Contents, Vol. 26, No. (1), 2018
text
article
2018
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Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
v.
1
no.
2018
https://ijrfpbgr.areeo.ac.ir/article_116633_d55d40eb3c69c946850a791cbda8ddb2.pdf
dx.doi.org/10.22092/ijrfpbgr.1397.116633
Systematic knockdown of thebaine 6-O-demethylase gene in Papaver somniferum L., using virus-induced gene silencing technique
S.
Alipour Amraei
M.Sc., Department of Agronomy and Plant Breeding, College of Agriculture, Lorestan University, Khorramabad, I.R.Iran
author
A.
Ismaili
Corresponding author, Assos. Prof., Department of Agronomy and Plant Breeding, College of Agriculture, Lorestan University,
Khorramabad, I.R.Iran. E-mail: ismaili.a@lu.ac.ir
author
F.
Nazarian FirouzAbadi
Prof., Department of Agronomy and Plant Breeding, College of Agriculture, Lorestan University, Khorramabad, I.R.Iran
author
A.M.
Latifi
Assoc. Prof., Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, I.R.Iran
author
text
article
2018
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Papaver somniferum is one of the important commercial sources for several pharmaceuticalalkaloids including the narcotic analgesics codeine and morphinane and the semi-syntheticdrugs oxycodone, buprenorphine and naltrexone. One of the downstream genes in alkaloidbiosynthesis pathway in the species is thebaine-6-O demetylase (T6ODM) that inverts thebaineand oripavine to codeinone and morphinone, respectively. In this study, a transient silencingconstruct based on virus-induced gene silencing (VIGS) technique was used to knockdown theT6ODM expression. In this regard, cDNA of T6ODM was prepared using specific primers andthe results of reaction were sequenced for confirmation. Then, a part of resulted cDNA(silencing fragment) was selected for cloning in pTRV vector. The silencing construct wasinfiltrated to plant leaves via Agrobacterium tumefaciens. The presence of cp (coat proteine)gene of TRV vector was assayed by PCR and the positive TRV plants were screened foranalysis of gene expression by semi quantitative and real-time PCR. Results of gene expressionanalysis showed that transgenic plants had a significant decrease in expression (with averageamount of 83.71%), comparing to control plants.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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1
no.
2018
1
11
https://ijrfpbgr.areeo.ac.ir/article_116635_73066405f2d87f4d94af81d3eecce403.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.116635
Isolation and bioinformatics study of TbJAMYC transcription factor involved in biosynthesis of Taxol from Iranian yew
D.
Jafari
Ph.D student of medical biotechnology, Medical Biotechnology Department, School of Allied Medicine, Iran
University of Medical Sciences, Tehran, Iran.
author
F.
Dehghan Nayeri
Corresponding author, Assist. Prof., Faculty of Agricultural and Natural Sciences, Biotechnology Department, Imam Khumaini
International University, Qazvin, I.R. Iran. Email: nayeri@alumni.ut.ac.ir,
author
text
article
2018
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An anti-cancer drug called Taxol, or paclitaxel is one of the secondary metabolites of yewtree (Taxus bacatta). Taxol is one of the most important and most useful anticancer drugs in thetreatment of lung cancer. Taxol prevents the depolymerization of microtubules and inhibits celldivision in tumor tissues. JAMYC is a transcription factor from MYC family with a DNAbindingmotif called bHLH. The gene involved in Taxol biosynthesis has been identified inother yew species. The aim of this study was isolation and cloning of TbJAMYC gene andbioinformatics analysis of its structure. Initially to obtain yew seedling, embryos were removedfrom seeds of the species and cultured on MS medium containing activated charcoal andascorbic acid. Total RNA was extracted from the seedlings and cDNA was synthesized fromextracted RNA. The gene of TbJAMYC was amplified by PCR from cDNA. Amplified fragmentcloned in pTG 19 vector using TA cloning method and sequenced. Bioinformatics analysis wascarried out by MODELLER and Pfam softwares. The cloning was confirmed by endonucleasedigestion and sequencing. TbJAMYC bioinformatics analysis was carried out by Pfam andMODELLER softwares that revealed three-dimensional structure of the DNA binding domain.These analyzes showed that TbJAMYC protein is a member of MYC transcription factorsfamily that binds to DNA and regulates gene expression by bHLH domain. TbJAMYCtranscription factor involved in the biosynthesis of valuable anticancer Taxol drug. Hence Taxolbiosynthesis pathway manipulation via genetic engineering can be a new way to increaseproduction of the metabolite in yew plant.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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1
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2018
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22
https://ijrfpbgr.areeo.ac.ir/article_116636_0565edd04cd8b9286b4027e4ebd4381a.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.116636
Evaluation of SO6 protein in Saponaria officinalis L. callus
H.
Honari
Corresponding author, Associ. Prof., Biology Science and Technology Center, Imam Hossein University, Tehran, I.R. Iran.
Email: Honari.hosein@gmail.com
author
S.M.
E'temad Aubi
PhD Student of nanobiotechnology, Biology Science and Technology Center, Imam Hossein University, Tehran, I.R. Iran
author
F.
Esmaili
M.Sc., Biotechnology, Islamic Azad University, Science and Research Branch, Tehran, I.R. Iran
author
M.
Abdollahi
M.Sc. Student of biology, Biology Science and Technology Center, Imam Hossein University, Tehran, I.R. Iran
author
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2018
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Soapwort (Saponaria officinalis L.) is a native plant to central and southern Europe. It is animportant and rare plant species in Iran. The species has numerous applications in medicinal,agricultural and health fields. Saponins is one of the bioactive compounds in the species that isused in chemotherapy. Tissue culture is one of the ways of proliferation of the species that isused for production of active ingredients. Saponin has ten isoforms that are different toinhibition of translating ribosomes and cytotoxicity. SO6 is one of the isoforms of saponins.Presence of SO6 protein in soapwort callus was studied through plant tissue culture anddifferent concentrations of growth regulators and environmental factors. After achieving successin tissue culture, protein was extracted from its callus and presence of SO6protein was confirmed by western blot analysis and measured by ELISA. Results showed that seedgermination was possible through using appropriate treatment and soapwort root callus had thehighest amount of SO6 protein.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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1
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2018
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https://ijrfpbgr.areeo.ac.ir/article_116637_96d113ff4501c6c3fa55cd18a296820b.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.116637
Genetic architecture of a Persian oak plant population based on progenies seedling characteristics
H.
Mirzaie-Nodoushan
Corresponding author, Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension
Organization (AREEO), Tehran, I.R. Iran. Email: nodoushan2003@yahoo.com
author
J.
Hosseinzadeh
Associ. Porf., Research Center of Agriculture and Natural Resources of Ilam, Agricultural Research, Education and Extension
Organization (AREEO),Tehran, I.R. Iran.
author
S.
Mehrpur
Assist. Porf., Islamic Azad University, Qom, I.R. Iran
author
P.
Panahi
Associ. Porf., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization
(AREEO),Tehran, I.R. Iran.
author
D.
Mehdifar
M.Sc., Research Center of Agriculture and Natural Resources of Lorestan, Agricultural Research, Education and Extension
Organization (AREEO),Tehran, I.R. Iran.
author
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2018
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Persian oak (Quercus brantii Lindl.) with a vast habitat in Zagros mountain range, has aspecific importance in Iran, based on economic, sociologic and environmental aspects. Acomplex of human and non-human factors caused to show genetic erosion in several plantpopulations of the species. Therefore, evaluation of genetic variation and architecture of plantpopulation could be informative for forest managers and breeders. The research was performedto evaluate genetic potentials of a plant population of the species located in Lorestan provinces,west of Iran. Seed was collected randomly on twelve single plants to be used in assessment ofthe parent plants by a progeny test experiment. The seeds were evaluated as twelve half-sib andFull-sib families planted in a completely randomized block design with three replications.Biometric parameters were converted to genetic parameters using nested mating design model,by which genetic variance components and heritability values were estimated. The studiedfamilies showed a range of 6 to 92 percent establishment. Analysis of variance revealedsignificant difference between the parents based on all of the recorded traits. Majority of thevariance components was observed between the studied families, for which heritability of thecharacters was also strong, varying between 57% on collar diameter, to 95% on leaf length.Therefore, the studied oak plant population has the capability to be used for selecting suitablematerials for seed orchard establishment as well as performing other breeding projects.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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1
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2018
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43
https://ijrfpbgr.areeo.ac.ir/article_116638_b556322ad198b173077e1e462ce31fd2.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.116638
Effect of culture media and growth regulators on micropropagation of medicinal plant Urtica dioica L.
M.
sedaghati
Corresponding author, Ph.D. Student, Department of Biology, Payame Noor University, Tehran, I.R. Iran E-mail: sedaghati@rifr-ac.ir
author
M.H.
Assareh
Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran
author
M.A.
Ebrahimi
Assoc. Prof. Department of Agricultural Biotechnology, Payame Noor University, Tehran, I.R. Iran
author
S.M.
Hesamzadeh-Hejazi
Assoc. Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran
author
H.
Sobhanian
Assis. Prof. Department of Biology, Payame Noor University, Tehran, I.R. Iran
author
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article
2018
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Urtica dioica L. is dioecious plant and cross pollination with active components and special medicinal properties with economic values. Due to high level of heterozygosity of the species, homogeneity in fiber production and harvesting of selected plants is not guaranteed by seed propagation. For this reason micropropagation and asexual proliferation is suggested for production of selected plants. For micropropagation of the species, apical buds of Nettle shoots were used as explant. After sterilization, the explants were transferred into MS medium containing BAP, 2iP and IBA. Then, to evaluate the culture conditions, the explants were located on culturing mediums (MS, DKW, WPM) with different concentrations of growth regulators (IBA, 2,4-D, BAP, KIN, 2iP , TDZ) in a factorial experimental model based on a completely randomized design with three replications. The best medium for shooting initiation was WPM culture. Between rooting treatments, the best root number and secondary roots belonged to KIN (0.5 mgl-1) + IBA (0.5 and 1 mgl-1) and BAP (0.5 mgl-1) + IBA (0.5 mgl-1) treatments, respectively. Then rooted plantlets transferred to the greenhouse and successfully were acclimatized (55.5%).
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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1
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2018
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52
https://ijrfpbgr.areeo.ac.ir/article_116655_d00194a63a523b798a28be76a4e869c4.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.116655
Effective factors on micropropagation of medicinal plant of Satureja khuzistanica
L.
Mirjani
Ph.D. student, College of Biological Sciences, Kharazmi University, Tehran, I.R. Iran.
author
A.
Salimi
Corresponding author, Assoc. Prof., College of Biological Sciences, Kharazmi University, Tehran, I.R. Iran,
author
M.
Matinizadeh
Assoc. Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran.
author
K.
Razavi
Accis. Prof., National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, I.R.Iran.
author
M.
Shahbazi
Accis. Prof., Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, I.R. Iran.
author
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2018
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Satureja khuzistanica Jamzad is an endemic species of Iran. The species is an endangered and valuable medicinal plant. In order to propagate through micropropagation, lateral buds were collected from Lorestan province, region of Pol of Dokhtar. The best treatment of sterilization of the explants was 0.1% HgCl2 for 6 minutes. The best medium for shoot regeneration was MS with BA, 2iP and IBA 0.3, 0.3 and 1 mg/l, respectively. Applying 12 different treatments, the mentioned medium was better than other treatments in term of the coefficient of elongation, leaf elongation and leaf greenness. The best medium for root regeneration was MS with 1 mg/l IBA and 0.1 mg/l NAA. It initiated the highest number of primary and secondary roots. Various combinations of different soils were evaluated for survival during the acclimatization, and sand: soil: peat: perlite at a ratio of 1: 1: 1: 1 showed the highest percentage of survival and growth. The best propagation method of the species was developed and the propagated plants could be transplanted into the habitat of the species.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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1
no.
2018
53
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https://ijrfpbgr.areeo.ac.ir/article_116656_63b4d5dc20b083b90df86399449f879e.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.116656
Asexual regeneration of (Zhumeria majdae L.) by Bud culture
M.
Emam
Corresponding author, Assist. Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran. Email: emam@rifr-ac.ir
author
L.
Mirjani
M.Sc., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran.
author
M.
Hesamzade Hejazi
Assoc. Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO),Tehran, I.R. Iran.
author
M.A.
Soltanipoor
Assist. Prof., Department of Forest, Agricultural and Natural Resources Research Center of Hormozgan, Agricultural Research, Education and Extension Organization (AREEO), Bandarabbas, I.R. Iran.
author
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2018
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Zhumeria majdae is one of the most important aromatic plant species from Lamiaceae family. The valuable species has medicinal and industrial applications. It is an endemic and endangered species of Iran. Therefore, tissue culture techniques are proposed for propagation and conservation purposes of the species. Usual regeneration of the species is by seed. Apical buds of seedlings from Hormozgan genotypes were sterilized by HgCl2 0.1% solution for 5 minutes during spring season. The best medium for shoot proliferation was WPM containing BA (0.5 mg/l), 2iP (0.3) and IBA (0.1 mg/l). The highest rooting of the shoots was obtained by WPM supplemented with 1 mg/l of IBA. The plants were acclimatized in greenhouse after transferring in soil. The protocol of this research could be used for culture and developmental programs of the species in its original habitat.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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1
no.
2018
63
70
https://ijrfpbgr.areeo.ac.ir/article_116657_416407eee430e53a6640cd0dfd7d512d.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.116657
Regeneration of an endangered Fritillaria (Fritillaria raddeana) species via petal and leaf explants
S.
Salahi Sadr
Ph.D. Student, Department of Horticultural Sciences, University Campus 2, University of Guilan, Rasht, I.R. Iran.
author
H.
Zakizadeh
Corresponding Author, Assist. Prof., Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, I.R. Iran. Email: Zakizadeh@guilan.ac.ir
author
M.R.
Naghavi
Prof., Dept. of Agronomy and Plant Breeding, Agricultural & Natural Resources College, University of Tehran, Karaj, I.R. Iran
author
J.A.
Olfati
Assoc. Prof., Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, I.R. Iran
author
text
article
2018
per
Fritillaria raddeana is one of the most fascinating wild flowers, belonging to Lilliaceae family, native to Iran. It is an important ornamental-medical bulbous plant, tolerant to arid conditions and stony slopes but facing extinction. There have been very few reports exist, so any studies on the species would be of great importance. Regardless of mutation occurrence, successful regeneration will be a prerequisite for further researches that must be done on the endangered plant species. This research presents an in vitro callus induction and indirect regeneration of F. raddeana via culturing petal and leaf. The leaf (before flowering) and petal (green to yellow flower buds) explants excited from plant and surface sterilized and were cultured on MS media containing different concentrations of Auxin and Cytokinin. The explants were first kept at darkness and 18ºC for callus induction, then were transferred to light condition with the same temperature. The temperature was raised to 20ºC, when the calli sub cultured into regeneration media. Although due to the low amount of bacterial and fungal contamination, conservation of parent plants, adequate and ready available explants, both petals and leaves are acceptable explants, among them petal showed better and quicker response to variable plant growth regulators. The best medium for callus formation (82.66%) and indirect regeneration (36.66%) via petal explants was MS medium supplemented with 2 mgl-1 TDZ and 0.5 mgl-1 IBA. Therefore, the mentioned plant growth regulators were more suitable than the others for indirect regeneration of F. raddeana.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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1
no.
2018
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https://ijrfpbgr.areeo.ac.ir/article_116658_d8eac7692e23c2f86231ad548ddd4b07.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.116658
Genetic variation and heritability of Amygdalus scoparia Spach. populations in Fars province
M.
Babaeiyan
PhD. student., University of Agriculture and Natural Resources, Gorgan, I.R. Iran
author
D.
Azadfar
Corresponding author, Assoc. Prof., College of Forest Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I.R. Iran, Email: azadfar.d@gmail.com
author
M.H.
Pahlevani
Assoc. Prof., College of Plant Production, Gorgan University of Agriculture Sciences and Natural Resources, Gorgan, I.R. Iran
author
Z.
Saedi
PhD graduates, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I.R.Iran
author
M.H.
Arzanesh
Assist. Prof., Agriculture and Natural Resources and Education Center of Golestan province, Gorgan, I.R. Iran
author
text
article
2018
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Amygdalus scoparia spach. a multi-purpose species which is growing in most of vegetation regions of Iran. Knowledge of genetic variability and heritability is essential prerequisite for conservation, forest development and sustainable use of forest plants. Genetic parameters on early growth were estimated in open-pollinated combined provenance-progeny tests. The experiment was conducted based on a nested model using randomized complete block design with 7 population, 10 mother trees per population 7 individuals per subplot and 3 replications in nursery of Kohmareh-Sorkhi of Fars (1660 m a.s.l.). Significant differences among the populations and mother trees within populations were observed for all of the studied traits, except for the taproot length among mother trees within populations. Evaluation of components of variance showed that the population effects (ranging from %5.9 to % 34.2) contributed to total variance more than mother trees within populations effects (ranging from%1.8 to %11.1). Additive genetic variation coefficient in populations varied from 0 to 58.6%. In general, Khonj and Kavar populations performed better growth. Heritability was low and not yet stable for some traits in some populations. Therefore, selection should be postponed until the heritability values reach to relatively high and stable values.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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1
no.
2018
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https://ijrfpbgr.areeo.ac.ir/article_116659_a2cf373a21fc341fe3c87ac1eb1fb4da.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.116659
Assessment of genetic diversity of Zataria multiflora ecotypes by RAPD and ISSR
H.
Bibak
Corresponding author, M.Sc., Department of Biology, Faculty of Science, University of Jiroft, Jiroft, I.R. Iran. Email: hbibak@ujiroft.ac.ir
author
K.
Aghaabbasi
PhD student, Department of Agriculture Biotechnology, Guilan University, Guilan, I.R. Iran.
author
text
article
2018
per
Assessment of genetic diversity and germplasm classification of plant species are important activities in plant improvement and management of genetic resources. Regarding observed variation on various traits of Zataria multiflora Bosis. random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to assess genetic diversity of the species in Iran. Leaves were collected on thyme plants of 15 different regions of Kerman,Sistan and Balochistan, Boushehr, Fars, and Khuzestan provinces. Leaf DNA extraction was carried out by cetyltrimethyl ammonium bromide (CTAB) method with small modifications. Ten RAPD markers and 10 ISSR markers with sharper bands in polymerase chain reaction (PCR) were used for data analysis. NTSYS-pc software and dice similarity coefficient, as well as Unweighted Pair Group Method with Arithmetic Averages (UPGMA) were used to draw the related dendrogram. Cophenetic correlation coefficient was calculated and a two-dimensional graph was created based on principle component analysis (PCA) results. Range of the bands produced by the primers varied from 120 to 3100bp. The 15 selected regions were classified into three separate groups. The established clusters were in a good harmony with geographical conditions. Entirely the markers produced 207 bands with polymorphism percentage of 86.9. The results of the dice similarity coefficient produced by NTYSIS suggested that genetic similarity of thyme varied between 0.4390–0.8460. The most genetic similarity was observed between Andimeshk and Eizeh samples and the least similarity was observed between Iranshahr and Jiroft samples. Results of principle components analysis and cluster analysis were similar. Cophenetic correlation coefficient was 74.60%. The results suggested that RAPD & ISSR markers are useful for investigating genetic variability of Zatraia multiflora germplasm.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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1
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2018
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https://ijrfpbgr.areeo.ac.ir/article_116660_34ec939085833d27b35c5bcb175de670.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.116660
Effect of juvenile tissue and plant growth regulators on in vitro shoot proliferation of Pronus avium L.
A.
Jafari Mofidabadi
Corresponding author, Assoc. Prof., Agriculture and Natural Resources Research Centre of Gorgan, Agricultural Research,
Education and Extension Organization (AREEO), Gorgan, I.R. Iran. Email: jafarimofidabadi@gmail.com
author
M.
Kamandloo
M.Sc., Agriculture and Natural Resources Research Centre of Gorgan, Agricultural Research, Education and Extension
Organization (AREEO), Gorgan, I.R. Iran.
author
A.R.
Nazari
-M.Sc., Agriculture and Natural Resources Research Centre of Gorgan, Agricultural Research, Education and Extension
Organization (AREEO), Gorgan, I.R. Iran.
author
text
article
2018
per
Propagation of Pronus avium L. through conventional micropropagation is essential for forestdevelopment and production of scion for grafting of commercial Pronus varieties. Formicropropagation and production of juvenile tissue as an initial source of proliferation, matureembryo explants were used. For in vitro shoots preparation, isolated embryos of mature treeswere transferred into MS medium containing 30 and 60 g/l sucrose. There were no significantdifferences between MS media containing 30 and 60g/l for percentage of embryo germinationusing t-Student test. Factorial analysis of collected data for further shoot proliferation indicatedthat there were highly significant differences between the used growth regulators for in vitroshoot length and number of produced explantsatα=0.01level. The highest average shoot length(5.86 cm) and the highest mean number of explants production were observed on MS mediumsupplemented with 4 mg/l BA and 0.2 mg/l IBA. Plantlet at 4 to 5 cm in height were rooted inMS hormone free medium and after successful acclimatization transferred to a green-house.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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1
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2018
114
120
https://ijrfpbgr.areeo.ac.ir/article_116665_3e802e677e20b31127377f8198fee21e.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.116665
Effects of different growth regulators, gelling agents and carbon sources on in vitro growth of Berberis vulgaris var. asperma
Y.A.
Saadat
Corresponding author, Associate Prof., Fars Research and Education Centre for Agriculture and Natural Resources,
Shiraz, I.R.Iran E-mail: y.saadat1336@gmail.com
author
A.
Abbasi
B.Sc. Fars Research and Education Centre for Agriculture and Natural Resources, Shiraz, I.R.Iran
author
text
article
2018
per
Berberis vulgaris var. asperma, is a medicinal plant of Iran and lack of healthy vigorousseedlings is the main limitation of barberry cultivation. This research was carried out to developa suitable technique for mass micropropagation of the species. Effects of different plant growthregulators, different gelling agents and different carbon sources on in vitro growth of barberrywere studied in several experiments. Based on the results of the experiments, nodal segmentscontaining 1-2 axillary buds of semi-hardwood shoots were the best explants for establishmentof clean in vitro cultures. Surface disinfection of plant materials originated from adult trees wasperformed with immersion in 2 gl-1 of Benomyl solution for one hour, then 1% solution ofcommercial bleach for 15 minutes and rinsed three times with sterile distilled water. Explantscultured on media solidified with Phytagel were significantly better than the media withDifcoBacto agar and Agargel for shoot production. Addition of 3 gl-1 activated charcoal resultedin control of nutrient medium and explants browning, improvement of survival and in vitrogrowth of the explants. DKW medium containing 2 mgl3 gl-1 activated charcoal and solidified with 2.5 gl-1-1 BA, 0.5 mgl-1 IBA , 2 mgl Phytagel was optimum for in vitro growth of barberry and recommended for shoot multiplication of the species. Rooting experiment wascarried out using a two phase procedure, root induction in media with high concentration ofIBA, followed by transferring to growth regulators free medium for root development. Norooting was observed and further research is needed.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
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1
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2018
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131
https://ijrfpbgr.areeo.ac.ir/article_116666_b8f941faee1dfcfc0760dc240fca4886.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.116666
Variation of yield and morphological traits in several Iranian populations of Agropyron pectiniforme
A.A.
Jafari
Corresponding author, Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran., E-mail: aajafari@rifr-ac.ir
author
M.
Jafari
M.Sc. Student, Islamic Azad University- Borujerd Branch, Borujerd, I. R. Iran
author
S.
Nakhjavan
Asist. Prof., Islamic Azad University- Borujerd Branch, Borujerd, I. R. Iran
author
S.
Afkar
Asist. Prof., Payame Noor University- Lorestan Branch, Khorramabad, I. R. Iran
author
text
article
2018
per
Agropyron pectiniforme having moderate to high yield and quality traits is cultivated forpasture regeneration and forage production in rangelands of Iran. In order to study geneticvariability in Agropyron pectiniforme based on morphological traits, 16 populations wereexamined in the field trial using a complete block design with three replications during 20042005in Karaj, Iran. Data were collected on heading date, plant height, tiller number, spikelength, forage yield, seed yield and thousand grain weights. Results showed significantdifferences between the populations for all of the recorded traits (P≤0.01). Populations Zangan487,Tabriz-707 and Gorgan-7565 with averaged values of 96 to 119 g/plant and 25 to 33g/plant had higher forage and seed production, respectively. Among them, Zangan-487 hadearly maturity date and Tabriz-707 had higher seed thousand grain weights. Phenotypiccorrelation between forage and seed yield was positively significant (P<0.01). Both traitspositively correlated to plant height, tiller number and spike length. The populations wereclassified into three groups based on cluster analysis. Cluster number 1 contained populationswith late maturity and moderate yield. The populations in cluster 2 had higher forage and seedproduction and populations in cluster 3 were early maturity and low productivity. Distancebetween the clusters were assessed using principal components analysis (PCA) andMahalanobis Distance. Result showed higher genetic distance between clusters 2 and 3. It wassuggested to use populations located in the separate clusters for improving composite varieties.The population Zangan-487 having higher both forage and seed yield was introduced aspromising population for future research. Result of mantel correlation test between population’sgeographical origin and matrix genetic distance of morphological traits was not significant. Thisfinding indicated a lack of association between genetic variation and their geographicdistribution. It was concluded that morphological traits provide important information for thegenetic conservation and utilization of future breeding programs in A. pectiniforme.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
v.
1
no.
2018
132
142
https://ijrfpbgr.areeo.ac.ir/article_116667_1545e66444acc59211b3c97a0363f65d.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.116667
Identification of effective traits for improving forage yield in three species of Agropyron genus
B.
Rahnemoun
M. Sc. Student, Department of Plant Genetics and Production, Faculty of Agriculture, University of Maragheh, Maragheh, I.R.
Iran
author
H.
Hatami Maleki
Assist. Prof., Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, I.R. Iran.
author
R.
Mohammadi
Assist. Prof., Branch for Northwest & West region, Agricultural Biotechnology Research Institute of Iran
author
M.
Nouraein
M. Sc. Student, Department of Plant Genetics and Production, Faculty of Agriculture, University of Maragheh, Maragheh, I.R.Iran
author
text
article
2018
per
In order to determine relationship among forage dry matter yield and soeveral associatedagronomic traits in Agropyron genus, 31 populations of three Agropyron species (A. desertorum, A. cristatum and A. elongatum) were evaluated in a randomized complete block design (RCBD) with three replications in research farm of Agricultural Biotechnology Research Institute of Northwest and West region of Iran- Tabriz. Correlation coefficients analysis showed that dry forage yield in A. elongatum, had significant positive correlation with canopy diameter and negative correlation with number of days to pollination. For A. desertorum and A. cristatum dry forage yield had also significant and positive correlation with number of stems and negative correlation with flag leaf width. Using stepwise regression analysis, in A. elongatum, canopy diameter and number of days to pollination and in A. desertorum and A. cristatum, number of stem and panicle length were entered into regression model. Path analysis for dry forage yield as a dependent variable, revealed that in A. elongatum canopy diameter had positive direct effect (0.61) and number of days to pollination had negative direct effect (-0.46) and in A. desertorum and A. cristatum, number of stems had positive direct effect (0.73) and panicle length had negative direct effect (-0.44) on dependent variable. Results showed that canopy diameter and the number of days to pollination in A. elongatum, and the number of stem in the A. desertorum and A. cristatum, had great effect in determining forage dry matter yield and therefore, could be recommended as indices in screening suitable genotypes.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
26
v.
1
no.
2018
143
154
https://ijrfpbgr.areeo.ac.ir/article_116668_d56e30f32c208b84e6c247fe856e4675.pdf
dx.doi.org/10.22092/ijrfpbgr.2018.116668