter assafoetida) is a rangeland plant which its gum has a lot ofmedicinal properties. Since there is no report about evaluating genetic diversity of the valuablespecies in Iran, this is the first study to investigate genetic diversity of the species in its habitatsin Yazd province using several morphological traits and ISSR markers. Among 9 traits, therewas significant differences between habitats in terms of canopy cover, stem diameter and weightof 1000 seed. Cluster analysis by morphological traits on the basis of Ward's method classifiedthe samples into three main groups. Among 22 primers, 8 primers reproduced the pattern DNAwell and revealed 224 sharp bands. ISSR-16 and ISSR-55 primers had better performanceaccording to the number of bands, PIC and Marker Index. Cluster analysis using Jacquard'scoefficient and UPGMA method, grouped two populations into 10 clusters. Principalcoordinates analysis showed three components explained 57.94 percent of total variance andseparated the populations from each other. These grouping matched relatively with thegeographical regions. AMOVA showed that within group genetic diversity (92%) was morethan between group diversity (8%). Comparing morphological and molecular dendrogramsrevealed that there was no similarity between them. The results showed that ISSR marker wassuitable to investigate genetic diversity among the studied populations of the species.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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2
no.
2018
221
232
https://ijrfpbgr.areeo.ac.ir/article_117961_b13a077c4fe01b3e422dd0ee57764768.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.117961
Tissue culture and organogenesis of Iranian mountain ash (Sorbus persica Hedl.)
M.
Esmaeili Sharif
Corresponding author, Assist Prof., Research Division of Natural Resources, Isfahan Agricultural and Natural Resources
Research and Education Center, Agricultural Research, Education and Extension Organization, AREEO, Isfahan, I.R.Iran
author
S. M.
Hosseini Nasr
Assoc. Prof. Department of Forestry, Faculty of Natural Resources, Sari Agricultural Sciences and Natural Resources University
(SANRU), Sari, I.R. Iran.
author
A.
Ghamari Zare
3-Assoc. Prof., Biotechnology Research Division, Research Institute of Forests and Rangelands, Education and Extension
Organization, AREEO, Tehran, I.R. Iran.
author
M.
Talebi
Assoc. Prof., Biotechnology Department, College of Agriculture, Isfahan University of Technology, Isfahan, I.R. Iran.
author
text
article
2018
per
DOR: 98.1000/1735-0891.1397.2.233.52.26.1605.32
Iranian mountain ash (Sorbus persica Hedl.) is one of the endangered species with greatvalues in terms of soil conservation, climate stratification, medicinal properties, high resistanceto frost, and importance of habitat for wildlife. Present research was conducted with the aim ofachieving to an appropriate method of micropropagation of the species. Nodal segments weretaken from stem axis of in vitro elongated plants. The segments were cultured on WPMmedium, supplemented with 27 hormonal composition of BAP, IBA and TDZ. Then theresulting shoots were transferred to a media supplemented with various concentrations of IBAor NAA for rooting induction, and finally they were moved to a greenhouse. Percentages ofregeneration of the explants were 98 -100%. The highest number of shoots was obtained byTDZ (0.23 μM) + IBA (0.1 μM) and BA (4.4 μM), the highest shoot length (5.2 cm) wasobtained by TDZ (0.0 μM) + IBA (0.0 μM) and BA (4.4 μM) and the highest number of nodes(8) was achieved by TDZ (0.23 μM) + IBA (0.0 and 0.1 μM) and BA (2.2 and 4.4 μM). Therewas a significant difference between IBA concentrations in different treatments for rootingpercentage. Maximum rooting percentage was 14.7% in IBA concentration of 5 μm. The rootedsegments were successfully acclimated to greenhouse conditions. Results of the experimentshowed that in the absence of BAP hormone, umber of shoots produced by the segmentssignificantly were decreased for all of the tested treatments. Fifteen percent of the transferredexplants to the rooting media produced one to three roots per explant.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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2018
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https://ijrfpbgr.areeo.ac.ir/article_117962_4cc7d1e9214fa2196cd1143db7d69817.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.117962
Assessment of NAC2, MYB and CBF14 genes expression in susceptible and resistant Aegilops genotypes to salinity
A.
Mahmoudi
Former M.Sc. student of Biotechnology, Dep. of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of
Guilan, Rasht, I.R.Iran
author
A.
Aalami
Corresponding author, Assoc. Prof., Dep. of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan,
Rasht, I.R.Iran. Email: ali_aalami@guilan.ac.ir
author
H.
Hasani Komleh
Assist. Prof., Dep. of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, I.R.Iran.
author
M.
Esfehani
Prof., Dep. of Agronomy and plant breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, I.R.Iran
author
R.
Shirzadian
Assist. Prof., Dep. of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, I.R.Iran.
author
text
article
2018
per
DOR: 98.1000/1735-0891.1397.2.244.52.26.1575.41
Salinity is one of the main environmental factors limiting production and distribution ofplants worldwide. Aegilops is a wild species from grasses family with useful genes such asenvironmental stress tolerance. Two genotypes, 575 from Aegilop cylindrica as resistant and675 from Aegilop crassa as sensitive to salinity were used base on previous experiments insalinity stress. After seed germination, the seedlings were transferred to a sandy environmentunder controlled light and temperature conditions and irrigation was performed under twonormal conditions and 200 mM salinity with Hoagland solution. Leaf tissue samples were takenat 0, 6, 24, 48 and 72 hours after salinity treatments. After extracting the RNA and the cDNAsynthesis, its accuracy was verified by 18S rRNA. The expression of the three transcriptionfactors of CBF14, NAC2 and MYB was investigated by using Real Time-PCR. Results showedthat NAC2 and CBF14 transcription factors had the highest expression at 48 hours after salinitystress, but the MYB gene had the highest expression at 24 hours after stress. Overall, theexpression of CBF14, NAC2, and MYB genes in the 575 genotype compared with genotype 675was in higher level in all hours. The results showed that the three mentioned genes playimportant role in salt tolerance in the tolerant genotype, therefore, considering the high geneticsimilarity between Aegilops and wheat, the genes can be as candidate genes for using in wheatbreeding programs.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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2018
244
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https://ijrfpbgr.areeo.ac.ir/article_117963_be66269a3d340d5c5d853398c966c136.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.117963
Evaluation of genetic diversity effects on morpho-physiological and antioxidant responses in different species of Aegilops under drought stress
S.
Fabriki-Ourang
Assistant Professor, Department of Production and Plant Breeding, Imam Khomeini International University, Qazvin, Iran
author
B.
Shahidi
M.Sc. Student, Imam Khomeini International University, Qazvin, I.R.Iran
author
text
article
2018
per
DOR: 98.1000/1735-0891.1397.2.254.52.26.1605.1610
This experiment was conducted to investigate the effect of drought stress on photosyntheticpigments contents, biomass of roots and shoots, total protein content and antioxidant enzymesactivity and non-enzymatic antioxidant of six species of Aegilops genus along with two droughtresistant and susceptible wheat cultivars as checks in a factorial based on randomized completeblock design with three replications. Drought stress treatments were applied at three levels ofnon-stress (FC=100%), moderate stress (FC=50%) and severe stress (FC=25%) conditions. Ae.caudata, Ae. triuncialis and Ae. cylindrica were ranked higher than resistance cultivar, Sirvan,as check in term of root fresh and dry weights under stresses. The highest total chlorophyllcontent was related to Ae. cylindrica, Ae. umbellulata and Ae. caudata in stresses. Carotenoidshowed decreasing trend in all studied species except for Ae. umbellulata, and Ae cylindrica hadthe highest amount at both stress and non- stress conditions. The highest SOD under severestress was observed in Ae. crassa and Sirvan as resistant cultivar. Maximum APX under severestress was observed in Ae. triuncialis and Ae. caudata and the lowest activity was in Ae.neglecta. The highest level of GPX was observed in Ae. triuncialis, Ae. caudata and Sirvancultivar under drought stress. Also the maximum CAT enzyme under severe stress wasobserved in Ae. triuncialis, Ae. caudata and Ae. crassa, respectively, and Ae. umbellulata andDarya cultivar had the lowest amount of CAT enzyme. In conclusion, because the three speciesAe. caudata, Ae. triuncialis and Ae. cylindrica had the highest amounts for the traits associatedwith increased drought tolerance, including root volume, carotenoid, CAT, GPX and APXenzymes, and also these species were same in having CC genome. Therefore, it seems that theCC genome could have a high potential for future wheat breeding programs in increasing ofdrought tolerance.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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2
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2018
254
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https://ijrfpbgr.areeo.ac.ir/article_117964_f25a0da0831ccadabff5bb6c3382c36c.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.117964
Genetic variability of Tanacetum polycephalum populations in West Azarbaijan using ISSR molecular markers
M.
Mojarrad Ashenaabad
PhD Student, Department of Biology, Faculty of Science, Urmia University Pardis, Urmia University, Urmia, I.R. Iran.
Email: Mojarrad2017@gmail.com
author
S.
Hosseini Sarghein
Corresponding author, Assist. Prof, Department of Biology, Faculty of Science, Urmia University, Urmia, I.R. Iran.
author
A.
Sonboli
Assoc. Prof., Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, I.R.
Iran.
author
R.
Heidari
Prof., Saba Nonprofit University, Urmia, I.R. Iran.
author
text
article
2018
per
DOR: 98.1000/1735-0891.1397.2.268.52.26.1576.82
Tanacetum polycephalum from Anthemideae tribe of Asteraceae family is a rangelandspecies with high morphological diversity and vast dispersal in Northwest, East and centre partsof Iran. The purpose of this research was studying genetic diversity of 20 populations of T.polycephalum in West Azarbayjan using ISSR molecular marker. Ten ISSR primers out of 14primers could be scored based on presence or absence of each band. Over all 208 bands wereproduced .The range number of bands was between 15(IS24) to 28 (IS20) and the average was20.8. Regarding the polymorphism bands percentage, IS8, IS1, IS10, IS13, IS20 markers hadhigh percentage (100) and IS24 marker had the lower percentage (80) and mean percentage ofpolymorphism of the primers was %96.03. PIC, RP, EMR and MI means were 0.30, 6.06, 19.5and 6.13 respectively. Over all the most effective markers were IS8, IS1 and IS10. Results ofcluster analysis revealed the grouping of studied populations in 2 main and 4 subgroups, inwhich the different populations collected from the same locality were located in the same group.Therefore, a close relationship was characterized between geographic location and geneticdiversity and it may be concluded that geographic and climatic factors could be one of theresponsible main factor for genetic diversity.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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2
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2018
268
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https://ijrfpbgr.areeo.ac.ir/article_117965_10652a3129b1962d4ded257818710c52.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.117965
Ecotypic diversity alteration of Quercus castaneifolia populations in different elevation classes of forest (case study: East of Golestan province)
A.
Ahmadi
M.Sc., Graduated Student of Silviculture and Forest Ecology, Forest Science Faculty, Gorgan University of Agricultural Sciences
and Natural Resources, Gorgan, I.R.Iran.
author
D.
Azadfar
Corresponding Author, Assoc. Prof., in Forest Biotechnology, Forest Science Faculty, Gorgan University of Agricultural
Sciences and Natural Resources, Gorgan, I.R.Iran, Email: azadfar.d@gmail.com
author
Z.
Saeedi
Ph.D. in Forest Sceince, Forest Science Faculty, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan,
I.R.Iran.
author
text
article
2018
per
DOR: 98.1000/1735-0891.1397.2.279.52.26.1578.41
Knowledge and understanding the genetic diversity of species is very important foradaptation to environmental changes and their long-term survival and also for the managementof forest genetic resources conservation. Therefore, study of industrial or ecologically importantspecies should be done in order of priority. The aim of this research was exploring the ecotypicdiversity changes of Quercus castaneifolia populations in elevation classes of Golestan province(low, middle, and upper elevation) by the qualitative study of peroxidase biochemical marker inthe biennial branches of six different populations. Qualitative study of peroxidase marker wasdone by vertical electrophoresis and PAGE method (Poly Acrylamide Gel Electrophoresis).Based on the results, populations of altitudinal classes were different regarding amount ofdiversity and ecotypes were separable from each other. Therefore, index bases in the seedproduction areas should be determined and their natural regeneration should be increased andgene flow should be improved through the seeds of index bases of the adjacent populations withsimilar ecological conditions with controlling the number of selected index bases consideringthe increased diversity in each habitat.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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2018
279
291
https://ijrfpbgr.areeo.ac.ir/article_117966_cf257ef97459e23e376d257412ba35a4.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.117966
The study of genetic diversity of cumin ecotypes of Khorasan provinces using protein markers
M.
Zabet
Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Birjand
author
A.
Rahimi
Assoc. Prof., Department of Agronomy and Plant Breeding, College of Agriculture, University of Birjand, I.R.Iran
author
A.
Izanloo
Assist. Prof., Department of Agronomy and Plant Breeding, College of Agriculture, University of Birjand, I.R.Iran
author
Z.
Alizadeh
Assist. Prof., Department of Agronomy and Plant Breeding, College of Agriculture, University of Birjand, I.R.Iran
author
text
article
2018
per
DOR: 98.1000/1735-0891.1397.2.292.52.26.1576.1610
Cuminum cyminum is one of the oldest and most economically important species in theApiaceae family that has the highest cultivated areas of Khorasan provinces in Iran. Geneticdiversity of 20 ecotypes of Cuminum cyminum was investigated by protein markers. Theecotypes were randomly collected from three provinces of North, Razavi and South Khorasanprovinces. Protein profile analysis was carried out by SDS-PAGE method. The methodincluded: extracting protein from the sample seeds, determining samples concentration, andfinally running on electrophoresis gel. Analysis of molecular variance showed that there wasvariation between and within the populations. Based on the protein markers %24 of thevariations belonged to between populations and %76 belonged to within populations.Polymorphism percentage, polymorphic content mean, Shannon's information index and theaverage of Nei's information index were 27%, 0.01, 0.49, and 0.68, respectively. Results ofPCoA showed that the first three components accounted for 83% of the variation. Clusteranalysis based on the similarity matrix classified the studied ecotypes in 5 clusters.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
v.
2
no.
2018
292
301
https://ijrfpbgr.areeo.ac.ir/article_117967_68de00bef8e2ebb1769ef244b6ad7772.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.117967
Diallel analyses for dry forage yield among some alfalfa cultivars
I.
Bernousi
Assoc. Prof., Department Plant Breeding and Biotechnology, Agricultural Faculty, Urmia University, Urmia,
I.R.Iran Email: i.bernosi@urmia.ac.ir
author
A.
Hasanzade Ghorttappe
Assist. Prof., Center of Agricultural Research and Natural Resources of West Azerbaijan, Agricultural Research,
Education and Extension Organization (AREEO), Urmia, I.R. Iran
author
text
article
2018
per
DOR: 98.1000/1735-0891.1397.2.302.52.26.1578.1607
The objective of this study was to develop population hybrids by crossing five alfalfacultivars of different geographic origin (Ghareh Yonjeh, Hamedani and Mahalie-Esfahan fromIran, Elchi from Turkey and Ordobad from Azerbaijan) and assessment of combining abilityamong them using diallel analysis. A half diallel was performed during 2012 between theselected cultivars. For each pairwise cross, ten plants were chosen at random to obtain F1generation. Heterotic responses were determined by evaluation forage yield of the cultivars andtheir 10 half-diallel hybrids in seeded plots that were harvested three times in each of 2 years(2014 and 2015). Variation among crosses was attributed primarily to general combining ability(GCA) effects. However, specific combining ability (SCA) effects were also significant. Midparentheterosis ranged from 7.1% in the Elchi x Hamedani to -9.9 % in the Ghareh Yonjeh xMahalie-Esfahan and high-parent heterosis ranged from 3.4 % in the Elchi x Hamedani to 16.7%Ghareh Yonjeh x Mahalie-Esfahan. Results indicated that Elchi cultivar could beconsidered as a member of a potential heterotic group for adapted cultivars (Ghareh Yonjeh andHamedani) to the studied environment.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
v.
2
no.
2018
302
310
https://ijrfpbgr.areeo.ac.ir/article_117977_7e9fbb117e4a5d4e9442a190dbc45686.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.117977
Evaluation of yield and quality traits of tolerant and semi tolerant populations to powdery mildew in sainfoin (Onobrychis viciaefolia)
M.A.
Alizadeh
Corresponding author, Assoc. Prof., Research Institute of Forests and Rangelands, Agricultural Research Education and
extension Organization (AREEO), Tehran, I.R. Iran
E-mail: Alizadeh202003@gmail.com
author
A. A.
Jafari
Prof., Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO),
Tehran, I.R. Iran
author
S. E.
Sayedian
Researcher, Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization
(AREEO), Tehran, I.R. Iran
author
M.
Amirkhani
Researcher, Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization
(AREEO), Tehran, I.R. Iran
author
M. R.
Pahlevani
Researcher, Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization
(AREEO), Tehran, I.R. Iran
author
L.
Fallah Hoseini
Researcher, Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization
(AREEO), Tehran, I.R. Iran
author
M.
Ramezani Yeganeh
Researcher, Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization
(AREEO), Tehran, I.R. Iran
author
text
article
2018
per
DOR: 98.1000/1735-0891.1397.2.311.52.26.1575.41
In order to identify tolerated populations of sainfoin to powdery mildew, seeds of tenpopulations of Onobrychis viciaefolia, originated from different regions of Iran were sown inAlborz research station during 2013. Tolerant and susceptible populations were cultivated innatural condition and evaluated in terms of contamination and other parameters as yield andquality traits. Result showed that two populations of 15353, 3001, Oshnaviaeh and Polycrosshad disease severity index less than 25% and nominated as tolerant populations. The rest ofpopulations due to disease severity index between 25 to 50% and over than 50% wereconsidered as semi tolerant and susceptible, respectively. The tolerant (15353, 3001,Oshnaviaeh and Polycross) populations had higher forage and seed yield than susceptiblepopulations. They showed also higher values of quality traits (Protein content, water solublecarbohydrate, digestive material and ash content) than susceptible ones. In contrast thesusceptible populations had higher crude fiber, ADF and NDF compared with tolerantpopulations. In principle component analysis (PCA), the first four components accounted for83% of total variation. The first three components were known as yield, quality traits anddisease severity index, respectively. Result of cluster analysis showed that population of 3001,15353, 9263, 4083 and Oshnaviaeh and Polycross were located in cluster 2 thatthese popolations have lower mean values of disease severity index and higher forage and seedproduction.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
v.
2
no.
2018
311
326
https://ijrfpbgr.areeo.ac.ir/article_117980_3f5b958e2ae028d09b436a99d8c6cfb7.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.117980