Production of pharmaceutical active ingredients via hairy root induction
of Birch (Betula pendula)
Razieh
Jafari Hajati
Ph.D student, Faculty of forest sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I.R.Iran
author
Vahideh
Payamnoor
Assis. Prof., Faculty of Forest Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I.R.Iran
author
Kamal
Ghasemi Bezdi
Assis. Prof., Faculty of Forest Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I.R.Iran
author
Najmeh
Ahmadian Chashmi
Assis. Prof., Department of Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, I.R.Iran
author
text
article
2016
per
Betula pendula Roth produces a range of biologically active terpenoids such as betulin and betulinic acid. The compounds with cytotoxic effects which have been used for their medicinal properties. Agrobacterium-infected hairy roots are characterized by high growth rate and genetic stability that can beprovided noticeable amounts of the biological material. In this study, production of hairy roots by B. pendula was investigated on different explants, media and A. rhizogenes strains. Growth rate of hairy roots were studied during 20 days and the terpenoids content (betulin & betulinic acid) were assayed by HPLC. Hairy root cultures were established 20 days after infection by infecting bark segments of B. pendula by Agrobactrium strains of C58C1 and LB9402 in WPM medium. Growth of hairy root by lines of C58C1 strain (D and E lines) and one line from LB9402 strain (LB1) were significantly better than others. The most betulin content was observed in the hairy roots, 8 days after culture, which was about 0.47 mg g-1 dry waight (DW). Furthermore, the most amount of antioxidant activity (75%) was assessed on E line roots, 20 days after cultures. Although the highest content of betulinic acid (0.6 mg g-1 DW ) was obtained by LB1 lines but betulin content and antioxidant activity were considerably less than other hairy root lines. Results indicated that due to higher relative performance, E line of hairy roots could be used for analysis of terpenoids and pharmaceutically important metabolites in future studies.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
24
v.
2
no.
2016
165
176
https://ijrfpbgr.areeo.ac.ir/article_109403_f43641edd32adf6d8d71c14262039146.pdf
dx.doi.org/10.22092/ijrfpbgr.2016.109403
Genetic diversity analysis of Ziziphora tenuior L. using SCoT markers
Navid
Zamani
College of Environment and Natural Resources, Khatam Alanbia University of Technology, Behbahan
author
Vahid
Zamani
Laboratory of Alpine Ecology, Joseph Fourier University, Grenoble, France
author
Khaled
Mirzaei
M.Sc., University of Kurdistan, Sanandaj, I.R. Iran
author
text
article
2016
per
Twenty five SCoT primers were employed to investigate genetic diversity of 42 samples of Ziziphora tenuior from different geographical regions of Iran. A total of 209 fragments were amplified and 66% of them were polymorphic. Most amplified polymorphic fragments (10) were related to a primer named SCoT-54, while another primer named SCoT-20 showed the lowest (2) amplified polymorphic bands. Average number of amplified bands for primers was 8.36, while average number of polymorphic bands was 5.53. Polymorphic information content and discrimination power (PICD) varied between 0.217 in SCoT-31 and 0.074 in SCoT-30 with an average of 0.156. Similarity matrix was generated using Jaccard coefficient, Cluster analysis was carried out by UPGMA algorithm and principle coordinate analysis. According to the cluster analysis within the similarity coefficient of 0.7, Ziziphora samples were divided into 7 groups. Based upon similarity matrix, the lowest similarity (0.59) was found for two samples from Naein and Yasuj. The highest similarity (0.98) found between two samples from Damghan and Garmsar within the same geographical region. Analysis of molecular variance (AMOVA) suggested significant genetic differences within the samples. In fact, 89% of variation occurred within the species, whereas 11% of the variation was observed among the samples of Ziziphora tenuior. The results finally indicated moderate genetic diversity among the studied genotypes.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
24
v.
2
no.
2016
177
189
https://ijrfpbgr.areeo.ac.ir/article_109406_a24f16e2130fc2a3d4e2c64ed319a080.pdf
dx.doi.org/10.22092/ijrfpbgr.2016.109406
Study of zinc effect on limonene synthase gene expression in cumin by Real Time PCR
Mohammad
Toghroli
M.Sc., Department of Biotechnology, Faculty of Agriculture, Islamic Azad University, Sabzevar, I.R. Iran
author
Jafar
Vatandoost
Corresponding author Assis. Prof., Department of Biology, Faculty of Science, Hakim Sabzevari University, Sabzevar, I.R. Iran,
E-mail: j.vatan@hsu.ac.ir
author
M.H.
Asadi
Assis. Prof., Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, I.R. Iran.
author
text
article
2016
per
Zinc as an essential element for plant growth, plays an important role in many metabolic processes of plants, such as limonene synthase gene expression pathway. Limonene synthase is considered as an important enzyme in volatile oil biosynthesis that plays significant role in plant defensive reactions. In the present study, limonene synthase gene expression in Cumin was evaluated in presence of different concentration of Zinc elicitors based on a completely randomized design. Total RNA was extracted and cDNA synthesis was performed and Real Time PCR quantity assay method was used for limonene synthase gene expression. Results of the comparison of gene expression with Duncan's multiple range tests in different concentrations showed that in treated plants, there was a direct correlation between increasing of Zinc concentration up to 100 µM and limonene synthase gene expression. Limonene synthase gene expression by increasing production of monoterpens may produce more volatile oils in plant species and thus protects the plant against herbivores.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
24
v.
2
no.
2016
190
198
https://ijrfpbgr.areeo.ac.ir/article_109409_8aac0bbf624d68b91edb44412b1230c3.pdf
dx.doi.org/10.22092/ijrfpbgr.2016.109409
Genetic variation of Moringa peregrina populations based on seed storage proteins
Hossein
Mirzaie-Nodoushan
Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran.
author
Zeinab
Nazari
M.Sc., Payam Noor University, Tehran, I.R. Iran
author
Fereshteh
Asadicorom
M.Sc. Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran
author
Gholam Reza
Bakhshi-Khaniki
Prof., Payam Noor University, Tehran, I.R. Iran
author
text
article
2016
per
Moringa peregrine (Forssk.) Fiori is a valuable species with a vast utilization in nutritional, medicinal, health, and industrial aspects which is under genetic erosion and extinction threat both in Iran and all around its global habitats. Due to its importance and roles, study on its biological aspects such as genetic variation may accelerate its development. For this reason, 11 single trees of four different plant populations of the species, collected from South part of Baluchestan province, of Iran, were used in this study. Extracting, seed storage proteins of the genotypes, SDS-PAGE method of electrophoresis was used to evaluate genetic variation between and within the populations on macromolecule protein level. Performing electrophoresis, fixation and staining the protein bands, presence, or absence of the protein bands were investigated. Regarding the protein bands, based on qualitative criterion, there were not remarkable differences between the studied genotypes and regions to be used for discriminating between the studied genotypes and populations. In contrast with the qualitative differences there were plenty of quantitative differences between the studied genotypes and populations. Since gene flow and genetic interchange between the natural populations through seed transfer is rarely happening in the nature, it is expected to have plant populations with less interchange and more genetic differentiation.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
24
v.
2
no.
2016
199
207
https://ijrfpbgr.areeo.ac.ir/article_109410_0cbcafccfac75076dbe7d565f1008a1e.pdf
dx.doi.org/10.22092/ijrfpbgr.2016.109410
A new subspecies of sea buckthorns (Elaeagnus rhamnoides (L.) A. Nelson)
from Iran based on molecular data
H.
Ahani
Natural Resources Office of Khorasan Razavi, Mashhad, I.R. Iran
author
H.
Jalilvand
Professor, Department of Forestry, natural resources faculty, Sari agricultural sciences and natural resources University, Iran
author
J.
Vaezi
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, I.R. Iran
author
S.E.
Sadati
- Assoc. Prof., Agriculture and Natural Resources Research Center of Mazandaran, Agricultural Research, Education and Extension
Organization (AREEO), Sari, I.R. Iran
author
text
article
2016
per
Sea buckthorn (Elaeagnus rhamnoides (L.) A. Nelson) is a medicinal, drought tolerant, nitrogen fixing and forest pioneer plant species. In this research, for the first time in Iran, molecular identification of the species distributed in different habitats of the country was carried out comparing to the known subspecies distributed worldwide. Specimens were collected from five habitats located in Alborz, Mazandaran, East and West Azerbaijan, and Qazvin provinces. Nuclear ribosomal ITS marker was used in the molecular study. After DNA extraction and PCR application, sequences obtained in this study were aligned with those obtained from Gene-Bank belonging to all sea buckthorn subspecies. The aligned sequences were analyzed using Bayesian and TCS phylogenetic methods. Results showed that the specimens collected from Alborz, Mazandaran, East Azerbaijan and Qazvin belong to the Caucasian subspecies, E. rhamnoides subsp. caucasica, but the specimen sampled from West Azerbaijan with two mutations is a new subspecies. Therefore, it is introduced as E. rhamnoides subsp. azerbaijanica.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
24
v.
2
no.
2016
208
220
https://ijrfpbgr.areeo.ac.ir/article_109411_c834bff43d806bbbd7599d1027ec07b4.pdf
dx.doi.org/10.22092/ijrfpbgr.2016.109411
In vitro selection of Plantago psyllium L. for salt tolerance and changes of sodium, calcium and potassium levels at callus stage
A.
Safarnejad
Associ, Prof., Faculty member of Razavi Khorasan Agricultural and Natural Resources Research Center, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, I.R. Iran
author
M.
Shoorvarzi
M.Sc., Department of Tissue Culture Research, Branch of East and North-East Region of Iran, Agricultural Biotechnology Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, I.R. Iran
author
M.
Dalir
M.Sc., Department of Tissue Culture Research, Branch of East and North-East Region of Iran, Agricultural Biotechnology Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, I.R. Iran
author
text
article
2016
per
Plantago psyllium L. is from "Plantaginaceae" family and one of the most important medicinal plants with wide distribution in Iranian flora. In order to evaluate responses of Plantago psyllium L. to salinity, an experiment was conducted using tissue culture technique as well as somaclonal variation for identification and production of tolerant genotypes. The experiment was performed based on a completely randomized design with 12 treatments and 15 replications on each treatment. Seed germination percentage was 84%. Hypocotyl and root explants showed the highest and the lowest callus induction in non-saline (control) conditions respectively. In the terms of stress condition percentage of callus induction was decreased by increasing NaCl concentration on all of the three explants. Hypocotyl and root callus showed the highest and the lowest percentage of regeneration. Hypocotyl and root explants showed the highest and the lowest dry and fresh weight at all concentrations of NaCl. Also increment of NaCl concentrations led to increment of sodium and decrement of potassium ingredients of the callus. Amount of calcium varied by increasing NaCl concentration. Regenerated plants transferred to MS medium for root initiation. Then the seedlings were transferred to soil to set seed. Generally, in vitro selection is a suitable method for identification and selection of the tolerant genotypes to salinity in Plantago psyllium.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
24
v.
2
no.
2016
221
231
https://ijrfpbgr.areeo.ac.ir/article_109412_7152c0e1269352a9dda0a31af1831c62.pdf
dx.doi.org/10.22092/ijrfpbgr.2016.109412
Effects of growth regulators on in vitro callogenesis and regeneration
of black cumin (Nigella sativa)
S.
Hoseinpanahi
M.Sc., Agricultural Biotechnology, University of Kurdistan, Sanandaj, I.R.Iran
author
M.
Majdi
Assist. Prof., Department of Plant Breeding, University of Kurdistan, Sanandaj, I.R.Iran
author
G.
Mirzaghaderi
Assist. Prof., Department of Plant Breeding, University of Kurdistan, Sanandaj, I.R.Iran
author
text
article
2016
per
Black cumin (Nigella sativa) is one of the most important medicinal plants belongs to Ranunculaceae. In spite of its significance in pharmaceutical industry, tissue culture and micropropagation of the species is not well studied yet. Hence, based on the issue we aimed to study its callogenesis and regeneration on leave explants of the species. In vitro provided explants of leave were cultured on MS medium supplemented with NAA (0.5, 1, 1.5 mgL-1) and BAP (0.2, 0.5, 1 mgL-1). Half MS medium supplemented with 0.5, 1 and 1.5 mgL-1NAA and IBA were used for rooting the regenerated shoots. Results showed that higher percentage of callogenesis was observed on MS medium supplemented with 0.5 mgL-1NAA and 0.5 mgL-1 BAP. Shooting was observed on MS medium (1mgL-1BAP) and rooting on half MS (1 mgL-1NAA), respectively. Results indicated that callogenesis, shooting and rooting of black cumin are highly affected by hormonal combination levels.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
24
v.
2
no.
2016
232
242
https://ijrfpbgr.areeo.ac.ir/article_109413_bea90879a84cab382ba7d260295b8a08.pdf
dx.doi.org/10.22092/ijrfpbgr.2016.109413
Yield evaluation of new interspecific poplar hybrids introduced to Chaharmahal and Bakhtiari province for poplar plantation
A.
Jafari Mofidabadi
Assoc. Prof., Research Center of Agriculture and Natural Resources, Agricultural Research, Education and Extension Organization (AREEO), Gorgan, I.R. Iran
author
A.
Ghameri-Zare
Assoc. Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran
author
Y.
Iranmanesh
Assist. Prof., Research Division of Natural Resources, Chaharmahal and Bakhtiari Agriculture and Natural Resources Research and Education Centre, Agricultural Research, Education and Extension Organization (AREEO), Shahre Kord, I.R. Iran
author
text
article
2016
per
In order to introduce new poplar hybrid clones (“Mofid” and P. × albaeuphratica) for commercial wood production in different parts of Iran, ecological range of productivity of the hybrids have to be determined. Therefore, adaptability of the hybrid clones, "Mofid" and its reciprocal cross hybrid (P. × albaeuphratica), along with Populus alba L. and P. nigra, as local clones, were investigated. The experiment was conducted based on a randomized complete block design with three replications. Three hundred individual cuttings of the clones were planted in 100 square m experimental plots by spacing of 2 × 2 m. Analyses of variance was performed on quantitative and qualitative characters recorded during four years. Results indicated that there were highly significant differences between the clones at 0.01 level of probability. P. × albaeuphratica hybrid showed higher diameter at breast height (4.98 cm), trunk height (2.08 m) and total height (5.24 m) than others two clones. Low level of breast height diameter and total height were observed for P. nigra L. clone as a local poplar clone. Mofidwas sensitive to winter and spring cold conditions (below -30°C), While P. × albaeuphratica showed a high level of cold tolerance to the mentioned temperature.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
24
v.
2
no.
2016
243
248
https://ijrfpbgr.areeo.ac.ir/article_109414_e1fb2a8fd93bc6ac9bfbe5c6ee4274a7.pdf
dx.doi.org/10.22092/ijrfpbgr.2017.109414
Effects of drought stress on seed yield and yield components in drought tolerant synthetic cultivars and ecotypes of fennel (Foeniculum vulgare Mill.)
K.
Bahmani
PhD Student, University of Tehran, College of Aburaihan, Tehran, I.R. Iran.
author
A.
Izadi Darbandi
Assoc., Prof., Department of Agronomy and Plant Breeding Sciences, University of Tehran, College of Aburaihan, Tehran, I.R. Iran
author
A.
Akbari
M.Sc., Department of Agronomy and Plant Breeding Sciences, University of Tehran, College of Aburaihan, Tehran, I.R. Iran
author
text
article
2016
per
Effects of drought stress on seed yield and yield components in fennel genotypes was evaluated at research field of college of Aburaihan, University of Tehran, Iran during 2013. Experiment was carried out in split plot arrangement based on a randomized complete block design (RCBD) with three replications. Normal irrigation and drought stress treatments were assigned to main plots and fennel genotypes including two drought tolerant synthetic cultivars (early and medium maturity synthetic cultivars) and their four elite parents (Fasa, Meshkinshahr, Fozveh and Hashtgerd) were randomized in subplots. Analysis of variance showed that effects of drought stress on number of umbels, number of umbellate, seed yield and biological yield were significant. Drought stress reduced all measured traits and seed yield (40%) was affected the most. Result showed significant differences among the genotypes for all of the studied traits. Mean comparison of the genotypes showed that the highest seed yield belonged to Fozveh (1125 kg/ha) in normal condition and lowest seed yield belonged to Fasa (434 kg/ha) under drought stress. Early and medium maturity synthetic cultivars had the highest seed yield in drought stress conditions. Stress tolerance score (STS) for genotypes were calculated. Early and medium maturity synthetic cultivars with the highest amounts of STS were introduced as the best genotypes with drought tolerant. Finally based on the results, the cultivars in environments with variable climate, especially water shortage, can produce satisfactory yield.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
24
v.
2
no.
2016
249
263
https://ijrfpbgr.areeo.ac.ir/article_109415_ea6b9bdcab48a5efaf035a4b7fa147eb.pdf
dx.doi.org/10.22092/ijrfpbgr.2016.109415
Effects of different concentrations of growth regulators and explants type on callus induction, embryogenesis and shoot regeneration of Origanum vulgare ssp. Gracile
B.
Hosseini
Associate Prof., Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, I.R. Iran
author
A.
Bighamat
M.Sc., Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, I.R. Iran
author
text
article
2016
per
Origanum vulgar ssp. gracile is one of medicinal plants belonging to Lamiaceae family. Carvacrol was detected as a dominant component of essential oils of the species. Effects of different concentrations of 2,4-Dichlorophenoxy Acetic Acid (2,4-D) in combination with benzyl adenine (BAP) were investigated on callus induction of various explants (Cotyledon, Hypocotyl, Node, Shoot Tip and leaf) of the species.Interaction between culture media and explant types was significant at 1% Level of probability. The highest percentage of callus induction (100%) was obtained on MS medium supplemented with 1 and 3 mg l−1 2,4-D on node explants. Maximum callus induction (93.3%) was observed on MS medium supplemented with 3 mg l−1 2,4-D. No callus induction (0%) was observed on control and MS medium supplemented with 0.1 mg l−1 BA on leaf explants. Higher percentage of embryogenic callus production was obtained on MS medium supplemented with 1 mg l−1 2,4-D in combination with 0.1 mg l−1 BAP on shoot tip explants. Percentage of embryogenic induction was the lowest on hormone free MS medium and media containing 0.1 mg l−1 BAP in all explants and MS media supplemented with 5 mg l−1 2,4-D on hypocotyl explant. By increasing 2,4-D concentration, embryogenesis was decreased and maximum embryogenesis percentage was obtained by shoot tip and node explants. Effects of BAP on shoot regeneration from explants, the highest shoot regeneration (100%) on nodes and shoot tip explants on hormone free media and MS media fortified with 1 mg l−1 BAP were obtained.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
24
v.
2
no.
2016
264
276
https://ijrfpbgr.areeo.ac.ir/article_109416_cbf67bcc33830fb9ecf9bfc4b2c9fce1.pdf
dx.doi.org/10.22092/ijrfpbgr.2016.109416
Stability analysis of essential oil yield in different populations of thyme
(Thymus kotschyanus) in different regions of Iran using GGE biplot
A.
Pourmeidani
PhD student in Plant Breeding, Faculty of Agriculture, Islamic Azad University, Science and Research, Tehran, I.R. Iran
author
H.
Maddah Arefi
Asso. Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran
author
A.A.
Jafari
Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, I.R. Iran
author
S.
Torabi
Assis. Prof., Faculty of Agriculture, Islamic Azad University, Science and Research, Tehran, I.R. Iran
author
M.
Mirza
Prof., Research Institute of Forests and Rangelands, Tehran, I.R. Iran
author
text
article
2016
per
To determine essential oil yield stability in different populations of thyme (Thymus kotschyanus), 20 plant populations were examined in normal conditions at 11 stations, consisted Damavand, Esfahan, Golestan, Hamedan, Khorasan, Markazi, Qom, Tabriz, Tehran, Yazd and Zanjan. Data were analyzed in split plot in location design. Based on combined analysis of variance, genotype × location interaction was significant at (p>1%). This showed differences of the studied populations in yield and response to different conditions in eleven locations. In Graphical analysis, 11 environments were divided into 3 Mega environments. In mega environments Khorasan, Qom, Markazi, Damavand, Yazd, Zanjan and Tehran, genotypes 5, 27, 51 and 54 showed special compatibility. Genotypes 3 and 17 in Tabriz and Hamadan mega environment and genotypes 10, 8, 70 and 50 in mega environment of Golestan showed special compatibility. Assessment based on essential oil yield and stability of genotypes showed that accessions 5, 54 and 29 were suitable accessions. Graphical analysis of relationships between the environments, Khorasan, Qom, Markazi and Damavand, formed a group environment. Khorasan, Tabriz, Isfahan and Golestan locations showed significant role in differentiation of the studied genotypes. Based on graphical analysis of relationship between the genotypes, genotypes of 54 (Naghade), 27 (unknown 1), 51 (Tehran), 56 (Zarand) and 5 (Qazvin 2) formed a high production group presenting special compatibility to mega environment of Khorasan, Qom, Markazi, Damavand, Yazd, Zanjan and Tehran. The mentioned genotypes could be used for thyme cultivation in the mentioned areas.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
24
v.
2
no.
2016
277
286
https://ijrfpbgr.areeo.ac.ir/article_109417_0bc3e2eacc3dc17e27e2c3f05d175545.pdf
dx.doi.org/10.22092/ijrfpbgr.2016.109417
Assessing heritability of agro-morphological characters and relationship between genetic diversity with geographical factors in Einkorn wild wheat populations collected from West and Northwest of Iran
A.R.
Pour-Aboughadareh
Ph. D, College of Agricultural & Natural Resources, Imam Khomeini International University, Ghazvin, I.R. Iran
author
M.
Moghaddam
Prof., College of Agriculture, University of Tabriz, Tabriz, I.R. Iran
author
S.S.
Alavikia
Assist. Prof., College of Agriculture, University of Tabriz, Tabriz, I.R. Iran
author
A.A.
Mehrabi
Assoc. Prof., College of Agriculture, University of Ilam, Ilam, I.R. Iran
author
text
article
2016
per
In order to evaluate heritability of agro-morphological characters as well as study of relationship between genetic diversity with geographical factors in Einkorn wild wheat populations (41 accessions consisted of 33 accessions of T. boeoticum and 8 accessions of T. urartu species), an experiment was conducted based on a randomized complete block design with three replications in research station of University of Tabriz, Iran during 2011-2012 growing seasons. Analysis of variance showed significant differences among the accessions for all of studied characters except numbers of total tiller and fertile tiller. Mean comparison also revealed significant differences between the two species in terms of all characters except leaf width as well as weight of total spikes and harvest index. For both of the species, heritability and variation coefficients indicated high level of genetic variation for agro-morphological characters. According to correlation coefficients, a positive and significant relation was found among the studied characters. However, only correlation between latitude and spike length as well as altitude and number of total tillers were positive and significant. Clustering of the accessions on the basis of all characters and geographical factors produced two main groups, so that in both dendrograms grouping of accessions were greatly consistent with each other. Accessions with closer altitude and latitude were allocated to similar groups. On the whole, remarkable level of genetic diversity among Einkorn wheat, especially accessions collected from Kermanshah and Lorestan provinces was revealed, implying special attention to germplasm of the areas is required.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
24
v.
2
no.
2016
287
304
https://ijrfpbgr.areeo.ac.ir/article_109418_48bc8ab4aa11f6f6dc285761d14a4431.pdf
dx.doi.org/10.22092/ijrfpbgr.2016.109418
Cytogenetic study of Plantago major populations in North West of Iran
Farzaneh sadat
ghasemi
M.Sc., Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, I.R. Iran,
author
A.
Jalili
Prof., Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO),
Tehran, I.R. Iran
author
S.S.
Mirzadeh Vaghefi
Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO),
Tehran, I.R. Iran
author
S.S.
Kazemi
M.Sc., Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO),
Tehran, I.R. Iran
author
text
article
2016
per
Plantago genus of Plantaginaceae family has 22 annual and perennial herbaceous species in Iran. Plantago major is one of common plants in wetlands. Chromosomal characteristics of seven populations of Plantago majorL. were studied. Seeds were collected from habitats of the species in NW of Iran. The seeds were grown in petri dishes. Root tip meristems were stained with Hematoxilin. Chromosome number of five populations was 2n= 12 and in two other populations was 2n=24. Base Chromosome number of the species was x=6. In studied populations, Karyotype formula had metacentric and submetacentric chromosomes. Chromosome size of the species varied between 1.25 to 2.87 µm. Ideogram of each population was made and karyotypic characteristics including chromosome number, long and short arm length, and arm ratio indices were also estimated. The species had symmetrical karyotype in all of the examined populations; According to Stebbins classification, six of the studied populations located in 1A class and one of the populations located in 2A class.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
24
v.
2
no.
2016
305
314
https://ijrfpbgr.areeo.ac.ir/article_109419_0614c45ce38885d6cf5145acdbed93b4.pdf
dx.doi.org/10.22092/ijrfpbgr.2016.109419
Evaluation of drought tolerance in alfalfa (Medicago sativa) accessions under normal and dryland farming system
farid
noormand moayyed
M.Sc. Agriculture and Natural Resources Research Center, Agricultural Research, Education and Extension Organization (AREEO), Tabriz, I.R. Iran
author
A.A.
Jafari
Prof., Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO),
Tehran, I.R. Iran.
author
A.
Razban Haghighi
M.Sc. Agriculture and Natural Resources Research Center, Agricultural Research, Education and Extension Organization
(AREEO), Tabriz, I.R. Iran
author
text
article
2016
per
In order to evaluate drought tolerance of alfalfa (Medicago sativa), 49 accessions of the species were examined in two separate experiments using simple lattice designs under normal and dryland farming system during 2005-2007 in Tabriz, Iran. Forage dry matter yield was evaluated during two years. Results of combined analysis showed significant differences among the genotypes and among the years. Means of forage dry matter yield were 9.01 and 4.94 ton/h under irrigation and dryland farming systems, respectively. Cluster analysis correctly distributed the genotypes in the relevant groups based on yield and STI Index. Group 3 with genotypes of 14, 25, 37, 32, 11, 13 and 10 with average yield of 6.42 ton/h and STI= 0.95 was recognized as drought tolerant group. Based on stress intensity SI=0.452, Stress Tolerance Index (STI) and Stress Tolerance (TOL), genotypes 14 (Khoramabad) and 32 (Faridan) with average values of 8.74 and 7.26 ton/h respectively had the highest production in dryland farming system, that were recognized as drought tolerant accessions.
Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research
Regional Information Center for Science and Technology (RICeST)
Islamic World Science Citation Center (ISC)
1735-0891
24
v.
2
no.
2016
314
322
https://ijrfpbgr.areeo.ac.ir/article_109420_3ae121ce9c3536708de46bae09afb293.pdf
dx.doi.org/10.22092/ijrfpbgr.2016.109420