Relationship between RuBisCO and photosynthetic efficiency of tissue cultured planflets
S.
Karamzadeh
Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran,
Iran.
author
text
article
2002
per
In vitro plants show an abnormality in growth and photosyntheticperformance. In order to understand the role of the main photosyntheticenzyme (RuBisCO) in photosynthetic efficiency (Pm), and the factorsaffecting activity of this enzyme, an experiment was carried out. Theplantlets of wild clrerry were examined for some physiological parameters,under three growth conditions (in vitro, and ex vitro after 2 ar.td 4 weeks).The observations showed that under in vitro conditions Pm is too lowcompared to ex vitro conditions. changes in Pm of different treatmentswere consistent with changes in RuBisCO activity. No significantdifference was observed between leaf RuBisco content in vitro and exvitro. No changes in RuBisco activity were observed under differentgrowth irradiances (50 and 200 pmol photon --' .-t) in in vitro condition,while under ex vitro conditions higher RuBisCO activity was observedunder higher growth irradiance. Therefore, Pm of in vitro plantlets wasaffected by low activity of RuBisCO. As previously reported and the resultsof this study also support it, the low activity of this enzyme could beattributed to presence of sucrose in culture medium and to the lowconcentration of magnesium ions in leaf of in vitro plantlets.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
10
v.
1
no.
2002
1
14
https://ijrfpbgr.areeo.ac.ir/article_115679_18cc23f8881a663a79846616ddc515f8.pdf
dx.doi.org/10.22092/ijrfpbgr.2002.115679
Optimizing DNA extraction procedure in case of Amygdalus spp.
s.
Kadkhodaie
1 - Postgraduate student, Tabriz University
2 - Research Institute of Fortests and Rangelands, P.O.Box 13185-l16, Tehran
author
S.R.
Tabaie-Aghduie2
Research Institute of Fortests and Rangelands, P.O.Box 13185-l16, Tehran
author
text
article
2002
per
A simple method was developed to extract sufficient amount of genomicDNA, from plant species containing high amount of secondary metabolites.Leaves of different Amygdalus species including A. scoparia, A.haussknechtii, A. lyciodes, A. elaegnifolia and A. commonis were used forDNA extraction. SDS (instead of CTAB or Sarkosile), NaCl (to removepolysaccharides), PVP (to remove polyphenolic compounds) were used inextracting buffer. Sodium chloride was used again for more polysaccharideremoval and RNase to remove RNA. Also, extraction was repeated severaltimes, using chloform: isoamyl-alchohol to abtain more purified DNA.Average yield of DNA, extracted with this procedure was 50 pg per I gleaf tissue (70pglg and 30pg/g in young and old leaf tissues, respectively).Extracted DNAs showed successful reproducibility through PCRamplification. This method which is relativerly inexpensive, could beefficiently applied for other species of rosaceae or different plant familiesfrom which DNA extraction is cumbersome.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
10
v.
1
no.
2002
15
27
https://ijrfpbgr.areeo.ac.ir/article_115682_9a2498c1a848a6261ba008b6ad58a4fe.pdf
dx.doi.org/10.22092/ijrfpbgr.2002.115682
sexual propagation of Argania spinosa L. Skeels using ovary culture
A.
Jafari-Mofidabadi
Research Institute of Forests and Rangelands,Tehran,P.O.Box13185 -l l6'
author
A.
Eghtesadi
Research Institute of Forestsand Rangelands,Tehran,P.O.Box13185 -l l6'
author
text
article
2002
per
In order to propagate Argania spinosal. skeels, developed ovary culture were used. Seld wittr hard coat were sterilized with 70% of ethanol and rinsed with sterile water. Hard coat was aseptically removed and ovary was isolated. Two type of Isolated ovary (complete and cut ovary explants), were cultured intothe solidified MS, WPM and half-MS (half-concentrated MS rnedium) for embryo germination. Highest embryo germination was observed in cut ovary explant culture (63162%).In spite of no significant differences between media for embryo germination, MS medium produced highest embryo germination for both kind of explants (49152%)' Plantlets were successfully acclimatized and transferred to the greenhouse.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
10
v.
1
no.
2002
29
36
https://ijrfpbgr.areeo.ac.ir/article_115689_add1f71b6fd8e931f13d380d1b7b87d0.pdf
dx.doi.org/10.22092/ijrfpbgr.2002.115689
Effects of CaCO3 on alfalfa salinity tolerance
M.
Yarnia
Assist. Prof., Tabriz Azad Univ. Tabriz, Iran
author
H.
Heidari
Assoc. Prof., Research Institute of Forests and Rangelands, P. O. Box: 13185-116. Tehran, Iran
author
F.
Rahimzadeh Khoiy
Prof.,Univ of Tabriz, Tabriz, Iran.
author
text
article
2002
per
Alfalfa is one of the most important forage crop that is highly adaptivity to environment unfavorable conditions. High genetic diversity, yield quality and quantity have made alfalfa the most utilised crop in world. One of the most important factors which decreases yield and plantig area is soil and water salinity. Selection for tolerant genotypes with suitable planting management would lead to increase yield and cultivation area in saline conditions. Adding Ca'* to environment by its antagonistic effects on Na* leads to decrease toxiciticeffect of Na*. Therefore, Four cultivars namely Golestan (20313), Sistan and Bluchestan, F.A.O (2566) and Ahar Hamedani were arrangedin an experimental design to study the Ca2* effectson growth and yield in saline conditions. Experiment was conducted in RBCD based factoriel in 5 salinity levels in 3 replicates at hydroponic culture. The cultivars were grown in saline condition untill the firstcuffing and then the pots were leached with distilled water. Salinity and Ca'* levels were added in Hougland nutrient solution and applied the pots. Shoot, root, stem and leaf dry matter, biomass, plant height, main root length, stem internod numbers and tiller numbers per plant were measured Dry matter production has the rnost significant correlation with stem dry weight in salinity (R50.962). Using Ca" decreases the salinity effect on above factors, especially in resistant cultivar.Golestan (20313) and F.A.O (2566) cultivars had the most dry matter in Ca" and saline conditions.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
10
v.
1
no.
2002
38
55
https://ijrfpbgr.areeo.ac.ir/article_115698_e144ff4f6e966dfee06e4bae2163b33e.pdf
dx.doi.org/10.22092/ijrfpbgr.2002.115698
Hybridization between Secale cereale and Hordeum spantaneum
F.
Kazemi-saeed
Research Institute of Forests and Rangelands, P.O.Box: 13185-l 16, Tehran.
author
text
article
2002
per
In order to investigate the possibility of intergeneric hybrid production through artificial crossing between Hordeum spontaneum and Secale this study was done in Research Institute of Forests and Ranglands' Seeds of S. cereale were planted in pots. Before maturity of pollens and "rr"il, natural pollination, anthers were omitted to emasculate female plants' Pollen grains were gathered from H. spontaneum, then female parents were pollinat-ed with thern. Hybrid seeds were formed, and although they were wrinkled but they showed enough germination ability. The hybrid seeds were planted in pots and obtained plants were morphologically between two parents.The hybrid plants flowered, but because of abnonnality in meiosis devision, seed was not formed. Results therefore, indicated that by artificial crossing itrtergeneric hybrid seeds can be successfully obtained.However special treatments such as colchicine must be applied to get tetraploid fertile intergeneric hybrids.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
10
v.
1
no.
2002
57
68
https://ijrfpbgr.areeo.ac.ir/article_115701_699bf5f6dc0d2312cc1916c3f3ec543f.pdf
dx.doi.org/10.22092/ijrfpbgr.2002.115701
Asexual regeneration of Castaneu sutiva (chesnut) by shoot tip culture
T. S.
Naraghi
Research Institute of Forests and Ranglands, and Physilogy . P. O. Box: 13185 - I16, Tehran, Department Of Plant Genetics Iran.
author
text
article
2002
per
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
10
v.
1
no.
2002
69
89
https://ijrfpbgr.areeo.ac.ir/article_115787_e39a0a5c84d4aa3c672c119fde5091c4.pdf
dx.doi.org/10.22092/ijrfpbgr.2002.115787
Evaluation of seed yield and seed yield components in 29 accessions of cocksfoot (Dactylis glomerata)
A. A.
Jafari
Members of Scientific Board of Reaserch Institue of Forests and Rangelands, P. O. Bou 13185-l16, Tehran
author
A.
Bashirzadeh
Former Postgraduted Student of Islamic Azad University, Branch
author
H.
Heidari Sharifabad
Members of Scientific Board of Reaserch Institue of Forests and Rangelands, P. O. Bou 13185-l16, Tehran
author
text
article
2002
per
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
10
v.
1
no.
2002
91
129
https://ijrfpbgr.areeo.ac.ir/article_115788_7f14218dd3a21eb77806ec4f5be0b137.pdf
dx.doi.org/10.22092/ijrfpbgr.2002.115788
Mixoploidy and Aneuploidy in Lolium species
H.
Mirzaie-Nodoushan
Research Institute of Forests and Rangelands, p.o.Box 131g5-116.
author
H.
Nadarkhani
Payam Noor University, Tehran, Iran
author
text
article
2002
per
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
10
v.
1
no.
2002
131
144
https://ijrfpbgr.areeo.ac.ir/article_115789_bef7cae11691872e15362d281d5089c5.pdf
dx.doi.org/10.22092/ijrfpbgr.2002.115789